Acta Scientific Agriculture (ISSN: 2581-365X)

Research ArticleVolume 5 Issue 10

Trehalose Production at Different Mediums in Bacillus cereus and Ralstonia eutropha

Cennet Canan Karaderi1* and Hüseyin Kahraman2

1Department of Biology, Institute of Science and Technology, İnönü University, Turkey
2Department of Biology, Faculty of Arts and Sciences, İnönü University, Turkey

*Corresponding Author: Cennet Canan Karaderi, Department of Biology, Institute of Science and Technology, İnönü University, Turkey.

Received: August 10, 2021; Published: September 07, 2021

Citation: Cennet Canan Karaderi and Hüseyin Kahraman. “Trehalose Production at Different Mediums in Bacillus cereus and Ralstonia eutropha". Acta Scientific Agriculture 5.10 (2021): 02-04.

Abstract

  Trehalose; It is a non-reducing disaccharide consisting of 2 glucose molecules, which is abundant in nature. This disaccharide is a sugar that serves both as an important storage carbohydrate for living things and as a protector against various environmental stresses (drought, freezing, excessive salt environment, etc.). It can produce many living species, from bacteria to insects and invertebrates. The aim of this study was to compare the production of trehalose produced by Bacillus cereus Gr (+) and Ralstonia eutropha Gr (-) in different carbon sources. The highest Trehalose production in NB (Nutrient Broth) medium was found 172,235(U/ml) in Bacillus cereus at 150 rpm. The highest trehalose production in environments containing different carbon sources (1% Glucose, 1% Fructose, 1% Dextrose, 1% Xylose, 1% Maltose, 1% Ramnose, 1% Ribose); It was found 20,794 (U/ml) in B. cereus in PBS + maltose broth and 17,485 (U/ml) in PBS + ribose broth in R. eutropha. The lowest trehalose production was found to be 7,917 (U/ml) in B. cereus in PBS medium. In this study, it was observed that R. eutropha, a good PHB (Polyhydroxy butyrate) producer, and B. cereus, an endospore-forming soil bacterium, were able to produce trehalose in rich and poor, aeration conditions (0, 150 rpm). This study shows that inexpensive and suitable media can be used to produce trehalose, an important metabolite. Many Gr (t) and Gr (-) bacterial groups seem to be an important microbial source preferred for trehalose production.

Key words: Trehalose; Bacillus cereus; Ralstonia eutropha

Bibliography

  1. Suthisawat S., et al. “Functional and structural analysis of trehalose-6-phosphate phosphatase from Burkholderia pseudomallei: Insights into the catalytic mechanism”. Biochemical and Biophysical Research Communications 523 (2020): 979-984.
  2. Song L., et al. “Progress in Enzymatic production of trehalose”. Agricultural Biotechnology 10 (2021): 87-90.
  3. Marathe SJ., et al. “Enzymatic synthesis of fatty acid esters of trehalose: Process optimization, characterization of the esters and evaluation of their bioactivities”. Bioorganic Chemistry 94 (2020): 1-13.
  4. Qiao Y., et al. “Engineering cyanobacteria as cell factories for direct trehalose production from CO2”. Metabolic Engineering 62 (2020): 161-171.
  5. Chaitanya N S N., et al. “Molecular mechanisms of action of Trehalose in cancer: A comprehensive review”. Life Sciences 269 (2021): 1-6.
  6. Öcal Demir S., et al.Bacillus cereus’ un Etken Olduğu Kateter İlişkili Kan Akımı Enfeksiyonu”. Çocuk Dergisi 16 (2016): 39-42.
  7. Tuipulotu DE., et al. “Bacillus cereus: Epidemiology, Virulence Factors, and Host–Pathogen Interactions”. Trends in Microbiology 29 (2021): 458-471.
  8. Özdemir Atay İ. “Bazı pestisitlerin Ralstonia eutropha ile gideriminin incelenmesi”. Yüksek Lisans Tezi, Fırat Üniversitesi, Elazığ (2007).
  9. Lee HM., et al. “Microbial production of ethanol from acetate by engineered Ralstonia eutropha”. Biotechnology and Bioprocess Engineering 21 (2016): 402-407.
  10. Xiong B., et al. “Genome editing of Ralstonia eutropha using an electroporation-based CRISPR-Cas9 technique”. Biotechnology for Biofuels 11 (2018): 1-9.
  11. Karaderi CC. “Pseudomonas aeruginosa, Escherichia coli ve Enterococcus faecalis’ de farklı ortam koşullarında proteaz, prolin ve biofilm üretimi ile kayma hareketlerinin incelenmesi”. Yüksek Lisans Tezi, İnönü Üniversitesi, Malatya (2016).
  12. Mei YZ., et al. “Cold stress promoting a psychrotolerant bacterium Pseudomonas fragi P121 producing trehaloase”. World Journal of Microbiology and Biotechnology 32 (2016): 1-9.

Copyright: © Cennet Canan Karaderi and Hüseyin Kahraman. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.



News and Events


  • Certification for Review
    Acta Scientific certifies the Editors/reviewers for their review done towards the assigned articles of the respective journals.
  • Submission Timeline for Upcoming Issue
    The last date for submission of articles for regular Issues is December 25, 2024.
  • Publication Certificate
    Authors will be issued a "Publication Certificate" as a mark of appreciation for publishing their work.
  • Best Article of the Issue
    The Editors will elect one Best Article after each issue release. The authors of this article will be provided with a certificate of "Best Article of the Issue"

Contact US





//