Application of RP-HPLC Method for the Analysis of Some Pesticide Residues in Water Samples

Lenche Velkoska-Markovska¹*, Biljana Petanovska-Ilievska¹ and Aleksandar Markovski²

¹Faculty of Agricultural Sciences and Food - Skopje, Ss. Cyril and Methodius University in Skopje, Republic of North Macedonia
²Institute of Agriculture - Skopje, Ss. Cyril and Methodius University in Skopje, Republic of North Macedonia

*Corresponding Author: Lenche Velkoska-Markovska, Faculty of Agricultural Sciences and Food - Skopje, Ss. Cyril and Methodius University in Skopje, Republic of North Macedonia.

Received: September 16, 2022; Published: December 22, 2022

Abstract

A reversed-phase high-performance liquid chromatography (RP-HPLC) method for determination of some pesticide residues in different water samples has been developed and validated. The investigated pesticides belong to a different chemical classis, such as: organonitrogen, among which triazines (e.g., atrazinе), organophosphorus (e.g., malathion, fenitrothion, parathion) and phenoxycarboxylic acids (e.g., 2,4-D (2,4-dichlorophenoxy) acetic acid)), which have different chemical structures and polarities. A Purospher STAR RP-8e (30 x 4 mm, 5 μm) analytical column, mobile phase consisted of acetonitrile and water, flow rate of 1 mL/min and constant column temperature at 25 °C were used for successful separation and quantitative determination of target analytes. The UV-detection was performed at 220 nm and 270 nm. Concentration and clean-up of analytes were done by solid-phase extraction (SPE). The developed method was validated according to European Commission guidelines for pesticide residue analytical methods and specificity, selectivity, linearity, precision, accuracy, and limit of quantification (LOQ) were tested for that purpose. The obtained values for relative standard deviation of retention times and peak areas (RSD ≤ 11.26%), recoveries ranged from 95.01 - 111.20%, multiple correlation coefficients (R2 ≥ 0.99) revealed that all performance characteristics were found within acceptance criteria. The method was successfully applied for determination of target pesticide residues in different water samples.

Keywords: Method Validation; Pesticide Residues; RP-HPLC; Water Samples

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Citation

Citation: Lenche Velkoska-Markovska., et al. “Application of RP-HPLC Method for the Analysis of Some Pesticide Residues in Water Samples". Acta Scientific Agriculture 7.1 (2023): 63-69.

Copyright

Copyright: © 2023 Lenche Velkoska-Markovska., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.