Acta Scientific Agriculture (ASAG)(ISSN: 2581-365X)

Research Article Volume 5 Issue 12

Induced Mutants in Locally Adapted Landraces of French Bean (Phaseolus vulgaris L.), their Mutagenic Sensitivity and Mutability for Crop Improvement

Sanjeev Kumar1*, Mohar Singh2, Nikhil Malhotra2, Archna Joshi Saha3, Sanjay Jambhulkar3, JP Sharma4 and Manmohan Singh1

1Pulses Research Sub-Station Samba, SKUAST-Jammu, India

2ICAR-National Bureau of Plant Genetic Resources Regional Station Shimla, India

3Nuclear Agriculture and Biotechnology Division, BARC Mumbai, Maharashtra, India

4Directorate of Research, SKUAST-Jammu, India

*Corresponding Author: Sanjeev Kumar, Pulses Research Sub-Station Samba, SKUAST-Jammu, India.

Received: August 21, 2021; Published: November 19, 2021


Mutagenesis is an important breeding strategy to enhance mutation frequency which enables studies of functional genomics and development of new genotypes. An attempt was made to induce mutagenesis into named landraces of french bean viz. Bhaderwah Local (BL) and Poonch Local (PL) with gamma rays and ethyl methane sulphonate (EMS) to determine their sensitivity and mutability. Landrace PL was more sensitive than BL to both the mutagens. The LD50 of germination percentage for BL and PL was determined as 248.058 Gy and 234.167 Gy, respectively. The maximum number of chlorophyll mutants was found in BL than PL to both mutagens. Gamma rays doses were found to be more effective in both the landraces for the generation of chlorophyll mutants. Mutants in M2 generation of BL screened under 200 Gy and 250 Gy showed high resistance against anthracnose and moderate resistance of EMS induced mutants under 150 Gy and 225 Gy doses. These genetic stocks could be useful for allele mining and creation of variability for widening the genetic base of traditional landraces of french bean.


Keywords: Phaseolus vulgaris; LD50; Chlorophyll Mutations; Mutagen Sensitivity; Radio Sensitivity; Crop Improvement


  1. A1-Rubeai MAF. “Radio-sensitivity of dormant Phaseolus seeds”. Journal Environmental and Experimental Botany 21 (1980): 71-74.
  2. Badere RS and Choudhry AD. “Effectivity and efficiency of gamma rays, sodium azide and ethyl methane sulphonate in Linseed”. Bioinfolet3 (2007): 181-187.
  3. Blixt S and Gottschalk W. “Mutation breeding in Leguminosae”. 33 (1975): 33-85.
  4. Brendel M., et al. “Common steps in the repair of alkylation and repair damage in yeast”. Molecular Genetics and Genomics 106 (1970): 289-295.
  5. Dhanavel D., et al. “Effectiveness and efficiency of chemical mutagens in Cowpea (Vigna unguiculata (L.) Walp.)”. The African Journal of Biotechnology 7 (2008): 4116-4117.
  6. Ellyfa K., et al. “Gamma Radio sensitivity Study on Snap Bean (Phaseolus vulgaris L.)”. International Journal of Agricultural Research9 (2007): 844-848.
  7. Gaul H. “Mutations in plant breeding for forage and grain”. Rad. on Bot 4.3 (1964): 151-232.
  8. Goyal S., et al. “Frequency and spectrum of chlorophyll mutations induced by single and combination treatments of gamma rays and EMS in Urdbean”. Asian Journal of Bio Science 12 (2019): 156-163.
  9. Jain SM. “Mutagenesis in crop improvement under the climate change”. Romanian Biotechnological Letters2 (2010): 88-106.
  10. Khar kwal MC. “A brief history of plant mutagenesis”. In: Q. Y. Shu, B. P. Forster and H. Nakagawa (Editions.). Plant Mutation Breeding and Biotechnology (2012): 21.
  11. Khar kwal MC. “Induced mutations in chickpea, frequency and spectrum of viable mutations”. The Indian Journal of Genetics and Plant Breeding4 (1999): 451-464.
  12. Kiong A., et al. “Physiological responses of Orthosiphon stamineus plantlets to gamma irradiation”. American-Eurasian Journal of Sustainable Agriculture 2 (2008): 135-139.
  13. Kumar S., et al. “Induced chlorophyll mutations and mutagenic effectiveness and efficiency of ethyl methane sulphonate in horsegram (Macrotyloma uniflorum Roxb)”. Green Farming5 (2012): 151-54.
  14. Kumar S. “Mutagenic effectiveness and efficiency in macrosperma lentil under mid hills of northwestern Himalayas”. Journal of Crop Improvement2 (2013): 182-185.
  15. Lekharam J. “Induced genetic variability through physical and chemical mutagens in M3 and M4 populations of French bean (Phaseolus vulgaris L.)”. The Indian Journal of Plant Genetic Resources1 (2010): 44-50.
  16. Mishra SD., et al. “Radiation induced delay in organogenesis of Kalanchoe daigremntiana leaves”. Journal Environmental and Experimental Botany 20 (1980): 309-323.
  17. Oladosu Y., et al. “Principle and application of plant mutagenesis in crop improvement: a review”. Biotechnology and Biotechnological Equipments1 (2016): 1-16.
  18. Padmavathi T., et al. “Induced variability for different biological parameters in soybean”. Journal of Cytology and Genetics 27 (1992): 175-177.
  19. Pavadai P., et al. “Effect of gamma rays, EMS, DES and COH on protein and oil content in soybean”. The Journal of Eco Biotechnology4 (2010): 47-50.
  20. Puchooa D. “In-vitro mutation breeding of Anthurium by gamma radiation”. International Journal of Agriculture and Biology1 (2005): 11-20.
  21. Sagel Z., et al. “Nohutta Mutasyon Islahı”. In Ulusal nükleer bilimler ve teknolojileri kongresi (VIII Nat. Cong. of Nucl. Sci. and Techn.) (2009): 313-320.
  22. Salve KM and More KD. “Effect of gamma radiation on seed germination, seedling height, seedling injury in Coriandrum sativum”. International Journal of Life Science 2 (3): 223-25.
  23. Sangsiri C., et al. “Gamma radiation induced mutations in mungbean”. Science Asia (2005): 251-255.
  24. Sharma SK and Sharma B. “Mutagen sensitivity and mutability in lentil”. Theoretical and Applied Genetics 71 (1986): 820-825.
  25. Sikder S., et al. “Induction of mutation in tomato by gamma irradiation and EMS”. The Indian Journal of Genetics and Plant Breeding 4 (2013): 392-399.
  26. Suresh D., et al. “Determination of lethal dose for gamma rays induced mutagenesis in butter bean (Phaseolus lunatus L.) variety KKL-1”. International Journal of Current Microbiology and Applied Sciences 3 (2017): 712-717.
  27. Thilagavathi C and Mullainathan L. “Isolation of macro mutants and mutagenic effectiveness, efficiency in black gram”. Global International Journal of Molecular Sciences 4.2 (2009): 76-79.
  28. Ulukapi K and Ozmen SF. “Study of the effect of irradiation (60Co) on M1 plants of common bean cultivars and determined of proper doses for mutation breeding”. Journal of Radiation Research and Applied Sciences 11 (2018): 157-161.
  29. Yankulov MT., et al. “Algunos aspectos sobre la sensibilidady mutablidad de dosvariedadesdefrijol por influencia de raoyos gamma Co60 y etil-metan-sulfonator (EMS)”. Ciencias de la Agricultura 7 (1980): 59-64.


Citation: Sanjeev Kumar., et al. “Induced Mutants in Locally Adapted Landraces of French Bean (Phaseolus vulgaris L.), their Mutagenic Sensitivity and Mutability for Crop Improvement". Acta Scientific Agriculture 5.12 (2021): 10-16.


Copyright: © 2021 Sanjeev Kumar., et al.This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


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