Acta Scientific Agriculture (ASAG)(ISSN: 2581-365X)

Research Article Volume 5 Issue 11

Callus Induction from Leaf and Stem Explants of Selected Turkish Genotypes of Common Bean

Asli Küçükrecep and Dilek Tekdal*

Department of Biotechnology, Faculty of Science and Letters, Mersin University, Mersin, Turkey

*Corresponding Author: Dilek Tekdal, Department of Biotechnology, Faculty of Science and Letters, Mersin University, Mersin, Turkey.

Received: September 21, 2021; Published: October 05, 2021

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Abstract

Common bean (Phaseolus vulgaris L.), a species belonging to the family Fabaceae, is one of the most critical legumes consumed and grown globally. Common bean ranks first among grain legumes in terms of cultivation area and production in the world. However, the bean is recalcitrant, making obtaining this plant via in vitro culture techniques a challenge. Due to changing climatic factors and drought conditions that may happen in the future, it is essential to produce plants that make up human food in vitro conditions. With this goal, the effect of different concentrations of 2,4-D and Kinetin and their possible combinations on callus induction in leaf and stem explants of three-bean genotypes (Bitlis117, Göynük, and Önceler) were evaluated. It aimed to determine the best medium that promotes callus formation in different bean genotypes and tissue types. Callogenesis was successfully promoted from leaf and stem explants of all three genotypes tested. The highest callus ratio was obtained from stem explants of Bitlis117 cultured on the MS medium, including 2,4-D (0.5mg L -1) and Kinetin (2.0 mg L -1).

Keywords: In vitro Culture; Leaf; Mersin; Phaseolus vulgaris L.; Stem

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Citation

Citation: Asli Küçükrecep and Dilek Tekdal. “Callus Induction from Leaf and Stem Explants of Selected Turkish Genotypes of Common Bean". Acta Scientific Agriculture 5.11 (2021): 02-05.




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