Evaluation of Effectiveness of Fungicides against Fusarium oxysporum f. Sp. Lentis in Laboratory
Sudikshya Devkota1, Pratima Poudel1*, Tulsi Parajuli2 and Poonam Bhatt3
1Himalayan College of Agricultural Science and Technology Kirtipur, Kathmandu, Nepal
2Agriculture and Forest University, Nepal
*Corresponding Author: Sudikshya Devkota, Himalayan College of Agricultural
Science and Technology Kirtipur, Kathmandu, Nepal
May 29, 2023; Published: July 15, 2023
Almost all types of soil have the soil-transmitted pathogen Fusarium, which significantly reduces the output of lentils. This research was carried out in Plant Pathology Division, NARC, Khumaltar to study the in-vitro efficacy of fungicides against Fusarium oxysporum f. sp. lentis using the poisoned food technique. Nine treatments with three replications were used in the experiment arranged in a complete randomized design (CRD). Eight fungicides Azoxystrobin (50% WP), Bavistin (50%WP), Carbendazim (50% WP), Chlorothalonil (75% WP), Copper oxychloride (50% WP), Nativo (75%WG), Saaf (75%WP), Propiconazole (25% WP) and Triazole (70% WP) were tested at four different concentrations (50ppm, 100 ppm, 150 ppm and 200 ppm. After 24 hours, the diameter of the fungal mycelium was measured for every 6 days until the pathogen fully covered the control plate and chemical inhibitory percentage was computed. All of the fungicides considerably reduced pathogen growth, however, Carbendazim 50%WP was particularly effective in reducing mycelial growth to zero at all concentrations, followed by Tebuconazole 50% +Trifloxystrobin 25%WG and Tilt 25%EC at 150 ppm and 200 ppm. Protector Zn 75%WP showed the least inhibition in all the concentrations after 168 hrs of incubation. With higher concentrations, the chemicals showed an increased potential to inhibit.
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