Physiological Interactions of Phaseolus vulgaris L. with Telfairia Mosaic Virus

AAJ Mofunanya*, EA Effa, BA Ngele, FA Akomaye and AO Damian

Department of Botany, Faculty of Biological Sciences, University of Calabar, Cross River State, Nigeria

*Corresponding Author: AAJ Mofunanya, Department of Botany, Faculty of Biological Sciences, University of Calabar, Cross River State, Nigeria.

Received: May 12, 2021; Published: May 26, 2021

Abstract

  The study was conducted to investigate the physiological outcome of interactions of Phaseolus vulgaris L. with Telfairia mosaic virus (TeMV). Seeds of P. vulgaris used in this study were obtained from a farm where the plants were monitored on the field for virus symptoms expression. The seeds were sown, on germination inoculated with the virus at the two leaf stage and allowed to stay for symptom expression. Relative water content, leaf nitrogen content, photosynthetic pigments and enzymes activities were determined at different stages of growth to quantify host virus interactions physiologically. Due to virus infection, some physiological parameters like photosynthetic pigments (chlorophyll a, b and carotenoids), relative water content (RWC), leaf nitrogen content were drastically reduced while all enzymes activity increased significantly. Interaction of P. vulgaris with TeMV led to decreased in RWC and leaf nitrogen content. Percentage decreased in RWC of 40.1% at 12 WAI and 57.6% for leaf nitrogen at 2 WAI was found. Telfairia mosaic virus interaction caused reduction in leaf chlorophyll a content of 53.6%, chlorophyll b (32.9%), chlorophyll a + b (46.6%), ratio of chlorophyll a/b (30.8%) and carotenoids (50.0%). The virus interactions engendered increase in the activities of peroxidase (POD), polyphenol oxidase (PPO), glucose-6-phosphate dehydrogenase (G6PdH), 6-phosphogluconate dehydrogenase (6PGdH) enzymes of 57.0%, 64.9%, 90.4%, 99.1% respectively at 8 WAI. Interactions brought about changes in host physiology with variations in quantity of physiological components affecting whole plant functioning.

Keywords: Enzymes Activities; Interaction; Photosynthetic Pigments; Phaseolus vulgaris

References

1. Munoz-Huerta R F., et al. “A review of methods for sensing the nitrogen status in plants: advantages, disadvantages and recent advances”. Sensor8 (2013): 10823-10843.
2. Hull R. “Matthew’s plant virology”. London, Academic Press Incorporated (2002).
3. Efeoglu B., et al. “Physiological responses of three maize cultivars to drought stress and recovery”. South African Journal of Botany 75 (2009): 34-42.
4. Lichthenthaler H K. “Chlorophylls and carotenoids: pigments of photosynthetic biomembranes”. Methods in Enzymology 148 (1987): 350-382.
5. Kahn V. “Multiple effects of hydrogen peroxide on the activity of avocado polyphenol oxidase”. Phytochemistry 22 (1983): 2155-2159.
6. Gosling PG and Ross JD. “Characterization of glucose-6-phosphate dehydrogenase and 6-phosphogluconic acid dehydrogenase from hazel cotyledons”. Phytochemistry 18 (1979): 1441-1445.
7. Pazarlar S., et al. “The effects of Tobacco mosaic virus infection on growth and physiological parameters in some pepper varieties (Capsicum annuum )”. Notulae Botanicae Horti Agrobotanici Cluj-Napoca 41.2 (2013): 427-433.
8. Rys M., et al. “Comparison of a compatible and an incompatible pepper-tobamovirus interaction by biochemical and non-invasive techniques: chlorophyll a fluorescence, isothermal calorimetry and FT-Raman spectroscopy”. Plant Physiology and Biochemistry 83 (2014): 267-278.
9. Nursu aidah H. “Growth and photosynthetic responses of long bean (Vigna unguiculata) and mung bean (Vigna radiata) response to fertilization”. Journal of Animal Physiology 24 (2014): 573-578.
10. Mofunanya A A J., et al. “Effects of Telfairia mosaic virus (TeMV) on the chlorophyll content and photosynthetic capabilities of two ecotypes of Telfairia occidentalis F. (Fluted pumpkin)”. International Journal of Plant Pathology 5.2 (2014): 54-64.
11. Jabeen A., et al. “Variation in chlorophyll and carotenoid contents in Tungro infected plants”. Journal of Research Development1 (2017): 1000153.
12. Papaiah S., et al. “Symptomlogical and biochemical studies on sunflower necrosis disease in sunflower plants in Rayalaseema region of Andhra Pradesh, India”. Annals of Biological Research1 (2012): 170-178.
13. Hosikian A., et al. “Chlorophyll extraction from microalgae: A review on the process engineering aspects”. International Journal of Chemical Engineering (2010): 11.
14. Qaisar U., et al. “Commercial Applications of Plant Pigments”. International Journal of Biotech Trends and Technology3 (2019).
15. Souza P F N., et al. “Photosynthetic and biochemical mechanisms of an EMS-mutagenized cowpea associated with its resistance to cowpea severe mosaic virus”. Plant Cell Report 36 (2017): 234.
16. Rahman Md S., et al. “Effects of Cucumber mosaic virus on cellular components, host physiology and yield”. Plant Tissue Culture and Biotechnology2 (2019): 219-230.
17. Yoneyama K., et al. “Conversion of carlactone to carlactonic acid is a conserved function of MAX homologs in strigolactone biosynthesis”. New Phytologist4 (2018): 1522-1533.
18. Finkelstein R. “Ascisic acid synthesis and response”. The Arabidopsis Book/American Society of Plant Biologists 11 (2013): e0166.
19. Riedle-Bauer M. “Role of reactive oxygen species and antioxidant enzymes in systemic virus infections of plants”. Journal of Phytopathology 148 (2000): 297-302.
20. Taranto F., et al. “Polyphenol oxidases in crops: biochemical, physiological and genetic aspects”. International Journal of Molecular Science 18 (2017): 377.
21. Araji S., et al. “Novel roles for the phlyphenol oxidase enzyme in secondary metabolism and the regulation of cell death in walnut”. Plant Physiology (2014): 113.
22. Mofunanya A A J., et al. “ Changes in some enzymes activities of Amaranthus viridis inoculated with Telfairia mosaic virus (TeMV)”. Journal of Applied Life Sciences International 15.3 (2017): 1-11.
23. Janeczko A., et al. “Comparative studies of compatible and incompatible pepper–Tobamovirus interactions and the evaluation of effects of 24-epibrassinolide”. Photosynthetica3 (2018): 763-775.
24. Hull R. “Effects on plant metabolism”. In: Comparative plant virology, 2^nd Academic Press, Norwich (2009): 181-185.

Citation

Citation: AAJ Mofunanya., et al. “Physiological Interactions of Phaseolus vulgaris L. with Telfairia Mosaic Virus". Acta Scientific Agriculture 5.6 (2021): 102-108.

Copyright

Copyright: © 2021 AAJ Mofunanya., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.