Induced Mutants in Locally Adapted Landraces of French Bean (Phaseolus vulgaris L.), their Mutagenic Sensitivity and Mutability for Crop Improvement

Sanjeev Kumar¹*, Mohar Singh², Nikhil Malhotra², Archna Joshi Saha³, Sanjay Jambhulkar³, JP Sharma⁴ and Manmohan Singh¹

¹Pulses Research Sub-Station Samba, SKUAST-Jammu, India

²ICAR-National Bureau of Plant Genetic Resources Regional Station Shimla, India

³Nuclear Agriculture and Biotechnology Division, BARC Mumbai, Maharashtra, India

⁴Directorate of Research, SKUAST-Jammu, India

*Corresponding Author: Sanjeev Kumar, Pulses Research Sub-Station Samba, SKUAST-Jammu, India.

Received: August 21, 2021; Published: November 19, 2021

Abstract

Mutagenesis is an important breeding strategy to enhance mutation frequency which enables studies of functional genomics and development of new genotypes. An attempt was made to induce mutagenesis into named landraces of french bean viz. Bhaderwah Local (BL) and Poonch Local (PL) with gamma rays and ethyl methane sulphonate (EMS) to determine their sensitivity and mutability. Landrace PL was more sensitive than BL to both the mutagens. The LD₅₀ of germination percentage for BL and PL was determined as 248.058 Gy and 234.167 Gy, respectively. The maximum number of chlorophyll mutants was found in BL than PL to both mutagens. Gamma rays doses were found to be more effective in both the landraces for the generation of chlorophyll mutants. Mutants in M₂ generation of BL screened under 200 Gy and 250 Gy showed high resistance against anthracnose and moderate resistance of EMS induced mutants under 150 Gy and 225 Gy doses. These genetic stocks could be useful for allele mining and creation of variability for widening the genetic base of traditional landraces of french bean.

 

Keywords: Phaseolus vulgaris; LD₅₀; Chlorophyll Mutations; Mutagen Sensitivity; Radio Sensitivity; Crop Improvement

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Citation

Citation: Sanjeev Kumar., et al. “Induced Mutants in Locally Adapted Landraces of French Bean (Phaseolus vulgaris L.), their Mutagenic Sensitivity and Mutability for Crop Improvement". Acta Scientific Agriculture 5.12 (2021): 10-16.

Copyright

Copyright: © 2021 Sanjeev Kumar., et al.This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.