Yerra Pavani¹*, AK Upadhyay² and B Sachidanand²

¹Department of Soil Science and Agricultural Chemistry, Tamil Nadu Agricultural University, Coimbatore, India
²Department of Soil Science and Agricultural Chemistry, College of Agriculture, JNKVV, Jabalpur, India

*Corresponding Author: Yerra Pavani, Department of Soil Science and Agricultural Chemistry, Tamil Nadu Agricultural University, Coimbatore, India.

Received: February 29, 2024; Published: April 16, 2024

Abstract

The investigation entitled “Effect of foliar spray of Pseudomonas fluorescens on BNF, nodulation attributes, Leghemoglobin content and population of P. fluorescens on phyllosphere of soybean under STCR approach” was carried out at the Research Farm of Jawaharlal Nehru Krishi Vishwa Vidyalaya, Jabalpur (India), taking soybean (variety JS 95-60). The experiment was laid out under randomized block design with 4 replications of 10 treatments based on targeted yields which consisted of control; T₂= control + foliar spray of P. fluorescens; T₃ = General Recommended Dose (20:60:20 kg N, P₂O₅ and K₂O ha^-1); T₄ = General Recommended Dose + foliar spray of P. fluorescens; T₅ = 23:62:27 kg N, P₂O₅ and K₂O ha^-1 (Targeted Yield 25 q ha^-1); T₆ = 23:62:27 kg N, P₂O₅ and K₂O ha^-1 (Targeted Yield 25 q ha^-1) + foliar spray of P. fluorescens; T₇= 49:88:47 kg N, P₂O₅ and K₂O ha^-1 (Targeted Yield 30 q ha-¹); T₈ = 49:88:47 kg N, P₂O₅ and K₂O ha^-1 (Targeted Yield 30 q ha^-1); T₉ = 73:114:66 kg N, P₂O₅ and K₂O ha^-1 (Targeted Yield 35 q ha^-1); T₁₀ = 73:114:66 kg N, P₂O₅ and K₂O ha^-1 (Targeted Yield 35 q ha^-1) + foliar spray of P. fluorescens. Regarding the effect of foliar spray of P. fluorescens as influenced by different NPK levels results obtained on treatment T₆ (23:62:27 kg N, P₂O₅ and K₂O ha^-1 + foliar spray P. fluorescens) has significantly increased nodule fresh weight (1.32 g), nodule dry weight (0.66 g), leghaemoglobin content (2.37mM at 45DAS) and maximum amount of BNF (8 kg ha^-1) when compared to nutrient applied at higher doses. The increase in population of Pseudomonas could be attributed to increased availability of N, P and K through applied fertilizers

Keywords: Pseudomonas fluorescens; Targeted Yield; Nutrient Management; Sustainable Agriculture

References

1. FAOSTAT Statistical Database. Food and Agriculture Organization of the United Nations (2020).
2. Thakare K G., et al. "Influence of nutrients and hormones on biochemical and yield and yield contributing parameters of soybean”. Journal of Soils and Crops 1 (2006): 210-216.
3. Barik K C and A S Chandel. "Effect of copper fertilization on plant growth, seed yield, copper and phosphorus uptake in soybean (Glycine max) and their residual availability in Mollisol”. Indian Journal of Agronomy2 (2001): 319-326.
4. Vance Carroll P. "Enhanced agricultural sustainability through biological nitrogen fixation”. Biological fixation of nitrogen for ecology and sustainable agriculture. Berlin, Heidelberg: Springer Berlin Heidelberg, (1997):179-186.
5. Rana Anuj., et al. "Biofortification of wheat through inoculation of plant growth promoting rhizobacteria and cyanobacteria”. European Journal of Soil Biology 50 (2012): 118-126.
6. Rastogi Gurdeep., et al. "New insights into the structure and function of phyllosphere microbiota through high-throughput molecular approaches”. FEMS Microbiology Letters1 (2013): 1-10.
7. Hoffland Ellis., et al. "Comparison of systemic resistance induced by avirulent and nonpathogenic Pseudomonas species”. Phytopathology7 (1996): 757-762.
8. O'sullivan D J and O'Gara F. “Traits of fluorescent Pseudomonas spp. involved in suppression of plant root pathogens”. Microbiological Reviews4 (1992): 662-676.
9. Bhattacharyya P N and Jha D K. “Plant Growth-Promoting Rhizobacteria (PGPR). Emergence in Agriculture”. World Journal of Microbiology and Biotechnology4 (2012): 1327-1350.
10. Sadasivam S and A Manickam. “Biochemical methods for agricultural sciences”. Wiley eastern limited, (1992).
11. Kasturikrishna S and I PS Ahlawat. "Growth and yield response of pea (Pisum sativum) to moisture stress, phosphorus, sulphur and zinc fertilizers”. (2005): 588-596.
12. Parmar N and K R Dadarwal. "Stimulation of nitrogen fixation and induction of flavonoid‐like compounds by rhizobacteria”. Journal of applied Microbiology1 (1999): 36-44.
13. Tilak K V B R., et al. "Synergistic effects of plant‐growth promoting rhizobacteria and Rhizobium on nodulation and nitrogen fixation by pigeonpea (Cajanus cajan)”. European Journal of Soil Science1 (2006): 67-71.
14. Subramanian Parthiban., et al. "Endophytic bacteria improve nodule function and plant nitrogen in soybean on co-inoculation with Bradyrhizobium japonicum MN110”. Plant Growth Regulation 76 (2015): 327-332.
15. Flores-Duarte Noris J., et al. "Role of nodulation-enhancing rhizobacteria in the promotion of Medicago sativa development in nutrient-poor soils”. Plants9 (2022): 1164.
16. Antoine Berger., et al. "Nitrate reductases and hemoglobins control nitrogen-fixing symbiosis by regulating nitric oxide accumulation”. Journal of Experimental Botany (2021).
17. Nash Dudley T and Herbert M. Schulman. "Leghemoglobins and nitrogenase activity during soybean root nodule development”. Canadian Journal of Botany24 (1976): 2790-2797.
18. Deepika C., et al. “Explicit effect of Phyllospheric Microorganism on Growth Promotion of Pearl Millet (Pennisetum glaucum)”. International Journal of Current Microbiology and Applied Sciences 3 (2017): 1046-1105.
19. Rao DLN. "Biological Nitrogen Fixation and Biofertilizers: Status and Prospects”. YP Abrol, N. raghuram, and MS Sachdev, eds. Agricultural Nitrogen Use and its Environmental Implications, New Delhi: IK Publishing House Private Limited (2007): 395-414.
20. Gabriela da Silva Freitas., et al. "Foliar Spray Inoculation with Plant Growth Promoting Bacteria Associated with Nitrogen Doses in Megathyrsus maximus cv. BRS Zuri”. Agronomy (2023).
21. Karolina A., et al. "Holistic view of biological nitrogen fixation and phosphorus mobilization in Azotobacter chroococcum NCIMB 8003”. Frontiers in Microbiology (2023).
22. Gross C M., et al. "Biological nitrogen fixation and nifH gene abundance in deadwood of 13 different tree species”. Biogeochemistry (2022).
23. Wortmann Charles S., et al. "Nitrogen response of grain sorghum in rotation with soybean”. Agronomy Journal3 (2007): 808-813.

Citation

Citation: Yerra Pavani., et al. “Effect of Foliar Spray of Pseudomonas fluorescens on BNF, Nodulation Attributes, Leghemoglobin Content and Population of P. fluorescens on Phyllosphere of Soybean Under STCR Approach". Acta Scientific Agriculture 8.5 (2024): 29-35.

Copyright

Copyright: © 2024 Yerra Pavani., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.