Milan A Bhatt*, Kathad H Kamliya and Nana K Bhammar

Ashirwad Nutrients, Mahuva, Bhavnagar, Gujarat, India

*Corresponding Author: Milan A Bhatt, Ashirwad Nutrients, Mahuva, Bhavnagar, Gujarat, India.

Received: January 07, 2026; Published: January 18, 2026

Abstract

This study investigated the impact of BioTriNova, which incorporates green seaweed extract, mycorrhizal fungi, and humic substances on the initial growth performance of sugarcane under controlled field conditions. A Completely Randomized Design with two treatments, BioTriNova and a non-treated control was used, and observations were recorded at 15, 30, 45, 60, and 75 days after sowing. Treated plants showed higher values for plant height, root length, and root biomass than the control throughout the study. In the treatment group, plant height varied from 38.14 to 88.00 cm, whereas in the control group, it only reached 28.45 to 69.40 cm. Root length increased substantially during treatment, ranging from 19.43 to 63.62 cm, contrary to 11.14 to 44.23 cm in the control. Root biomass of BioTriNova-treated plants compared with from 0.21 to 1.34 g, surpassing the control range of 0.07 to 1.00 g. The improvements are increased to the combined action of seaweed-derived bioactive substances that encourage shoot and root initiation, humic substances that improve nutrient mobility and soil activity, and mycorrhizal fungi that increase nutrient and water absorption. The results show that the integrated formulation provides greater development benefits than individual bio-stimulants reported in earlier studies. Consequently, BioTriNova emphasizes strong potential as a sustainable alternative for enhancing early sugarcane development and decreasing chemical fertilizer input.

 Keywords: Sugarcane; BioTriNova; Seaweed Extract; Humic Substances; Mycorrhizal Fungi; Biostimulant; Root Development; Sustainable Agriculture

References

1. Ali Sara E., et al. “More than sweet: A phytochemical and pharmacological review of sugarcane (Saccharum officinarum L.)”. Food Bioscience 44 (2021): 101431.
2. da Silva Vinicius S G., et al. “Nutritional requirement of sugarcane cultivars”. Journal of Agricultural Science 4 (2018).
3. Kurniawati Ari., et al. “Opportunities and challenges of bio-based fertilizers utilization for improving soil health”. Organic Agriculture 3 (2023): 335-350.
4. Nakachew Kelemu., et al. “Review on enhancing the efficiency of fertilizer utilization: Strategies for optimal nutrient management”. Open Agriculture1 (2024).
5. Bisht Nikita and Puneet Singh Chauhan. “Excessive and disproportionate use of chemicals cause soil contamination and nutritional stress”. Soil Contamination. IntechOpen (2020).
6. Sun W and MH Shahrajabian. “The application of arbuscular mycorrhizal fungi as microbial biostimulant”. Plants 17 (2023): 3101.
7. Ruslan Fatin Shazwani., et al. “Bioactive compounds… of green seaweed species (Chlorophyta)”. Squalen Bulletin 1 (2021): 41-55.
8. Ali Omar., et al. “Biostimulant properties of seaweed extracts in plants”. Plants 3 (2021): 531.
9. Kora Aruna Jyothi. “Commercial bacterial and fungal microbial biostimulants used for agriculture in India”. Microbial Biostimulants (2022): 159-175.
10. Jacomassi Lucas Moraes., et al. “A seaweed extract-based biostimulant mitigates drought stress in sugarcane”. Frontiers in Plant Science 13 (2022): 865291.
11. Kłeczek Aneta. “Agricultural use of natural biostimulants-humic substances: a review”. Rocznik Ochrona Środowiska 24 (2022).
12. Gerke Jörg. “Concepts and misconceptions of humic substances…”. Agronomy5 (2018): 76.
13. Mirbakhsh Mandana and Zahra Zahed. “Enhancing phosphorus uptake in sugarcane…” arXiv (2023).
14. da Silva Maura Santos Reis de Andrade., et al. “Humic substances + PGPR…” Frontiers in Microbiology 12 (2021): 719653.
15. Zhao Yonglong., et al. “Enhancing sugarcane growth… network-structured fertilizer synergist”. Sustainability2 (2023): 1428.
16. Claassens Anders. “Mycorrhizal gateways”. Dissertation, Southern Cross University (2024).
17. Mistry Jigar T., et al. “Influence of Commercial AMF on Sugarcane”. Technology 44 (2023): 3971-3977.
18. Spinoso-Castillo José Luis., et al. “AMF improves ex vitro acclimatization…”. Plants 3 (2023): 687.
19. Iftikhar Anwaar., et al. “Role of phosphate solubilizing bacteria…”. Journal of Xi’an Shiyou University 9 (2023).
20. Gumiere Thiago., et al. “Phosphorus source driving soil microbial interactions…”. Scientific Reports 9 (2019): 4400.
21. Sun Wenli and Mohamad Hesam Shahrajabian. “AMF as microbial biostimulant…”. Plants17 (2023): 3101.
22. Ali Omar., et al. “Biostimulant properties of seaweed extracts…”. Plants 10.3 (2021): 531.
23. Rani DV., et al. “Nanobiostimulants…”. Nanobiostimulants (2024): 457-475.
24. Zhao Yonglong., et al. “Enhancing sugarcane growth…”. Sustainability2 (2023): 1428.
25. Tripathi Sachchidanand., et al. “Influence of synthetic fertilizers…”. Agrochemicals Detection (2020): 25-54.
26. Sible Connor N., et al. “Plant biostimulants: A categorical review…”. Agronomy7 (2021): 1297.
27. Sanghera Gulzar S. “Strategies to enhance input use efficiency…”. IJCMAS 06 (2021): 774-801.
28. Nanda S., et al. “Seaweed-based biostimulants…”. IJEST12 (2022): 12839-12852.
29. Wahyuni S., et al. “Biostimulant consortium to increase sugarcane growth…” IOP Conf. Ser.: Earth Environ. Sci. 1306 (2024).
30. El-Shazly Mona M. “Effect of mycorrhizae and biostimulants on canola…”. (2020): 8303-8314.
31. Uz Zaman Qamar., et al. “Synergistic effect of AMF, biochar & seaweed…”. Industrial Crops and Products 224 (2025): 120358.
32. Leite Jose M., et al. “Co-addition of humic substances with urea…”. Heliyon10 (2020).
33. Gabriel-Hernández Magdiel, et al. “Organic biostimulants for vegetative growth of sugarcane…”. Agro Productividad (2025).

Citation

Citation: Milan A Bhatt., et al. “Effect of BioTriNova on Growth, Yield, and Productivity of Sugarcane"Acta Scientific Agriculture 10.2 (2026): 03-09.

Copyright

Copyright: © 2026 Milan A Bhatt., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.