Acta Scientific Agriculture (ASAG)(ISSN: 2581-365X)

Research Article Volume 8 Issue 5

Effect of Three Different Fig tree Leaf Powder and Vitamin C Supplemented Diet on Serum Biochemistry and Haematological Parameters of Laying Hens

Omoniyi IS*, Adu OA, Osowe CO, Oloruntola OD, Jimoh OA and Onibi GE

Department of Animal Production and Health, The Federal University of Technology, Akure, Nigeria

*Corresponding Author: Omoniyi IS, Department of Animal Production and Health, The Federal University of Technology, Akure, Nigeria.

Received: January 23, 2024; Published: April 11, 2024


This study was conducted to investigate the physiology of laying hen fed Ficus carica, Ficus exasperate and Ficus thonningii leaf powder and vitamin C supplemented diet.
One hundred and forty four (144) - 28weeks old Lohnman brown laying birds were randomly allocated into eight (8) treatments, with three (3) replicates of six (6) birds per replicate. The birds were fed same diet with different supplementation; diet 1: no supplementation; diet 2: 200mg/kg vitamin C supplementation; diet 3: 1.0 g/1kg Ficus carica supplementation; diet 4: 1g/1kg Ficus exasperata supplementation; diet 5: 1g/1kg Ficus thonningii supplementation; diet 6: 200 mg/kg vitamin C + 1g/1kg Ficus carica supplementation; diet 7: 200mg/kg vitamin C + 1g/1kg Ficus exasperata supplementation; diet 8: 200 mg/kg vitamin C + 1g/1kg Ficus thonningii supplementation respectively. At the end of the 12-weeks feeding trial, blood samples were taken from the wing vein and assayed for serum biochemistry and haematological parameters. The data obtained were subjected to one way ANOVA.
The result showed that Alanine transaminase (93.45 – 106.96 U/L) and serum creatinine (0.91 – 1.33mg/dl) of laying birds fed the treatment diets were significantly (p<0.05) lowered than birds on diet 1 107.47u/l and 1.38mg/dl respectively. Blood glucose of laying birds fed diet 3, diet 4 and diet 5 (6.74, 6.65 and 6.95mmol/l) are significantly (p<0.05) reduced compare to the other diet. Cholesterol level of laying birds fed, diets 5 – 8 (0.62, 0.57, 0.87 and 0.87) reduced significantly (p<0.05) than diet 1 (2.67mmol/l). Superoxide dismutase of laying birds fed treatment diet (56.79 – 70.43%) was significantly higher than diet 1 (49.08%). Lipid peroxidation of laying birds fed diets 2-8 were (0.03, 0.03, 0.09, 0.04, 0.09, 0.24, 0.08TBARS/mg protein respectively) were significantly lowered than diet 1 (0.85TBARS/mg protein). Packed cell volume of birds fed with diet 2 (36%) were significantly (p < 0.05) higher than the rest diet. Red blood cell of birds fed diet 5 (4.00 × 106/L) were significantly (p < 0.05) higher than the other diets which had similar value. The Lymphocyte of birds fed diet 3 (76.00 × 109/L) were significantly (p < 0.05) higher than diet 1 (58.00 × 109/L). In conclusion, the antioxidant enzymes, blood profile of the laying birds were all improved by the supplementation of 200 mg/kg vitamin C. The blood enzyme concentration and biochemical indices of the laying birds were suppressed by the combination of 200mg/kg of vitamin C and 1g/kg of Ficus exasperata.

Keywords: Ficus Carica; Ficus Exasperate; Ficus Thonningii; Vitamin C. Haematology; Serum Biochemistry


  1. “Poultry Sector Study in Nigeria”. Sustainable, Agricultural Innovative International Publication of Netherlands Enterprise Agency (2020).
  2. ASL 2050. “Africa Sustainable Livestock 2050”. Livestock and livelihoods spotlight Nigeria Cattle and Poultry sectors. Food and Agriculture Organization of the United Nations. Date assessed (2022).
  3. Jane B. “Africa: Poultry and eggs are the winning proteins, with demand for feed and genetics expected                       to                                     rise                    sharply (2020).
  4. Govindasamy P., et al. “Early nutrition and its importance in poultry: A review”. Indian Journal of Animal Nutrition 3 (2016): 245-252.
  5. Salako Y. “Assessment of packed cell volume among students of Federal College of Veterinary and Medical Laboratory Technology, Vom, Plateau State”. International Journal of Advanced Research05 (2020): 457-460.
  6. Isaac L., et al. “Hematological properties of different breeds and sexes of rabbits”. Proceedings the 18th Annual Conference of Animal Science Association of Nigeria (2013): 24-27.
  7. Zhang SBoY., et al. “Assessment of Hematologic and Biochemical Parameters for Healthy Commercial Pigs in China”. Animals (Basel) 18 (2022): 2464.
  8. Nawaz H., et al. “Phytochemical composition, antioxidant potential, and medicinal significance of Ficus”. Intech Open (2019).
  9. Taiwo IA., et al. “Glycaemic activity of ficus exasperata in fructose-induced glucose intolerance in rats”. Researcher 2 (2010): 80-83.
  10. Feng C and Ma Y. “Isolation and anti-phytopathogenic activity of secondary metabolites from Alternaria sp. FL25, an endophytic fungus in Ficus carica”. Chinese Journal of Applied and Environmental Biology 16 (2010): 76-78.
  11. Ahmadu S., et al. “An overview of Vitamin C as an antistress in poultry”. Malaysian Journal of Veterinary Research 2 (2016): 9-22.
  12. Zahra N., et al. “Does Vitamin C supplementation exert profitable effects on serum lipid profile in patients with type 2 diabetes? A systematic review and dose-response meta-analysis”. Pharmacological Research 169 (2021): 1043-6618.
  13. Afrose S., et al. “Effect of dietary karaya saponin on serum and egg yolk cholesterol in laying hens”. British Poultry Science 51.6 (2010): 797-804.
  14. Adebayo , et al. “The Performance and Haematological Indices ofBroiler Chickens Fed Chromium Picolinate, and Vitamin C Supplemented Diets”. Asian Journal of Research in Animal and Veterinary Science 6.4 (2020): 54-61.
  15. Adebayo FB., et al. “Egg qualities and haematological indices of laying birds fed chromium picolinate and Vitamin C supplemented diets”. Annals of Animal and Biological Research1 (2021): 10-16.
  16. Mohiti-Asli M and Zaghari M. “Does dietary vitamin E or C decrease egg yolk cholesterol”. Biological Trace Element Research 138 (2010): 60-68.
  17. Oloruntola OD., et al. “Effect of feeding broiler chicken with diets containing Alchornea cordifolia leaf meal and enzyme supplementation”. Arch de Zootec 252 (2016): 489-498.
  18. Jimoh OA., et al. “Oxidative stability and spermatozoa kinetics of cock semen in pineapple juice based diluent”. Livestock Research for Rural Development108 (2020).
  19. “Statistical Analysis Systems Institute. Version 9.1, SAS Institute Inc., Cary, North Carolina, USA (2008).
  20. Osowe CO., et al. “The performance, haematological indices and serum chemistry of two breeds of broiler chickens fed Ficus exasperata leaf powder and vitamin C supplemented diets”. Cuban Journal of Agricultural Science 4 (2022).
  21. Zhang GM., et al. “Reference intervals for total bilirubin, alanine amino transferase, aspartate amino transferase and creatinine in healthy Chinese medical science monitor”. International Medical Journal of Experimental and Clinical Research 20.177 (2021): 8-1782.
  22. Oyewole OI and Oladele JO. “Changes in activities of tissues enzymes in rats administered Ficus exasperata leaf extract”. International Journal of Biological and Chemical Sciences 1 (2017): 378-386.
  23. Xu F., et al. “The Potential Use of Vitamin C to Prevent Kidney Injury in Patients with COVID-19”. Diseases (Basel, Switzerland) 9.3 (2021): 46.
  24. Bradley J McEwen. “Impact of cardiometabolic disease on cognitive function”. Nutraceuticals in Brain Health and Beyond (2021): 357-
  25. Joseph B and Raj SJ. “Phytopharmacological and phytochemical properties of three Ficus species - An overview”. International Journal of Pharmacology and Biological Sciences 1 (2010): 246-253.
  26. Kha RU., et al. “Effect of Vitamin E in heat-stressed poultry”. World’s Poultry Science Journal; 67 (2011): 469-478.
  27. Osowe CO., et al. “Proximate and mineral composition, phytochemical analysis, and antioxidant activity of fig trees (Ficus spp.) leaf powder”. Asian Journal of Biochemistry, Genetics and Molecular Biology 1 (2021): 19-29.
  28. Kurniawan MF and Audita M. “Formulation and evaluation of physical properties, anti-cholesterol activities from Ficas carica L. leaves extract tablet”. Science and Technology lndonesia: 6.4 (2021): 2580-4405.
  29. Ahur VM., et al. “Hypolipidemic Activity of the Methanolic Extract of Ficus thonningii (Blume) Leaves in Hypercholesterolemic-Induced Rats”. Nigerian Veterinary Journal2 (2014): 956-967.
  30. Keti Z., et al. “Flavonoids and Their Metabolites: Prevention in Cardiovascular Diseases and Diabetes”. National of Library of Medicine 3 (2017): 19.
  31. Younus H. “Therapeutic potentials of superoxide dismutase”. International Journal of Health Sciences 3 (2018): 88-93.
  32. Muralikrishnan G., et al. “Protective effects of Ocimum sanctum on lipid peroxidation and antioxidant status in streptozocin-induced diabetic rats”. Natural Product Research 26.5 (2012): 474-478.
  33. Alvin S. “Introduction to haematology”. Concise Guide to hematology (2019): 1-
  34. Etim N., et al. “Haematological parameters and factors affecting their values”. Journal Agricultural Science 1 (2019): 37-47.
  35. Chidozie VN., et al. “Determination of Saponin Content of Various Parts of Six Citrus species”. International Research Journal of Pure and Applied Chemistry1 (2014): 137-143.
  36. Odo GE., et al. “Toxicity and effects of fig (Ficus carica) leaf aqueous extract on haematology and some biochemical indices of wistar albino rats (Rattus norvegicus)”. Journal of Medicinal Plants Research 22 (2016): 298-305.
  37. Bushra FH. “Effect of water extract of ficus carica leaves on hematological, biochemical parameters and on histology of pancreas, liver and kidney of dexamethasone induced diabetic male rabbits”. Life Science Archives 6 (2016): 798 to 806.
  38. Zhongyuan L., et al. “A comprehensive review on phytochemistry, bioactivities, toxicity studies, and clinical studies on ficus carica leaves”. Biomedicine and Pharmacotherapy 137 (2021): 111393.
  39. Poku PA., et al. “Effect of vitamin C supplementation on keet mortality, growth performance and blood profile of indigenous Guinea fowl (Numida meleagris) in Ghana”. Livestock Research for Rural Development 11 (2018).
  40. Opoola E., et al. “Effect of Vitamin C supplementation on haematological and serum biochemical indices of laying hens diagnosed with fowl typhoid under tropical environment”. Nigeria Journal of Animal Science3 (2018).
  41. Dangarembizi R., et al. “Phytochemistry, Pharmacology and Ethnomedicinal uses of Ficus thonningi (Blume moraceae): A Review”. African Journal of Traditional Complement Alternative Medicine 2 (2013): 203-212.
  42. David OK., et al. “Effects of resveratrol on cerebral blood flow variables and cognitive performance in humans: a double-blind, placebo-controlled, crossover investigation”. The American Journal of Clinical Nutrition 6 (2010): 1590-1597.
  43. Bozonet SM and Carr AC. “The Role of Physiological Vitamin C Concentrations on Key Functions of Neutrophils Isolated from Healthy Individuals”. Nutrients6 (2014): 1363.
  44. Imran M., et al. “Chemical composition and biological studies of Ficus benjamina”. Chemistry Central Journal 1 (2014): 12.


Citation: Omoniyi IS., et al. “Effect of Three Different Fig tree Leaf Powder and Vitamin C Supplemented Diet on Serum Biochemistry and Haematological Parameters of Laying Hens". Acta Scientific Agriculture 8.5 (2024): 10-18.


Copyright: © 2024 Zdioruk N., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


Acceptance rate32%
Acceptance to publication20-30 days
Impact Factor1.014

Indexed In

News and Events

  • Certification for Review
    Acta Scientific certifies the Editors/reviewers for their review done towards the assigned articles of the respective journals.
  • Submission Timeline for Upcoming Issue
    The last date for submission of articles for regular Issues is July 30, 2024.
  • Publication Certificate
    Authors will be issued a "Publication Certificate" as a mark of appreciation for publishing their work.
  • Best Article of the Issue
    The Editors will elect one Best Article after each issue release. The authors of this article will be provided with a certificate of "Best Article of the Issue"
  • Welcoming Article Submission
    Acta Scientific delightfully welcomes active researchers for submission of articles towards the upcoming issue of respective journals.

Contact US