Archana^1,3*, Preetam Verma² and N Pandey¹

¹Department of Botany, Plant nutrition and stress physiology laboratory, University of Lucknow, Lucknow, India
²Department of Biotechnology, Government polytechnic Ayodhya, India
³Chhatrapati Shivaji Degree College, Sahsoan, Prayagraj, India

*Corresponding Author: Archana, Department of Botany, Plant nutrition and stress physiology laboratory, University of Lucknow, Lucknow, India.

Received: June 12, 2025 Published: June 28, 2025

Abstract

To detect the boron deficiency induced changes in germination capacity and seed quality an experiment was conducted on soybean (Glycine max var. JS-335) plants. For this soybean plants were grown till maturity at deficient (0.033 mg L-1) and sufficient (0.33 mg L-1 ) boron supply. Seed yield was determined at 112d when plants were harvested. To study the effect of low boron concentration on seed quality- concentration of carbohydrates (sugars and starch), phenols, proteins and oil content were determined in the post harvested seeds of soybean. Membrane permeability was determined by the electrical conductivity and MDA content in seeds. Antioxidant responses of seeds were studied by estimating the parameters of CAT, PPO and POD enzymes.

Keywords: CAT; Boron Stress; Electrolyte Leakage; POD; PPO; Soybean Seed

References

1. Sacks FM. “Soy protein, isoflavones, and cardiovascular health: an American Heart Association Science Advisory for professionals from the Nutrition Committee”. Circulation7 (2006): 1034-1044.
2. Chatterjee C and Nautiyal N. “Developmental aberrations in seeds of boron deficient sunflower and recovery”. Journal of Plant Nutrition 23 (2000): 835-841.
3. Bewley DJ and Black M. “Seeds: Physiology of Development and Germination. Ed. 2^nd Springer Science and Business Media (1994).
4. Hewitt EJ. “The Essential Nutrient Element: Requirement and Interaction in Plants. In: Plant Physiology 3 F.C. Steward. (ed.), Academic Press Inc., NewYork (1963): 137-360.
5. Agarwala SC and Sharma CP. “The standardization of sand culture technique for the study of macro and micronutrient (Trace) element deficiencies under Indian conditions”. Current Science 30 (1961):
6. “International Rules for Seed Testing”. Proceedings of the International Seed Testing Association 31 (1976): 1-52.
7. Abdul Baki A and Anderson JD. “Vigour determination in soyabean seed by multiple criterion”. Crop Science 13 (1973): 630-633.
8. Nelson N. “Photometric adaptation of Somogyi’s method for determination of glucose”. The Journal of Biological Chemistry 153 (1944): 375-378.
9. Montgomery R. “Determination of glycogen”. Archives of Biochemistry and Biophysics 67 (1957): 378-386.
10. Swain T Hillis. “The phenolic constituents of Prunus domsetica The quantitative analysis of phenolic constituents”. The Journal of the Science of Food and Agriculture 10 (1959): 63-68.
11. “Official and tentative methods of analysis of the association of official agricultural chemists (11^th ed), Publ. A. O. A. C., P. O. Box 540, Benjamin Franklin Stn. Washington, 4 D. C (1965).
12. Sommour RH. “Proteins of linseed (Linum usitatissimum ) extraction and characterization by electrophoresis”. Botanical bulletin of Academia Sinica 40 (1999): 121-126.
13. Lowry OH. “Protein measurement with folin phenol reagent”. Journal of Biological Chemistry193 (1951): 265-275.
14. Heath RL and Packer L. “Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation”. Archives of Biochemistry and Biophysics 125 (1968): 189-198.
15. Sairam RK. “Effect of moisture stress on physiological activities of two contrasting wheat genotypes”. The Indian Journal of Experimental Biology 32 (1994): 593-594.
16. Euler H and Josephson K. “Uber katalase I“. Liebigs Annalen 452 (1927): 158-187.
17. Luck M. “Peroxidase In: Methods of Enzymic Analysis (Bergmeyer, H. V. Ed.), Academic Press Inc. New York (1963): 895-897.
18. Beauchamp C and Fridovich I. “Superoxide dismutase: Improved Assays and an Assay Applicable to Acrylamide Gel”. Analytical Biochemistry 44 (1971): 276-287.
19. Pandey N and Archana. “Boron-stress induced changes in water status and stomatal morphology in Zea mays and Catharanthus roseus L”. Indian Journal of Plant Physiology 14.3 (2009): 310-314.
20. Asad A. “Boron requirements of sunflower and wheat”. Journal of Plant Nutrition 25 (2002): 885-899.
21. Agarwala SC., et al. “Variable boron supply and sugarbeet metabolism”. Proceedings of the National Academy of Sciences, India 61 (1991): 109-114.
22. Han S., et al. “CO₂ assimilation photosystem II photochemistry, carbohydrate metabolism and antioxidant system of citrus leaves in response to boron stress”. Plant Science 176 (2009): 143-153.
23. Sinha P., et al. “Boron stress influences economic yield and quality in crop species”. Indian Journal of Plant Physiology 14 (2009): 200-204.
24. Sinha P and Chatterjee C. “Influence of low boron on yield and seed quality of soybean”. Indian Journal of Agricultural Research 37 (2003): 193-198.
25. Vitale A and Bollini R. “Legume storage Proteins. In: Seed Development and Germination. Kigel, J. and G. Galli (Eds), New York: Dekker (1995): 73-95.
26. LeGall M., et al. “Effects of grinding and thermal treatments on hydrolyzing susceptibility of pea proteins (P. sativum L.)”. Journal of Agricultural and Food Chemistry 53 (2005): 3057-3064.
27. Sharma PN., et al. “Effect of variable boron supply on plant growth and certain aspects of metabolism with particular reference to hydrolases in safflower”. Acta Botanica Indica 21 (1993): 44-57.
28. Molassiotis A., et al. “Boron induced oxidative dammage and antioxidant and nucleolytic responses in shoot tips culture of apple rootstock EM9 (Malus domestiac Borkh)”. Environmental and Experimental Botany 56 (2006): 54-62.
29. Ben Miled DD., et al. “Sodium chloride effect on lipase activity in germinating seeds”. Biochemical Society Transactions 28 (2000): 899-902.
30. Podbielkowska M., et al. “Coumarins as antimitotics”. International Journal of Pharmacognosy 32 (1994): 1-12.
31. Cakmak I and Romheld V. “Boron deficiency-induced impairments of cellular functions in plants”. Plant and Soil 193 (1997): 71-84.
32. Basra SMA., et al. “Assessment of cotton seed deterioration during accelerated ageing”. Seed Science Technology 31 (2003): 531-540.
33. Copland LO and McDonald MB. “Principles of seed science and technology. Chapman and Hall, New York, USA (1995).
34. Sung JM. “Lipid peroxidation and peroxide scavenging in soyabean seeds during aging”. Plant Physiology 97 (1996): 85-89.
35. Bailly C., et al. “From intracellular signalling networks to cell death: the dual role of reactive oxygen species in seed physiology”. Comptes Rendus Biologies 331 (2008): 806-814.
36. Schmitz N., et al. “Characterization of anionic soybean (Glycine max) seed coat peroxidase”. Canadian Journal of Botany 75 (1997): 1336-1341.
37. Cella R and Carbonera D. “Peroxidases and morphogenesis”. Plant Peroxidase Newsletters (1997):
38. Guan L and Scandalios JG. “Characterization of the catalaseantioxidant defense gene Cat1 of maize, and its developmentally regulated expression in transgenic tobacco”. The Plant Journal 3 (1993): 527–536.
39. Canvin DT. “Photorespiration and CO₂ concentrating mechanisms. In Plant physiology, biochemistry and molecular biology (Ed. D. T. Dennis and D. H. Turpin), Longman Scientific and Technical, Harlow (1990): 253-273.
40. Chekuboyina RK., et al. “Impact of Germination on Biochemical and Antioxidant Enzymes of Ceiba pentandra (Kapok) Seeds”. American Journal of Plant Sciences 3 (2012): 1187-1192.

Citation

Citation: Archana. “Impact of Low Boron Treatment on Germination Capacity, Membrane Permeability and Antioxidant Machinery in Soybean Seeds". Acta Scientific Agriculture 9.7 (2025): 27-35.

Copyright

Copyright: © 2025 Archana This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.