Trehalose Production at Different Mediums in Bacillus cereus and Ralstonia eutropha

Cennet Canan Karaderi¹* and Hüseyin Kahraman²

¹Department of Biology, Institute of Science and Technology, İnönü University, Turkey
²Department of Biology, Faculty of Arts and Sciences, İnönü University, Turkey

*Corresponding Author: Cennet Canan Karaderi, Department of Biology, Institute of Science and Technology, İnönü University, Turkey.

Received: August 10, 2021; Published: September 07, 2021

Citation: Cennet Canan Karaderi and Hüseyin Kahraman. “Trehalose Production at Different Mediums in Bacillus cereus and Ralstonia eutropha". Acta Scientific Agriculture 5.10 (2021): 02-04.

Abstract

  Trehalose; It is a non-reducing disaccharide consisting of 2 glucose molecules, which is abundant in nature. This disaccharide is a sugar that serves both as an important storage carbohydrate for living things and as a protector against various environmental stresses (drought, freezing, excessive salt environment, etc.). It can produce many living species, from bacteria to insects and invertebrates. The aim of this study was to compare the production of trehalose produced by Bacillus cereus Gr (+) and Ralstonia eutropha Gr (-) in different carbon sources. The highest Trehalose production in NB (Nutrient Broth) medium was found 172,235(U/ml) in Bacillus cereus at 150 rpm. The highest trehalose production in environments containing different carbon sources (1% Glucose, 1% Fructose, 1% Dextrose, 1% Xylose, 1% Maltose, 1% Ramnose, 1% Ribose); It was found 20,794 (U/ml) in B. cereus in PBS + maltose broth and 17,485 (U/ml) in PBS + ribose broth in R. eutropha. The lowest trehalose production was found to be 7,917 (U/ml) in B. cereus in PBS medium. In this study, it was observed that R. eutropha, a good PHB (Polyhydroxy butyrate) producer, and B. cereus, an endospore-forming soil bacterium, were able to produce trehalose in rich and poor, aeration conditions (0, 150 rpm). This study shows that inexpensive and suitable media can be used to produce trehalose, an important metabolite. Many Gr (t) and Gr (-) bacterial groups seem to be an important microbial source preferred for trehalose production.

Key words: Trehalose; Bacillus cereus; Ralstonia eutropha

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Copyright: © Cennet Canan Karaderi and Hüseyin Kahraman. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.