Acta Scientific Agriculture (ASAG)(ISSN: 2581-365X)

Research Article Volume 7 Issue 12

Molecular Assessment of Cultivar Diversity for Water Deficit Stress Tolerance in Saccharum Officinarum using SSR Markers

Shoukat Jabeen1*, Devarajan Thangadurai1, Rayappa Bharamappa Khandagave2, Sreenivasa Venkatarayappa3, Mahadevaswamy H Kumaraswamy3, Rakesh Tawadare1, Maruti Ambiger1 Ravichandra Hospet1 and Pavitra Chippalakatti1

1Department of Botany, Karnatak University, Dharwad, Karnataka, India
2S. Nijalingappa Sugar Institute, Belagavi, Karnataka, India
3ICAR-Sugarcane Breeding Institute, Coimbatore, Tamil Nadu, India

*Corresponding Author: Shoukat Jabeen, Department of Botany, Karnatak University, Dharwad, Karnataka, India.

Received: October 30, 2023; Published: November 06, 2023

Abstract

Eight SSR markers were utilized for assessing the genetic diversity among the 23 sugarcane cultivars. Based on the grouping pattern, the genetic relatedness among sugarcane cultivars was identified. UPGMA based dendrogram analysis grouped 23 sugarcane cultivars into two different clusters. Cluster I consist of two groups. Group I has four cultivars Co 09004, Co 14011, Co 95020 and Co 13003, which were the good cultivars with respect to water stress tolerance. Subsequently, group II has both good cultivar Co 93009 and moderately susceptible Co 0303, Co 92013, Co 12007 and Co 98017 cultivars. Cluster II has been bifurcated into Group III, and group IV. Group III consist of sensitive cultivars Co 92020, Co 13006, Co 86032, Co 92002 and Co 90003, moderately susceptible cultivar Co 85019 Co 98008, Co 94005, Co 08020 and Co 05001 as tolerant cultivar. Subsequently, group IV has both tolerant cultivars Co 10033 and CoC 671and sensitive cultivars Co 06015, and Co 07015. Cultivar Co 10033 and CoC 671 showed highest water stress tolerance and yield. The results revealed that they can be used as parental lines for further breeding. These parental lines inturn serve for backcrossing with good cultivars such as Co 09004, Co 14011, Co 95020 and Co 13003 to improve the genetic potential of sugarcane.

Keywords: Molecular Diversity; UPGMA; (SSR); Jaccard’s Similarity Index; Sugarcane

References

  1. Ali A., et al. “Molecular identification and genetic diversity analysis of Chinese sugarcane (Saccharum hybrids) varieties using SSR markers”. Tropical Plant Biology 10 (2017):194-203.
  2. Aljumali SJ. “Genetic diversity of aromatic rice germplasm revealed by SSR Markers”. Biomed Research International 15 (2018).
  3. Annual Report. ICAR- Sugarcane Breeding Institute, Coimbatore, India (1994): 13-14.
  4. Annual Report. ICAR- Sugarcane Breeding Institute, Coimbatore, India (2005): 14-15.
  5. Annual Report. ICAR- Sugarcane Breeding Institute, Coimbatore, India (2008): 15-16.
  6. Annual Report. ICAR- Sugarcane Breeding Institute, Coimbatore, India (2012): 17-18.
  7. Annual Report. ICAR- Sugarcane Breeding Institute, Coimbatore, India (2020): 11-12.
  8. Bower R., et al. “Transgenic sugarcane plants via microprojectile bombardment”. Plant Journal3 (1992): 409-416.
  9. Charcosset A., et al. “Use of molecular markers for the development of new cultivars and the evaluation of genetic diversity”. Euphytica1 (2004):81-94.
  10. Chen PH., et al. “SSR marker-based analysis of genetic relatedness among sugarcane cultivars (Saccharum hybrids) from breeding programs in china and other countries”. Sugar Tech 11.4 (2004): 347-354.
  11. Chidambaram K., et al. “Molecular characterization of sugarcane genotypes using SSR Markers”. International Journal of Botany and Research6 (2017): 23-28.
  12. Cox MC., et al. “Productivity traits in southern and central regions and the impact of new varieties”. Proceeding of Australian Society of Sugarcane Technology 17 (1995): 1-7.
  13. De Silva MA., et al. “Use of physiological parameters as fast tools to screen for drought tolerance in sugarcane”. Brazilian Journal of Plant Physiology3 (2007): 193-201.
  14. Dinh TH., et al. “Photosynthetic response and nitrogen use efficiency of sugarcane under drought stress conditions with different nitrogen application levels”. Plant Production Science4 (2017):412-422.
  15. Dirlewanger E., et al. “Development of microsatellite markers in peach [Prunus persica (L.) Batsch] and their use in genetic diversity analysis in peach and sweet cherry (Prunus avium)”. Theoretical and Applied Genetics 105.1 (2002): 127-138.
  16. Doyle., et al. “A Rapid DNA Isolation Procedure for Small Quantities of Fresh Leaf Tissue”. Phytochemical Bulletin 19 (1987): 11-15.
  17. Duraia AA., et al. “Identification of early and mid-late maturing sugarcane varieties for western region of Tamil Nadu”. Journal of Sugarcane Research 10 (2020): 32-42.
  18. Emanuelli F., et al. “Genetic diversity and population structure assessed by SSR and SNP markers in a large germplasm collection of grape”. BMC Plant Biology 13 (2013): 39.
  19. Ganie SA., et al. “Assessment of genetic diversity of Saltol QTL among the rice (Oryza sativa) genotypes”. Physiology and Molecular Biology of Plants 22.1 (2016): 107-114.
  20. Gomati R., et al. “Antioxidant enzymes on cell membrane integrity of sugarcane varieties differing in flooding tolerance”. Sugar Tech3 (2012): 261-265.
  21. Gomati R., et al. “Physiological Studies on ratoonability of sugarcane varieties under tropical Indian condition”. American Journal of Plant Sciences 4(2013): 247-281.
  22. Gulave C., et al. “Economic impact of sugarcane variety co-86032 on farmers economy in Maharashtra”. Indian Journal of Economics and DevelopmentSS (2020): 354-359.
  23. Hasnaoui N., et al. “Molecular genetic diversity of Punica granatum (pomegranate) as revealed by microsatellite DNA markers (SSR)”. Gene 493(2012): 105-112.
  24. Hemaprabha G., et al. “Genetic diversity and selection among drought tolerant genotypes of sugarcane using microsatellite markers”. Sugar Tech4 (2012): 327-333.
  25. Jabeen S., et al. “Biochemical and physiological responses of sugarcane cultivars against water deficit stress”. Plant Archives2 (2021): 624-631.
  26. Jabeen S., et al. “Morphological responses of sugarcane cultivars against water deficit stress in northern Karnataka region of India”. Plant Archives2(2021): 662-670.
  27. Khaled KAM., et al. “Simple Sequence Repeats (SSRs) and morphological parameters associated with drought tolerance in Sugarcane (Saccharum )”. Journal of Agricultural Chemistry and Biotechnology 8.2(2017): 85-89.
  28. Kumar RA., et al. “Physiological efficiency of sugarcane clones under water-limited condition”. Transactions of the ASABE1 (2020): 133-140.
  29. Lakshmanan P., et al. “Sugarcane biotechnology: The challenges and opportunities”. In Vitro Cellular and Development Biology-Plant 41 (2005): 345-363.
  30. Lavanya DA., et al. “Analysis of genetic diversity among high sucrose genotypes of sugarcane (Saccharum) derived from CoC 671 using sugarcane specific microsatellite markers”. Electronic Journal of Plant Breeding 1.4 (2010): 399-406.
  31. Mahadevaiah C., et al. “Delineation of genotype ´ environment interaction for identification of stable genotypes for tillering phase drought stress tolerance in sugarcane”. Scientifc Reports 11(2021):
  32. Drought tolerance: Breeding Efforts in Sugarcane. Edited M Hasanuzzaman (2021): 157-172.
  33. Manechini JRV., et al. “Unraveling the genetic structure of Brazilian commercial sugarcane cultivars through microsatellite markers”. PLoS ONE 4 (2018): e0195623.
  34. Misra V., et al. “Morphological assessment of water stressed sugarcane: A comparison of waterlogged and drought affected crop”. Saudi Journal of Biological Sciences 5 (2020): 1228-1236.
  35. Nachimuthu VV., et al. “Analysis of population structure and genetic diversity in rice germplasm using ssr markers: an initiative towards association mapping of agronomic traits in oryza sativa”. Rice30 (2015).
  36. Ngamhui N., et al. “Differentially expressed proteins in sugarcane leaves in response to water deficit stress”. Plant Omics Journal 4 (2012): 365-371.
  37. Pan YB. “Highly polymorphic microsatellite DNA markers for sugarcane germplasm evaluation and variety identity testing”. Sugar Tech 8 (2006): 246-256.
  38. Pathak AD., “All India Coordinate Research Project on Sugarcane, Project Coordinator’s Report”. ICAR, Lucknow (2019).
  39. Prasitsom C., et al. “Selection of SSR markers for drought resistant sugarcane in Thailand”. International Journal of Agricultural Technology6 (2019): 997-1010.
  40. Ren X., et al. “Genetic diversity and population structure of the major peanut (Arachis hypogaea) cultivars grown in China by SSR markers”. PLoS One (2014).
  41. Sajib AM., et al. “SSR marker-based molecular characterization and genetic diversity analysis of aromatic landraces of rice (Oryza sativa)”. Journal of Bioscience Biotechnology 1.2 (2012): 107-116.
  42. Samiullah, Khan FA., et al. “Diversity analysis of sugarcane genotypes by microsatellite (SSR) markers”. International Journal of Biotechnology and Molecular Biology Research 7 (2013): 105-110.
  43. Sandhu SK., et al. “Comparative performance of sugarcane genotypes over years and locations”. Journal of Research1-2 (2012): 1-4.
  44. Shiferaw E., et al. “Exploring the genetic diversity of Ethiopian grass pea (Lathyrus sativus) using EST-SSR markers”. Molecular Breeding 30 (2012): 789-797.
  45. Simon S., et al. “Identification of two new drought specific candidate genes in sugarcane”. Electronic Journal of Plant Breeding 4 (2010): 1164-1170.
  46. Singh RK., et al. “Evaluation of microsatellite markers for genetic diversity analysis among sugarcane species and commercial hybrids”. Australian Journal of Crop Science 2 (2010): 115-124.
  47. Singh RK., et al. “Identification of sugarcane microsatellites associated to sugar content in sugarcane and transferability to other cereal genomes”. Euphytica 182 (2011): 335-354.
  48. Tawadare R., et al. “Phenotypic characterization and genetic diversity of sugarcane varieties cultivated in northern Karnataka of India based on principal component and cluster analyses”. Brazilian Archives of Biology and Technology2 (2019).
  49. Tayade AS., et al. “Effects of spatial and genotypic variability on heat and energy use efficiency in sugarcane under tropical Indian conditions”. Journal of Crop and Weed2 (2021): 219-225.
  50. Verma H., et al. “Variability assessment for root and drought tolerance traits and genetic diversity analysis of rice germplasm using SSR markers”. Scientific Reports 9 (2019): 16513.
  51. Verma KK., et al. “Silicon alleviates drought stress of sugarcane plants by improving antioxidant responses”. Biomedical Journal of Scientific and Technical Research1 (2019).
  52. Vishwanathan R., et al. “Differential induction of chitinases and thaumatin-like proteinsin sugarcane in response to infection by Colletotrichum falcatumcausing red rot disease”. Journal of Plant Diseases and Protection 5 (2005): 417-425.

Citation

Citation: Shoukat Jabeen., et al. “Molecular Assessment of Cultivar Diversity for Water Deficit Stress Tolerance in Saccharum Officinarum using SSR Markers". Acta Scientific Agriculture 7.12 (2023): 09-16.

Copyright

Copyright: © 2023 Shoukat Jabeen., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.




Metrics

Acceptance rate32%
Acceptance to publication20-30 days
Impact Factor1.014

Indexed In




News and Events


Contact US





//