Acta Scientific Agriculture (ASAG)(ISSN: 2581-365X)

Research Article Volume 7 Issue 3

Exploration of Markers associated with Heat Stress Tolerance in Pennisetum Glaucum (L.) R. Br.

Sunil Nigombam1, Reetika Mahajan1, Avijit Tarafdar1, Albert Maibam1, Satish Kumar1, Monika Dalal1, SV Amitha CR Mithra1, Sumer Pal Singh2 and Jasdeep Chatrath Padaria1*

1ICAR-National Institute for Plant Biotechnology, Pusa Campus, New Delhi, India 2ICAR-Division of Genetics, IARI, Pusa Campus, New Delhi, India

*Corresponding Author:Jasdeep Chatrath Padaria, ICAR-National Institute for Plant Biotechnology, Pusa Campus, New Delhi, India.

Received: December 14, 2022; Published: February 20, 2023


Development of high-temperature stress-tolerant Pennisetum glaucum (L.) R. Br. genotypes are essential for growing these as an irrigated summer crop for enhanced production. Identification of molecular markers associated with heat stress tolerance is a prerequisite for developing tolerant P. glaucum through molecular breeding strategies. In the present research, 59 expressed sequence tag (EST) derived microsatellite markers [50 EST-Simple Sequence Repeats (SSR) + 9 Sequence Tagged Sites (STS)] were developed from the available heat stress-responsive transcriptome data in pearl millet and validated in 24 diverse genotypes of pearl millet. Among them, four EST-SSRs markers (15.38%) and one STS markers (16.66%) displayed polymorphism respectively. The polymorphic information content (PIC) of newly developed EST-SSRs was in the range from 0.207 to 0.662 with an average value of 0.435. Based on the five polymorphic markers, the 24 pearl millet genotypes were clustered into 5 main and 11 sub-clusters. Annotation of the five transcripts for which polymorphism was detected, was found to code for genes related to stress response and signaling pathway. The EST-SSRs and STS markers thus analyzed would augment the existing SSR marker resource and find their applications in diversity assessment and marker-aided breeding programs for genetic improvement of pearl millet.

Keywords: Pearl Millet; Abiotic Stress Tolerance; SSR Markers; Validation; Polymerase Chain Reaction


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Citation: Jasdeep Chatrath Padaria., et al. “Exploration of Markers associated with Heat Stress Tolerance in Pennisetum Glaucum (L.) R. Br. Acta Scientific Agriculture 7.3 (2023): 21-31.


Copyright: © 2023 Jasdeep Chatrath Padaria., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


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