Abstract

  IgY is a predominant antibody found in reptiles, birds and amphibians. IgY is equivalent to IgG of mammals. In the present study, IgY antibodies against Shigella flexneri was produced by immunizing chicken with 0.2 ml of 3.6x10⁹ CFU ml^{-1 of formalin inactivated Shigella flexneri ATCC 9199 strain. Eggs were collected and IgY antibodies were harvested using PEG precipitation method. Yield of ~25 mg of IgY per egg yolk was recovered. Dot ELISA with immunized IgY showed reactivity with Shigella flexneri and also other Shigella species, while naïve IgY did not exhibit any reactivity. IgY cross-reactivity with other Shigella members indicate the prosect of using IgY in detection and neutralization of pathogenic Shigella strains.}

Keywords: Immunoglobulin Y; Egg Yolk; Shigella flexneri; Dot ELISA; UV Spectrum

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Citation

Citation: Prakash Narayana Reddy., et al. “IgY: A Promising Alternative to IgG in Immunodiagnostics".Acta Scientific Biotechnology 1.1 (2020): 18-21.

Copyright

Copyright: © 2020 Prakash Narayana Reddy., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.