Acta Scientific Agriculture (ASAG)(ISSN: 2581-365X)

Research Article Volume 7 Issue 11

Insecticidal and Anti-Feeding Activities of Cymbopogon schoenanthus, Lippia multiflora, and Ocimum americanum Essential Oils Against Larvae and Pupae of Spodoptera frugiperda (Lepidoptera: Noctuidae)

Dimitri W Wangrawa1,2*, Antoine Waongo3, Fousséni Traore3, Zakaria Ilboudo1, Forde Upshur4, Dov Borovsky5, Chloé Lahondère4, Athanase Badolo1 and Antoine Sanon1

1Laboratoire d’Entomologie Fondamentale et Appliquée, Université Joseph Ki-Zerbo, Burkina Faso
2Département des Sciences de la Vie et de la Terre, Université Norbert Zongo, Koudougou, Burkina Faso
3Laboratoire Central d’Entomologie Agricole de Kamboinse (LCEA-K), Institut de l’Environnement et de Recherches Agricoles (INERA), Ouagadougou, Burkina Faso
4Department of Biochemistry, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA
5Department of Biochemistry and Molecular Genetics, University of Colorado Anschutz Medical School, Aurora, CO, USA

*Corresponding Author: Dimitri W Wangrawa, Laboratoire d’Entomologie Fondamentale et Appliquée, Université Joseph Ki-Zerbo, Burkina Faso and Département des Sciences de la Vie et de la Terre, Université Norbert Zongo, Koudougou, Burkina Faso.

Received: September 06, 2023; Published: October 22, 2023


The Fall Armyworm (FAW), Spodoptera frugiperda is one of the main crop pests in Burkina Faso. The growing resistance of FAW to chemical insecticides and their harmful effects on humans and the environment necessitates the search for biodegradable and eco-friendly substances. Therefore, alternative control methods, such as the use of essential oils (EOs), are important in finding new strategies for the effective management of this pest. This study aims to evaluate the biological properties of EOs extracted from leaves of C. schoenanthus, L. multiflora, and O. americanum on larvae and pupae of S. frugiperda. The activities of these oils were evaluated by topical treatment of different concentrations on L2 larvae and pupae and their effect on food intake. Topical application to L2 caused dose dependent mortality and EO of O. americanum was most effective at 72h with an LC50 of 0.3%. Application of L. multiflora EO caused mortality with a LC50 of 0.4%. EO from C. schoenanthus caused LC50 and LC90 of 0.8% and 12.5%, respectively. The oils of L. multiflora and C. schoenanthus have been shown to be most effective against pupae. L. multiflora and C. schoenanthus EOs showed significant effects within 24 h after food intake. The EOs caused inhibition of larval growth and a weight loss of 0.002% per day using 1% L. multiflora EOs and 0.003% using 2% C. schoenanthus EOs. The inhibitory effects of these oils could be exploited using integrated pest management for S. frugiperda.

Keywords: Essential oils; S. frugiperda; Mortality; Lethal Concentrations; Growth; Anti-Feeding


  1. Ali Q and A Malik. “Genetic response of growth phases for abiotic environmental stress tolerance in cereal crop plants”. Genetika 1 (2021): 419-456.
  2. Lata C and R Shivhare. “Engineering cereal crops for enhanced abiotic stress tolerance”. Proceedings of the Indian National Science Academy (2021): 1-21.
  3. DGPV, Direction Générale de la Protection des Végetaux. “Rapport général de la campagne agricole 2018-2019: Lutte contre la Chenille Légionnaire d’Automne au Burkina Fas” (2018): 14.
  4. MAAH, Présence de la chenille légionnaire au Burkina : La campagne agricole sous menace B.F. Publié le jeudi 15 juin 2017 | Sidwaya . Ouagadougou, Editor (2017).
  5. Montezano DG., et al. “Host plants of Spodoptera frugiperda (Lepidoptera: Noctuidae) in the Americas. African Entomology2 (2018): 286-300.
  6. FAO, Gestion intégrée de la chenille légionnaire d’automne sur le maïs. Un guide pour les champs-écoles des producteurs en Afrique. Rome, Italie (2018): 135.
  7. CABI, Invasive Species Compendium Datasheets-Spodoptera frugiperda (fall armyworm) (2017).
  8. Prasanna B., et al. “La chenille légionnaire d’automne en Afrique: un guide pour une lutte integree contre le ravageur”. CIMMYT (2018).
  9. Hruska AJ and F Gould. “Fall armyworm (Lepidoptera: Noctuidae) and Diatraea lineolata (Lepidoptera: Pyralidae): Impact of larval population level and temporal occurrence on maize yield in Nicaragua”. Journal of Economic Entomology2 (1997): 611-622.
  10. DGESS, Rapport Global: Résultats définitifs de la campagne agropastorale 2019-2020 et perspectives alimentaire et nutritionnelle, Ouagadougou, Burkina Faso (2020): 82.
  11. Sâmia R., et al. “Effects of aqueous extracts of Copaifera langsdorffii (Fabaceae) on the growth and reproduction of Spodoptera frugiperda (JE Smith) (Lepidoptera: Noctuidae)”. Neotropical entomology5 (2016): 580-587.
  12. Viana PA and HT Prates. “Desenvolvimento e mortalidade larval de Spodoptera frugiperda em folhas de milho tratadas com extrato aquoso de folhas de Azadirachta indica”. Bragantia1 (2003): 69-74.
  13. Lima MPLd., et al. “Manejo da lagarta-do-cartucho em milho com formulações de nim e Bacillus thuringiensis subsp. Aizawai”. Ciência Rural 39 (2009): 1215-1218.
  14. Isman MB. “Botanical insecticides, deterrents, and repellents in modern agriculture and an increasingly regulated world”. Annual Review of Entomology 51 (2006): 45-66.
  15. Negrini M., et al. “Insecticidal activity of essential oils in controlling fall armyworm, Spodoptera frugiperda”. Arquivos do Instituto Biológico (2019): 86.
  16. Nobsathian S., et al. “The insecticidal potential of Piper ribesioides (Piperales: Piperaceae) extracts and isolated allelochemicals and their impact on the detoxification enzymes of Spodoptera exigua (Lepidoptera: Noctuidae)”. Phytoparasitica (2021): 1-15.
  17. Cruz GDS., et al. “Histological and histochemical changes by clove essential oil upon the gonads of Spodoptera frugiperda (JE Smith) (Lepidoptera: Noctuidae)”. International Journal of Morphology 4 (2015): 1393-1400.
  18. de Menezes, CWG., et al. “Biocontrol potential of methyl chavicol for managing Spodoptera frugiperda (Lepidoptera: Noctuidae), an important corn pest”. Environmental Science and Pollution Research5 (2020): 5030-5041.
  19. Lourenço AM., et al. “Essential oil of Siparuna guianensis as an alternative tool for improved lepidopteran control and resistance management practices”. Scientific Reports1 (2018): 1-13.
  20. Oliveira ERd., et al. “Toxicity of Cymbopogon flexuosus essential oil and citral for Spodoptera frugiperda”. Ciência e Agrotecnologia4 (2018): 408-419.
  21. Tavares WDS., et al. “Ar-turmerone from Curcuma longa (Zingiberaceae) rhizomes and effects on Sitophilus zeamais (Coleoptera: Curculionidae) and Spodoptera frugiperda (Lepidoptera: Noctuidae)”. Industrial Crops and Products 46 (2013): 158-164.
  22. Giongo A., et al. “Toxicity of secondary metabolites from Meliaceae against Spodoptera frugiperda (JE Smith) (Lepidoptera: Noctuidae)”. Neotropical Entomology6 (2016): 725-733.
  23. Sanon A., et al. “Effects of Hyptis spicigera Lam. (Labiatae) on the behaviour and development of Callosobruchus maculatus F. (Coleoptera: Bruchidae), a pest of stored cowpeas”. International Journal of Pest Management02 (2016): 117-123.
  24. Wangrawa DW., et al. “Biological activities of four essential oils against Anopheles gambiae in Burkina Faso and their in vitro inhibition of acetylcholinesterase”. International Journal of Biological and Chemical Sciences2 (2015): 793-802.
  25. Wangrawa DW., et al. “Insecticidal Activity of Local Plants Essential Oils Against Laboratory and Field Strains of Anopheles gambiae s. l. (Diptera: Culicidae) From Burkina Faso”. Journal of Economic Entomology6 (2018): 2844-2853.
  26. Hervé M. “RVAideMemoire: Testing and Plotting Procedures for Biostatistics”. R package version (2020): 9-75.
  27. Ogle DH., et al. “FSA: Fisheries Stock Analysis”. R package version8.30 (2020).
  28. Usseglio VL., et al. “Can Essential Oils Be a Natural Alternative for the Control of Spodoptera frugiperda? A Review of Toxicity Methods and Their Modes of Action”. Plants1 (2022): 3.
  29. Maggi F and G Benelli. “Essential oils from aromatic and medicinal plants as effective weapons against mosquito vectors of public health importance”. Mosquito-borne Diseases: Implications for Public Health (2018): 69-129.
  30. Bassole I., et al. “Composition and antimicrobial activities of the leaf and flower essential oils of Lippia chevalieri and Ocimum canum from Burkina Faso”. African Journal of Biotechnology 10 (2005).
  31. Wangrawa DW., et al. “Insecticidal activity of local plants essential oils against laboratory and field strains of Anopheles gambiae sl (Diptera: Culicidae) from Burkina Faso”. Journal of economic entomology6 (2018): 2844-2853.
  32. Sneha K., et al. “Antimicrobial and Larvicidal Activities of Different Ocimum Essential Oils Extracted by Ultrasound-Assisted Hydrodistillation”. Molecules5 (2022): 1456.
  33. Soro LC., et al. “Variabilité de la composition chimique de l’huile essentielle des feuilles de Lippia multiflora cultivées en Côte d’Ivoire”. Journal of Applied Biosciences 88 (2015): 8180-8193-8180-8193.
  34. Ketoh GK., et al. “Comparative effects of Cymbopogon schoenanthus essential oil and piperitone on Callosobruchus maculatus development”. Fitoterapia 7-8 (2006): 506-510.
  35. Regnault-Roger C., et al. “Essential oils in insect control: low-risk products in a high-stakes world”. Annual Review of Entomology 57 (2019): 405-424.
  36. Ayil-Gutiérrez BA., et al. “Biological effects of natural products against Spodoptera spp”. Crop Protection 114 (2018): 195-207.
  37. Wangrawa DW., et al. “Insecticidal Activity of Local Plants Essential Oils Against Laboratory and Field Strains of Anopheles gambiae s. l. (Diptera: Culicidae) From Burkina Faso”. Journal of Economic Entomology6 (2018): 2844-2853.
  38. Weinzierl RA. “Botanical insecticides, Soaps and Oils. In: Biological and Biotechnological Control of Insect Pests N.R. JE Rechcigl, edition”. Lewis publishers, Boca Raton, New York, USA (2000).
  39. Park IK., et al. “Fumigant activity of plant essential oils and components from Schizonepeta tenuifolia against Lycoriella ingenua (Diptera: Sciaridae)”. Journal of Economic Entomology5 (2006): 1717-1721.
  40. Karr LL and JR Coats. “Insecticidal properties of d-limonene”. Journal of Pesticide Science2 (1988): 287-290.
  41. Hanif MA., et al. “Essential oils, in Essential oil research” (2019): 3-17.
  42. Traboulsi AF., et al. “Insecticidal properties of essential plant oils against the mosquito Culex pipiens molestus (Diptera: Culicidae)”. Pest Management Science5 (2002): 491-495.
  43. Silva SM., et al. “Ocimum basilicum essential oil combined with deltamethrin to improve the management of Spodoptera frugiperda”. Ciência e Agrotecnologia 41 (2017): 665-675.
  44. Akhtar Y., et al. “Effect of chemical complexity of essential oils on feeding deterrence in larvae of the cabbage looper”. Physiological Entomology1 (2021): 81-91.
  45. Wangrawa WD., et al. “Essential oils and their binary combinations have synergistic and antagonistic insecticidal properties against Anopheles gambiae sl (Diptera: Culicidae)”. Biocatalysis and Agricultural Biotechnology (2022): 102347.
  46. Lemus de la Cruz AS., et al. “Nanoemulsified Formulation of Cedrela odorata Essential Oil and Its Larvicidal Effect against Spodoptera frugiperda (JE Smith)”. Molecules9 (2022): 2975.
  47. Chi M., et al. “Cempasúchil: fuente importante de carotenoides”. Revista Ciencia y Desarrollo,. julio/agosto del 28.165 (2002): 20-25.
  48. Shadia E., et al. “Chemical composition of Ocimum americanum essential oil and its biological effects against, Agrotis ipsilon, (Lepidoptera: Noctuidae)”. Research Journal of Agriculture and Biological Sciences 6 (2007): 740-747.
  49. Baskar K., et al. “Toxic effects of Couroupita guianensis against Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae)”. Neotropical entomology1 (2011): 84-91.
  50. Sayed WAA., et al. “Increasing the efficacy of the cotton leaf worm Spodoptera littoralis nucleopolyhedrosis virus using certain essential oils”. Egyptian Journal of Biological Pest Control1 (2020): 1-7.
  51. Caballero C., et al. “Antifeedant activity and effects of terpenoids on detoxication enzymes of the beet armyworm, Spodoptera exigua (Hubner)” (2008).
  52. Villafañe E., et al. “Toxic effects of Citrus aurantium and C. limon essential oils on Spodoptera frugiperda (Lepidoptera: Noctuidae)”. Natural product communications9 (2011): 1934578X1100600941.


Citation: Dimitri W Wangrawa., et al. “Insecticidal and Anti-Feeding Activities of Cymbopogon Schoenanthus, Lippia Multiflora, and Ocimum Americanum Essential Oils Against Larvae and Pupae of Spodoptera Frugiperda (Lepidoptera: Noctuidae)". Acta Scientific Agriculture 7.11 (2023): 50-62.


Copyright: © 2023 Dimitri W Wangrawa., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


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