Genome Editing Technology in Plant Breeding: A Review

Rashmi Regmi¹*, Bishnu Bhusal¹, Pritika Neupane¹, Kushal Bhattarai¹, Binju Maharjan¹, Suprava Acharya¹, Bigyan KC¹, Rishav Pandit¹, Ram Prashad Mainali² and Mukti Ram Poudel³

¹Department of Plant Breeding, Tribhuwan University, Kathmandu, Bagmati Province, Nepalal
²Technical Officer, Nepal Agricultural Research Council, Khumaltar, Bagmati Province, Nepalal
³Assistant Professor, Department of Plant Breeding, Tribhuwan University, Kathmandu, Bagmati Province, Nepal

*Corresponding Author: Rashmi Regmi, Department of Plant Breeding, Tribhuwan University, Kathmandu, Bagmati Province, Nepal.

Received: March 12, 2021; Published: July 28, 2021

Citation: Rashmi Regmi., et al. “Genome Editing Technology in Plant Breeding: A Review". Acta Scientific Agriculture 5.8 (2021): 41-47.

Abstract

Global population growth is demanding for more food production in the future. The selection of the plant for cropping has led to the loss of valuable genotypes. Genome-editing technology is the recent advances in plant breeding which allows the insertion, deletion or substitution of the specific loci in the target host cell. Among the homologous recombination (HR) technology, transcription activation-like effector nuclease (TALEN), zinc finger nucleases (ZFN) and clustered regularly interspaced short palindromic repeats (CRISPR); the most popular these days is CRISPR/Cas. Here, we review the methods of genome-editing, their applications, potentials, and the regulatory issues related to genetically modified organisms.

Keywords: Genome-editing; Homologous Recombination; CRISPR/Cas; Indels; Crop Improvements

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