Isolation and Molecular Identification of Fungi Associated with Stored Grains Sold
at Dawanau and Rimi Markets of Kano State, Nigeria
Sultan, Z.*, Nuhu, A.A,, Aminu, I.M,, Kamal, A.A. and Sumayya, S.S
Department of Biological Science, Yusuf Maitama Sule University, Kano, Nigeria
*Corresponding Author: Sultan Z, Department of Biological Science, Yusuf Maitama Sule University, Kano, Nigeria.
March 14, 2022; Published: May 27, 2022
Plant pathogenic fungi belong to the Kingdom Fungi comprising of over 100,000 registered species grouped into about 4,300 genera. Many of these fungi infect a variety of cereals particularly stored condition. A study was conducted in the Dawanau and Rimi Markets of Kano state of Nigeria to quantify the number, types and frequency of occurrence of Plant pathogenic fungi associated with stored grains in these markets. The total fungal load and pathogenicity test was also conducted besides subjecting the fungal load for exact identification using the Molecular Biology Technique. A total of seven (7) fungal isolates were isolated from the collected grains. It was found that Genus Aspergillus was the most frequent isolate (44.8%) from the two markets combined while Alternaria spp. was the least reported fungi (6.9%) from the grains collected from these two markets. Some species of Aspergillus are known to produce Aflatoxins. A combination of many different isolates was present on the grains. The molecular identification method using Polymerase Chain Reaction (PCR) helped in the identification of fungi at the species level with precision and in the least possible time. The study concludes that there is need for having a National Level, Germplasm collection centre and detailed database of all the naturally occurring post-harvest Plant pathogenic fungi in Nigeria for easy intervention and timely overcoming of situations in case of any emergency.
Keywords: Fungi Identification; PCR; Aflatoxins; Plant Pathogenic Fungi; Stored Grains
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