Biocontrol Potential and Mode of Action of Trichoderma Against Fungal Plant Diseases
Ashim Pokhrel1*, Aakash Adhikari1, Dipiza Oli2, Bipul Paudel1, Shishir Pandit1, Bigyan GC1 and Brij Raj Raut Tharu3
1Department of Agriculture, Himalayan College of Agricultural Sciences and Technology, Kathmandu, Nepal
2Montana State University, Bozeman, USA
3Georg-August Universitat Gottingen, Gottingen, Germany
*Corresponding Author: Ashim Pokhrel, Department of Agriculture, Himalayan College of Agricultural Sciences and Technology, Kathmandu, Nepal.
August 26, 2022; Published: September 15, 2022
Plant diseases, which can result in yield losses of up to 30% and are a major contributor to low crop production, pose a danger to the sustainability of the world's food supply. As more than 60% of the bio fungicides that are now registered around the globe are derived from Trichoderma-based formulations, Trichoderma spp. is the most effective bio fungicide in modern agriculture. Trichoderma-based biological control agents (BCAs) are among the many biological controls that are applied in agriculture to manage soil-borne diseases. These BCAs are commercialized and known by several names, including growth promoters, bio-fertilizers, and bio-pesticides. Trichoderma attacked other plants' pathogenic fungi and encouraged the growth of roots and plants. It employs a variety of management strategies for plant pathogenic diseases, including antibiosis, mycoparasitism, induced host cell resistance, and competition for nutrients and space. It has been discovered that the beneficial bacteria azotobacter and rhizobium interact with Trichoderma viridae. For the effective application of bio fungicides to manage plant diseases, seed treatment, seed bio priming, seedling dipping, soil application, and wound dressing are suggested. The information on Trichoderma as a biocontrol agent, its biocontrol activity, commercial production, and its use in plant disease management programs is reviewed in this research.
Keywords: Biological Agents; Bio-Fungicides; Diseases; Nutrients
- GE Harman., et al. “Trichoderma species - Opportunistic, avirulent plant symbionts”. Nature Reviews Microbiology 1 (2004): 43-56.
- J Nawrocka and U Małolepsza. “Diversity in plant systemic resistance induced by Trichoderma”. Biological Control 2 (2013): 149-156.
- M Srivastava., et al. “Trichoderma genome to genomics: a review”. Journal of Data Mining in Genomics and Proteomics 162 (2014): 602-2153.
- J López-Bucio., et al. “Trichoderma as biostimulant: exploiting the multilevel properties of a plant beneficial fungus”. Scientia Horticulturae (Amsterdam) 196 (2015): 109-123.
- HA Contreras-Cornejo., et al. “Ecological functions of Trichoderma and their secondary metabolites in the rhizosphere: interactions with plants”. FEMS Microbiology Ecology 92.4 (2016): fiw036.
- L Hatvani., et al. “The green mould disease global threat to the cultivation of oyster mushroom (Pleurotus ostreatus): a review”. in Science and cultivation of edible and medicinal fungi: Mushroom Science XVII, Proceeding of the 17th Congress of the International Society for Mushroom Science 08 485-495.
- L Hatvani., et al. “First report of Trichoderma aggressivum f. Aggressivum green mold on Agaricus bisporus in Europe”. Plant Disease 6 (2017): 1052.
- L Kredics., et al. “Biodiversity of the genus Hypocrea/Trichoderma in different habitats”. in Biotechnology and biology of Trichoderma, Elsevier (2014): 3-24.
- M Verma., et al. “Antagonistic fungi, Trichoderma: panoply of biological control”. Biochemical Engineering Journal 37.1 (2007): 1-20.
- HB Singh., et al. “Biological control of plant diseases: current status and future prospects”. Biotechnological Applications. New Indian Publishing Agency New Delhi (2009).
- IV Grigoriev., et al. “MycoCosm portal: Gearing up for 1000 fungal genomes”. Nucleic Acids Research (2014): 42.D1.l
- G Bhandari., et al. “Effectiveness of Some Chemical and Biological Pesticides against Sitophilus zeamais (Motschulsky)” (2022).
- L Kharel Sharma., et al. “Comparative Efficacy of Biological, Botanical and Chemical Treatments Against Damping Off Disease of Tomato in Chitwan”. International Journal of Social Sciences and Management2 (2022): 67-74.
- E Sharon., et al. “Parasitism of Trichoderma on Meloidogyne javanica and role of the gelatinous matrix”. European Journal of Plant Pathology 3 (2007): 247-258.
- PAVCFVSWMRRCSLSFISSGDTVFFSMLR Marra. “Study of the three-way interaction between Trichoderma atroviride, plant and fungal pathogens by using a proteomic approach”. Current Genetics 50 (2006): 307-321.
- M Ruocco., et al. “Identification of a new biocontrol gene in Trichoderma atroviride: The role of an ABC transporter membrane pump in the interaction with different plant-pathogenic fungi”. Molecular Plant-Microbe Interactions 3 (2009): 291-301.
- L Chen., et al. “Trichoderma harzianum SQR-T037 rapidly degrades allelochemicals in rhizospheres of continuously cropped cucumbers”. Applied Microbiology and Biotechnology 5 (2011): 1653-1663.
- A Masunaka., et al. “Plant growth-promoting fungus, Trichoderma koningi suppresses isoflavonoid phytoalexin vestitol production for colonization n/in the roots of lotus japonicus”. Microbes and Environment 2 (2011): 128-134.
- G Samuels. “Growth rate/colony radius” (2004).
- W Gams and J Bissett. “Morphology and identification”. Trichoderma and Gliocladium (2002): 3-31.
- X Lin and J Heitman. “Chlamydospore formation during hyphal growth in Cryptococcus neoformans”. Eukaryotic Cells 10 (2005): 1746-1754.
- GJ Samuels and PK Hebbar. “Trichoderma: Identification and Agricultural Applications”. Trichoderma - Identification and Agricultural Application (2015).
- S Subedi., et al. “EFFECT OF ORGANIC MANURES ON GROWTH AND YIELD OF COWPEA IN CHITWAN, NEPAL”. Plant Physiology and Soil Chemistry 2.2 (2022): 54-57.
- NA Zin and NA Badaluddin. “Biological functions of Trichoderma for agriculture applications”. Annals of Agricultural Sciences 65.2 (2020): 168-178.
- MA Pandit., et al. “Major Biological Control Strategies for Plant Pathogens”. Pathogens2 (2022).
- P Sharma., et al. “Biocontrol genes from Trichoderma species: a review”. African Journal of Biotechnology 86 (2011): 19898-19907.
- AC Odebode. “Control of postharvest pathogens of fruits by culture filtrate from antagonistic fungi”. Journal of Plant Protection Research 1 (2006): 1-5.
- Ç Küçük and M Kivanç. “Isolation of Trichoderma and determination of their antifungal, biochemical and physiological features”. Turkish Journal of Biology 27.4 (2003): 247-253.
- R Hermosa., et al. “Plant-beneficial effects of Trichoderma and of its genes”. Microbiology1 (2012): 17-25.
- A Viterbo and I Chet. “TasHyd1, a new hydrophobin gene from the biocontrol agent Trichoderma asperellum, is involved in plant root colonization”. Molecular Plant Pathology 4 (2006): 249-258.
- MD Eugenia., et al. “The ThPG1 endopolygalacturonase is required for the Trichoderma harzianum-plant beneficial interaction”. Molecular Plant-Microbe Interactions 8 (2009): 1021-1031.
- DC Naseby., et al. “Effect of biocontrol strains of Trichoderma on plant growth, Pythium ultimum populations, soil microbial communities and soil enzyme activities”. Journal of Applied Microbiology 1 (2000): 161-169.
- P Tripathi., et al. “Trichoderma: a potential bioremediator for environmental clean up”. Clean Technologies and Environmental Policy 4 (2013): 541-550.
- A Viterbo., et al. “Trichoderma mitogen-activated protein kinase signaling is involved in induction of plant systemic resistance”. Applied and Environmental Microbiology 10 (2005): 6241-6246.
- L Macías-Rodríguez., et al. “Trichoderma atroviride promotes tomato development and alters the root exudation of carbohydrates, which stimulates fungal growth and the biocontrol of the phytopathogen Phytophthora cinnamomi in a tripartite interaction system”. FEMS Microbiology Ecology 9 (2018): fiy137.
- L Kredics., et al. “Molecular tools for monitoring Trichoderma in agricultural environments”. Frontiers in Microbiology 9 (2018): 1599.
- M Calin., et al. “Applications of fungal strains with keratin-degrading and plant growth promoting characteristics”. Agronomy9 (2019): 543.
- M Shoresh., et al. “Involvement of jasmonic acid/ethylene signaling pathway in the systemic resistance induced in cucumber by Trichoderma asperellum T203”. Phytopathology1 (2005): 76-84.
- J Khan., et al. “Systemic resistance induced by Trichoderma hamatum 382 in cucumber against Phytophthora crown rot and leaf blight”. Plant Disease 3 (2004): 280-286.
- KH Dolatabadi., et al. “In vitro evaluation of arbuscular mycorrhizal-like fungi and Trichoderma species against soil borne pathogens”. Journal of Agricultural Technology 1 (2011): 73-84.
- LG Hjeljord., et al. “Effect of temperature and nutrient stress on the capacity of commercial Trichoderma products to control Botrytis cinerea and Mucor piriformis in greenhouse strawberries”. Biological Control 2 (2000): 149-160.
- VP Zope., et al. “Neem cake carrier prolongs shelf life of biocontrol fungus Trichoderma viridae” (2019).
- S Yendyo., et al. “Revised evaluation of Trichoderma, Pseudomonas fluorescens and Bacillus subtilis for biological control of Ralstonia wilt of tomato”. version 3; referees: 2 approved. F1000 Research 6 (2018): 2028.
- P Susiana., et al. “The resistance of potatoes by application of Trichoderma viride antagonists fungus”. in E3S Web of Conferences73 (2014): 60.
- CP Bhandari and V Karuna. “Screening of different isolates of Trichoderma harzianum and Pseudomonas fluorescens against Fusarium moniliforme infecting maize”. Pantnagar Journal of Research 2 (2013): 243-247.
- IS Druzhinina., et al. “Trichoderma: The genomics of opportunistic success”. Nature Reviews Microbiology 10 (2011): 749-759.
- GE Harman. “Overview of Mechanisms and Uses of Trichoderma spp”. Phytopathology®2 (2006): 190-194.
- PK Mukherjee., et al. “Secondary metabolism in Trichoderma - A genomic perspective”. Microbiology1 (2012): 35-45.
- R Weindling. “Trichoderma lignorum as a parasite of other soil fungi”. Phytopathology8 (1932): 837-845.
- C Altomare., et al. “Solubilization of phosphates and micronutrients by the plant-growth-promoting and biocontrol fungus Trichoderma harzianum Rifai 1295-22”. Applied and Environmental Microbiology 7 (1999): 2926-2933.
- A Osbourn. “Secondary metabolic gene clusters: evolutionary toolkits for chemical innovation”. Trends in Genetics 10 (2010): 449-457.
- F Vinale., et al. “Trichoderma-plant-pathogen interactions”. Soil Biology and Biochemistry 1 (2008): 1-10.
- VN Ramachander Turaga. “Peptaibols: antimicrobial peptides from fungi”. in Bioactive Natural Products in Drug Discovery, Springer (2020): 713-730.
- JL Reino., et al. “Secondary metabolites from species of the biocontrol agent Trichoderma”. Phytochemistry Reviews 1 (2008): 89-123.
- PK Mukherjee., et al. “Two classes of new peptaibols are synthesized by a single non-ribosomal peptide synthetase of Trichoderma virens”. Journal of Biological Chemistry 6 (2011): 4544-4554.
- RW Dunlop., et al. “An antibiotic from Trichoderma koningii active against soilborne plant pathogens”. Journal of Natural Products 1 (1989): 67-74.
- R Singh., et al. “Oil palm genome sequence reveals divergence of interfertile species in Old and New worlds”. Nature7462 (2013): 335-339.
- F Vinale., et al. “A novel role for Trichoderma secondary metabolites in the interactions with plants”. Physiological and Molecular Plant Pathology 1-3 (2008): 80-86.
- V Ahluwalia., et al. “Comparative evaluation of two Trichoderma harzianum strains for major secondary metabolite production and antifungal activity”. Natural Product Research 10 (2015): 914-920.
- A Bargaz., et al. “Soil microbial resources for improving fertilizers efficiency in an integrated plant nutrient management system”. Frontiers in Microbiology 9 (2018): 1606.
- A Mahmood and R Kataoka. “Potential of biopriming in enhancing crop productivity and stress tolerance”. in Advances in seed priming, Springer (2018): 127-145.
- M Miethke. “Molecular strategies of microbial iron assimilation: from high-affinity complexes to cofactor assembly systems”. Metallomics1 (2013): 15-28.
- MP Srivastava., et al. “Detection of siderophore production from different cultural variables by CAS-agar plate assay”. Asian Journal of Pharmacy and Pharmacology 4 (2018): 66-69.
- H Bae., et al. “Endophytic Trichoderma isolates from tropical environments delay disease onset and induce resistance against Phytophthora capsici in hot pepper using multiple mechanisms”. Molecular Plant-Microbe Interactions 3 (2011): 336-351.
- M Hyakumachi and M Kubota. “Fungi as plant growth promoter and disease suppressor”. Fungal Biotechnology in Agricultural, Food, and Environmental Applications 21 (2004): 101-110.
- I Yedidia., et al. “Effect of Trichoderma harzianum on microelement concentrations and increased growth of cucumber plants”. Plant Soil2 (2001): 235-242.
- F Vinale., et al. “Harzianic acid: a novel siderophore from Trichoderma harzianum”. FEMS Microbiology Letters 2 (2013): 123-129.
- F Cai., et al. “Harzianolide, a novel plant growth regulator and systemic resistance elicitor from Trichoderma harzianum”. Plant Physiology and Biochemistry 73 (2013): 106-113.
- HA Contreras-Cornejo., et al. “Trichoderma virens, a plant beneficial fungus, enhances biomass production and promotes lateral root growth through an auxin-dependent mechanism in arabidopsis”. Plant Physiology 3 (2009): 1579-1592.
- HA Contreras-Cornejo., et al. “Trichoderma modulates stomatal aperture and leaf transpiration through an abscisic acid-dependent mechanism in Arabidopsis”. Journal of Plant Growth Regulation 2 (2015): 425-432.
- H Yuan., et al. “Microbial utilization of rice root exudates: 13C labeling and PLFA composition”. Biology and Fertility of Soils 5 (2016): 615-627.
- HA Contreras-Cornejo., et al. “Trichoderma improve growth of Arabidopsis seedlings under salt stress through enhanced root development, osmolite production, and Na+ elimination through root exudates”. Molecular Plant-Microbe Interactions 27.6 (2014): 503-514.
- AN Mukhopadhyay., et al. “Biological seed treatment for control of soil-borne plant pathogens”. Bulletin Phytosanitaire de la FAO (FAO); Boletin Fitosanitario de la FAO (FAO) (1992).
- K Sanjeev., et al. “Evaluation of Trichoderma species against Fusarium udum Butler causing wilt of pigeonpea”. Journal of Biological Control 3 (2009): 329-332.
- PK Mukherjee and AN Mukhopadhyay. “In situ mycoparasitism of Gliocladium virens on Rhizoctonia solani”. Indian Phytopathology 1 (1995): 101-102.
- BC Das and DK Hazarika. “Biological management of sheath blight of rice”. Indian Phytopathology 4 (2000): 433-435.
- S Sriram., et al. “Inactivation of phytotoxin produced by the rice sheath blight pathogen Rhizoctonia solani”. Canadian Journal of Microbiology 6 (2000): 520-524.
- D Singh and VK Maheshwari. “Biological seed treatment for the control of loose smut of wheat”. Indian Phytopathology 4 (2001): 457-460.
- JG Jat and HR Agalave. “Antagonistic properties of Trichoderma species against oilseed-borne fungi”. Scientific Research Reports 2 (2013): 171-174.
- DS Mishra., et al. “Comparative efficacy of normal seed treatment and seed biopriming with commercial formulations of Trichoderma spp”. in 53rd Annual meeting of Indian Phytopathological Society and National symposium on Ecofriendly approaches for Trichoderma Chennai, India (2001): 21-23.
- UI Baby and K Manibhushanrao. “Fungal antagonists and VA mycorrhizal fungi for biocontrol of Rhizoctonia solani, the rice sheath blight pathogen”. Recent Developments in Management of Plant Diseases. Today Tomorrow’s Printers Publ. New Delhi 1-9 (1996).
- S Kumar. “Integrated management of maydis leaf blight of maize”. Annals of Plant Protection Sciences 2 (2010): 536-538.
- V Kumar., et al. “Defense-related gene expression and enzyme activities in transgenic cotton plants expressing an endochitinase gene from Trichoderma virens in response to interaction with Rhizoctonia solani”. Planta2 (2009): 277-291.
- K Krishnamma and PN Reddy. “Efficacy of Trichoderma viride against Colletotrichum falcatum in sugarcane”. Indian Journal of Plant Protection 1/2 (2009): 111-115.
- RK Srivastava., et al. “Management of Macrophomina disease complex in jute (Corchorus olitorius) by Trichoderma viride”. Journal of Biological Control1 (2010): 77-79.
- A Mustafa., et al. “Mass multiplication of Trichoderma on organic substrate and their effect in management of seed borne fungi”. Pakistan Journal of Phytopathology 21.2 (2009): 108-114.
- NW Zaidi and US Singh. “14 Trichodermain Plant Health Management”. Trichoderma: Biology and Applications 230 (2013).
- P Vasudevan., et al. “Biological control of rice diseases”. Progress in Biological Control (2002): 11-32.
- JH Andrews. “Biological control in the phyllosphere”. Annual Review of Phytopathology 1 (1992): 603-635.
- AALI KHAN and AP Sinha. “Influence of different factors on the effectivity of fungal bioagents to manage rice sheath blight, in nursery”. Indian Phytopathology (2012).
- S Daljeet., et al. “Management of citrus scab caused by Elsinoe fawcettii”. Indian Phytopathology 4 (2000): 461-467.