Endale Hailu^1,2,3, Rehana Kandhro^1,2, Guodong Zhao^1,2, Tang Jian^1,2, Hao Changfu^1,2, Workneh Ayalew³, Abebe Jenberie³ and Yuehua Zhang^1,2*

¹Jiangsu University of Science and Technology, Zhenjiang, Jiangsu 212018, China
²Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang, Jiangsu 212018, China
³ICIPE Ethiopia, Addis Ababa, P.O. Box 5689, Ethiopia

*Corresponding Author: Yuehua Zhang, University of Science and Technology, Zhenjiang Jiangsu 212018, China.

Received: April 02, 2026; Published: April 23, 2026

Abstract

The present work was carried out to investigate quantification of Ace2 gene expression that was obtained from midgut, fat body, malpighain tubule, head and haemolymph tissues of Philosamia cynthia ricini which was exposed to micro-amounts of pyrethroid (0.002 mg/L fenvalerate) insecticides. Actin3 used as internal control gene. A qPCR was employed and data were analyzed by excel version 16; the target gene was amplified by PCR and inserted into pET28a vector; recombinant plasmid DNA was transformed into E. coli BL21 competent cells; the hypothesized colonies were chosen on LB plate containing Kanamycin and confirmed by PCR, restriction enzyme digestion and sequencing analysis. The verified recombinant pET28a-Pcrace2 plasmid DNA was expressed through inducer (IPTG) for 24 h and protein was subjected for SDS-PAGE analysis. These experiments were done in June 2018 to March, 2019 at Sericultural Research Institute, Zhenjiang, China. Results showed that expression of Pcrace2 gene can be increased due to fenvalerate exposure in all tissues under investigation, except at 9 hrs in FB and 3 hrs in haemolymph. 1485bp of Pcrace2 gene CDS with 494 Amino acid sequences was successfully amplified; its phylogeny tree analysis revealed that Pcrace2 gene has closely related to Ace 2 gene of B. Mori with accession number ABY50089.1; the partial (630bp) CDS of Pcrace2 sequence was cloned and 23.1kbp protein was induced using 7.5 mM IPTG and 5.0 mMIPTG but control sample did not. Two concentration band sizes of inducer were similar which desires future work and relative expression level in FB tissue and the ORF of the gene requires further work as well.

Keywords: Ace 2; Cloning; Philosamia cynthia ricini; Prokaryotic Expression; qPCR

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Citation

Citation: Endale., et al. “Quantitative Analysis of Philosamia cynthia ricini Ace 2 (Pcrace2) Gene at Transcriptional Level and Prokaryotic Expression". Acta Scientific Agriculture 10.5 (2026): 58

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Copyright: © 2026 Endale., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.