Induced Mutants in Locally Adapted Landraces of French Bean (Phaseolus vulgaris L.),
their Mutagenic Sensitivity and Mutability for Crop Improvement
Sanjeev Kumar1*, Mohar Singh2, Nikhil Malhotra2, Archna Joshi Saha3, Sanjay Jambhulkar3, JP Sharma4 and Manmohan Singh1
1Pulses Research Sub-Station Samba, SKUAST-Jammu, India
2ICAR-National Bureau of Plant Genetic Resources Regional Station Shimla, India
3Nuclear Agriculture and Biotechnology Division, BARC Mumbai, Maharashtra, India
4Directorate of Research, SKUAST-Jammu, India
*Corresponding Author: Sanjeev Kumar, Pulses Research Sub-Station Samba, SKUAST-Jammu, India.
August 21, 2021; Published: November 19, 2021
Mutagenesis is an important breeding strategy to enhance mutation frequency which enables studies of functional genomics and development of new genotypes. An attempt was made to induce mutagenesis into named landraces of french bean viz. Bhaderwah Local (BL) and Poonch Local (PL) with gamma rays and ethyl methane sulphonate (EMS) to determine their sensitivity and mutability. Landrace PL was more sensitive than BL to both the mutagens. The LD50 of germination percentage for BL and PL was determined as 248.058 Gy and 234.167 Gy, respectively. The maximum number of chlorophyll mutants was found in BL than PL to both mutagens. Gamma rays doses were found to be more effective in both the landraces for the generation of chlorophyll mutants. Mutants in M2 generation of BL screened under 200 Gy and 250 Gy showed high resistance against anthracnose and moderate resistance of EMS induced mutants under 150 Gy and 225 Gy doses. These genetic stocks could be useful for allele mining and creation of variability for widening the genetic base of traditional landraces of french bean.
Keywords: Phaseolus vulgaris; LD50; Chlorophyll Mutations; Mutagen Sensitivity; Radio Sensitivity; Crop Improvement
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