Acta Scientific Biotechnology (ASBT)

Research Article Volume 1 Issue 1

IgY: A Promising Alternative to IgG in Immunodiagnostics

Kota Rohini Krishna, Krupanidhi Sreerama and Prakash Narayana Reddy*

Department of Biotechnology, Vignan’s Foundation for Science, Technology and Research (Deemed to be University), Vadlamudi, Andhra Pradesh, India

*Corresponding Author: Prakash Narayana Reddy, Department of Biotechnology, Vignan’s Foundation for Science, Technology and Research (Deemed to be University), Vadlamudi, Andhra Pradesh, India.

Received: December 11, 2019; Published: December 31, 2019



  IgY is a predominant antibody found in reptiles, birds and amphibians. IgY is equivalent to IgG of mammals. In the present study, IgY antibodies against Shigella flexneri was produced by immunizing chicken with 0.2 ml of 3.6x109 CFU ml-1 of formalin inactivated Shigella flexneri ATCC 9199 strain. Eggs were collected and IgY antibodies were harvested using PEG precipitation method. Yield of ~25 mg of IgY per egg yolk was recovered. Dot ELISA with immunized IgY showed reactivity with Shigella flexneri and also other Shigella species, while naïve IgY did not exhibit any reactivity. IgY cross-reactivity with other Shigella members indicate the prosect of using IgY in detection and neutralization of pathogenic Shigella strains.

Keywords: Immunoglobulin Y; Egg Yolk; Shigella flexneri; Dot ELISA; UV Spectrum



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Citation: Prakash Narayana Reddy., et al. “IgY: A Promising Alternative to IgG in Immunodiagnostics".Acta Scientific Biotechnology 1.1 (2020): 18-21.


Acceptance rate32%
Acceptance to publication20-30 days
Impact Factor1.014

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