HB Roghan¹, I Sekar²*, K Sivakumar³, M Sivaprakash³ and M Tilak³

¹PhD Scholar, Forest College and Research Institute, Tamil Nadu Agricultural University, Mettupalayam, Tamil Nadu, India
²Professor, Forest College and Research Institute, Tamil Nadu Agricultural University, Mettupalayam, Tamil Nadu, India
³Associate, Professor, Forest College and Research Institute, Tamil Nadu Agricultural University, Mettupalayam, Tamil Nadu, India

*Corresponding Author: I Sekar, Professor, Forest College and Research Institute, Tamil Nadu Agricultural University, Mettupalayam, Tamil Nadu, India.

Received: January 08, 2025; Published: January 20, 2025

Abstract

Simarouba glauca predominantly known for its role in oil seed production which is considered as a oil tree with versatile and multipurpose nature that holds immense potential in agroforestry and environmental conservation. Using shoot tips from seedlings grown on Murashige and Skoog (MS) media supplemented with different doses of cytokinins and auxins, the study explores the in vitro propagation of Simarouba glauca. The strongest culture response (80.75%) for shoot induction was obtained using MS media with 3.0 mg L⁻¹ 6-benzylaminopurine (BAP) and 2.0 mg L⁻¹ kinetin; on the other hand, the combination of 3.0 mg L⁻¹ BAP and 3.0 mg L⁻¹ kinetin produced the greatest number of shoots per explant (4.38). With an average of 4.68 roots per shoot and the best rooting effectiveness (34.50%), indole-3-butyric acid (IBA) at 2.0 mg L⁻¹ was the most effective auxin. The plantlets were successfully acclimatized in a controlled environment and are being transferred to the field. The findings demonstrate the potential for large-scale propagation of Simarouba glauca to enhance plantation establishment, ecological restoration, and rural livelihoods. This study aims to establish efficient in vitro propagation techniques for Simarouba glauca, focusing on direct organogenesis from nodal segments. The ultimate goal is to facilitate large-scale production of planting material to support widespread cultivation. By addressing propagation challenges, this research contributes to the development of sustainable Simarouba glauca plantations, enhancing ecological restoration, increasing oil production, and improving rural livelihoods.

Keywords: Simarouba Glauca; In Vitro Propagation; Ecological Restoration; Agroforestry

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Citation

Citation: I Sekar., et al. “Efficient In vitro Propagation Protocol for Simarouba glauca: Enhancing Agroforestry, Ecological Restoration, and Sustainable Rural Development". Acta Scientific Agriculture 9.2 (2025): 35-40.

Copyright

Copyright: © 2025 I Sekar., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.