Roman Yanko*

Department of Clinical Physiology of Connective Tissue, Bogomoletz Institute of Physiology National Academy of Sciences of Ukraine, Ukraine

*Corresponding Author: SRoman Yanko, Department of Clinical Physiology of Connective Tissue, Bogomoletz Institute of Physiology National Academy of Sciences of Ukraine, Ukraine

Received: August 31, 2023; Published: November 03, 2023

Abstract

Introduction: With age, the pancreas, like most other organs, undergoes a decrease in functional activity. Therefore, the search for methods that could activate the pancreas, if necessary, is an actual direction of research. One of these methods can be the use of the amino acid methionine, or preparations based on it.

Aim: To investigate the histo-morphological changes of the rat’s pancreas of various ages after the administration of L-methionine to them and to evaluate the prospects of its use as a means to correct pronounced age-related decreases in its function.

Materials and Methods: Experiments were performed on 48 male rats aged 3 and 15 months. Experimental animals, in addition to the standard diet, received L-methionine at a dose of 250 mg/kg of body weight every day for 21 days. Histological preparations were made from pancreas tissue according to the standard method. Morphometry was carried out using the computer program "Image J". Research was conducted in accordance with the provisions of the "European Convention for the Protection of Vertebrate Animals Used for Experimental and Other Scientific Purposes" (Strasbourg, 1986).

Results: At the end of the experiment, 15-month-old rats receiving L-methionine showed morphometric signs of increased exocrine functional activity (the area of acinus and the height of their epithelium increased, the nuclear-cytoplasmic ratio of exocrinocytes, and the number of nucleolus in cell nucleus increased, the amount of connective tissue decreased), as well as the endocrine part of the pancreas (the size of the Langerhans islets and the number of endocrinocytes placed in them increased). In 3-month-old animals, on the contrary, methionine reduced the activity of the endocrine part of the gland and almost did not change the histo-morphometric indicators of the exocrine part.

Conclusion: The obtained data can be used for the correction of pronounced age-related decreases in the function of the pancreas in adults and the elderly, as well as in the prevention of age-related chronic diseases of the gland. At the same time, receiving additional doses of methionine at a young age is not desirable.

Keywords: Pancreas; Histo-morphometry; Methionine; Age

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Citation

Citation: Roman Yanko. “Age-related Differences in the Effect of L-Methionine on Histo-Morphometric Indicators of Pancreas Activity in Rats".Acta Scientific Gastrointestinal Disorders 6.11 (2023): 02-08.

Copyright

Copyright: © 2023 Roman Yanko. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.