Acta Scientific Agriculture (ASAG)(ISSN: 2581-365X)

Research Article Volume 7 Issue 8

Evaluation of Effectiveness of Fungicides against Fusarium oxysporum f. Sp. Lentis in Laboratory

Sudikshya Devkota1, Pratima Poudel1*, Tulsi Parajuli2 and Poonam Bhatt3

1Himalayan College of Agricultural Science and Technology Kirtipur, Kathmandu, Nepal
2Agriculture and Forest University, Nepal

*Corresponding Author: Sudikshya Devkota, Himalayan College of Agricultural Science and Technology Kirtipur, Kathmandu, Nepal .

Received: May 29, 2023; Published: July 15, 2023

Abstract

Almost all types of soil have the soil-transmitted pathogen Fusarium, which significantly reduces the output of lentils. This research was carried out in Plant Pathology Division, NARC, Khumaltar to study the in-vitro efficacy of fungicides against Fusarium oxysporum f. sp. lentis using the poisoned food technique. Nine treatments with three replications were used in the experiment arranged in a complete randomized design (CRD). Eight fungicides Azoxystrobin (50% WP), Bavistin (50%WP), Carbendazim (50% WP), Chlorothalonil (75% WP), Copper oxychloride (50% WP), Nativo (75%WG), Saaf (75%WP), Propiconazole (25% WP) and Triazole (70% WP) were tested at four different concentrations (50ppm, 100 ppm, 150 ppm and 200 ppm. After 24 hours, the diameter of the fungal mycelium was measured for every 6 days until the pathogen fully covered the control plate and chemical inhibitory percentage was computed. All of the fungicides considerably reduced pathogen growth, however, Carbendazim 50%WP was particularly effective in reducing mycelial growth to zero at all concentrations, followed by Tebuconazole 50% +Trifloxystrobin 25%WG and Tilt 25%EC at 150 ppm and 200 ppm. Protector Zn 75%WP showed the least inhibition in all the concentrations after 168 hrs of incubation. With higher concentrations, the chemicals showed an increased potential to inhibit.

References

  1. Bardia PK and Rai PK. “In vitro and field evaluation of biocontrol agents and fungicides against wilt of cumin caused by Fusarium oxysporum f. sp. Cumini”. Journal of Spices and Aromatic Crops 16 (2007): 88-92.
  2. Bayaa B., et al. “Screening lentil for resistance to fusarium wilt: methodology and sources of resistance”. Euphytica 98.1/2 (1997): 69-74.
  3. Bayaa B and Erskine W. “Disease of lentils”. In: Allen DJ, Lenne JM, editors. The Pathology of Food and Pasture Legumes. Wallingford, UK: CAB International and ICRISAT (1998): 423-471.
  4. Bendre NJ and Barhate BG. “A souvenir on disease management in chickpea”. Rahuri, Maharashtra, India: M.P.K.V (1998).
  5. “FAOSTAT statistical database of the United Nations Food and Agriculture Organization (FAO). Italy: Rom (2017).
  6. Rafique K., et al. “Management of vascular wilt of lentils through host plant resistance, biological control agents, and chemicals”. Pakistan Journal of Botany 48 (2016): 2085-2092.
  7. Kumar S., et al. “Vascular Wilt Disease of Lentil: A Review”. Journal of Lentil Research 4 (2010): 1-14.
  8. Garkoti A., et al. “Management of Fusarium wilt of lentils through fungicides”. Journal of Mycology and Plant Pathology 43 (2013): 333-335.
  9. Maheshwari SK., et al. “Chemical Control of Lentil Wilt caused by Fusarium oxysporum sp. Lentis”. Annual Plant Protection Science 16.2 (2008): 419-421.
  10. Maitlo SA., et al. “Comparative efficacy of different fungicides against fusarium wilt of chickpea (Cicer arietinum)”. Pakistan Journal of Botany 6 (2014): 2305-2312.
  11. Maiti D and Sen C. “Integrated biological control of Sclerotium rolfsii with nitrogenous fertilizers and harzianum”. Indian Journal of Agricultural Sciences 55 (1985): 464-468.
  12. “Statistical information on Nepalese agriculture (Time Series Information)”. Agri- business Promotion and Statistical Division, Agri. Statistics Section, Singh Durbar Kathmandu, Nepal (2012).
  13. “Statistical information on Nepalese agriculture”. Ministry of Agricultural Development, Kathmandu, Nepal (2016).
  14. Pouralibaba HR., et al. “Histopathology of the infection on resistant and susceptible lentil accessions by two contrasting pathotypes of Fusarium oxysporumsp. lentis. European Journal of Plant Pathology 148.1 (2017): 53-63.
  15. Singh VK., et al. “Efficacy of fungicides for management of wilt disease of lentils caused by Fusarium oxysporum f sp. Lentis”. Annals of Plant Protection Sciences 18 (2010): 411-414.
  16. Wani AbH., et al. “In vitro inhibitory effect of fungicides and botanicals on mycelia growth and spore germination of Fusarium oxysporum”. Journal of Biopesticides 1 (2011).
  17. Taylor P., et al. “Lentil Diseases”. In Lentil. Springer Netherlands (2007): 291-313.
  18. Cavill GWK and Vincent JM. “The esters of 4-hydroxybenzoic acid and related compounds. Part II. Relationships between the fungistatic activity, and physical and chemical properties of the esters”. Journal of the Society of Chemical Industry 66.6 (1947): 175-182.
  19. Yadav NK., et al. “Source of resistance against Fusarium wilt and Stemphylium blight in lentils (Lens culinaris Medikus)”. International Journal of Applied Sciences and Biotechnology 1 (2017): 102-107.

Citation

Citation: Pratima Poudel., et al. “Evaluation of Effectiveness of Fungicides against Fusarium oxysporum f. Sp. Lentis in Laboratory". Acta Scientific Agriculture 7.8 (2023): 35-38.

Copyright

Copyright: © 2023 Pratima Poudel., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.




Metrics

Acceptance rate32%
Acceptance to publication20-30 days
Impact Factor1.014

Indexed In




News and Events


  • Certification for Review
    Acta Scientific certifies the Editors/reviewers for their review done towards the assigned articles of the respective journals.
  • Submission Timeline for Upcoming Issue
    The last date for submission of articles for regular Issues is May 20, 2024.
  • Publication Certificate
    Authors will be issued a "Publication Certificate" as a mark of appreciation for publishing their work.
  • Best Article of the Issue
    The Editors will elect one Best Article after each issue release. The authors of this article will be provided with a certificate of "Best Article of the Issue"
  • Welcoming Article Submission
    Acta Scientific delightfully welcomes active researchers for submission of articles towards the upcoming issue of respective journals.

Contact US





//