Morpho-biometric and Cytogenetic analysis of Clone-selected Mulberry Cultivars (Morus Spp.)
Ravi Kumara R1, HL Ramesh2 and HB Manjunatha1*
1Department of Studies in Sericulture Science, University of Mysore, Mysuru, Karnataka, India
2Department of Sericulture, Visveswarapura College of Science, Bengaluru, Karnataka, India
*Corresponding Author: HB Manjunatha, Department of Studies in Sericulture Science, University of Mysore, Mysuru, Karnataka, India.
May 03, 2021; Published: May 26, 2021
Genetic variation is uncommon in mulberry (Morus spp.) cultivars due to its asexual propagation and shows the considerable phenotypic plasticity to adopt different agro-climatic conditions. These open an ample scope for the selection of superior clones for better leaf yield and quality. To uncover this cryptic feature, we have selected clonally evolved mulberry cultivars TG-1, Anantha, and Vishala and compared them with their putative mother plants M-5, RFS-135, and S-1635 respectively. Interestingly, significant variations are obvious in all clonally-selected mulberry cultivars with their respective putative mother plants. Comparatively, the plant height, the number of branches, leaf yield, and survival percentage were higher in TG-1, Anantha, and Vishala over M-5, RFS-135, and S-1635 cultivars respectively. Furthermore, altered anatomical structures - thick cuticle, fewer and smaller stomata in TG-1, Anantha, and Vishala leaves are positively correlated with moisture conservation and drought adaptation. Besides, clonally-selected mulberry cultivars had significantly higher leaf moisture, soluble protein, and sugar contents. The cytological evidence shows that RFS-135 and Anantha have 2n=2x=28 (Diploids), M-5 and TG-1 also exhibit 2n=2x=28; but Vishala and S-1635 possess 2n=3x=42 (Triploids). We hypothesize that discrete morphological, anatomical, and biochemical variations in clonally-selected mulberry cultivars compared to their putative mother plants are due to no gain or loss in chromosomal complements, rather to repetitive DNA sequence or related epigenetic modifications.
Keywords: Mulberry; Chromosome; Clonal Selection; TG-1; Anantha; Vishala
- Laltanmawii and S Roychowdhuri. “Effects of chromosomal variations on morphology and leaf anatomical behaviors in mulberry (Morus )". Journal of Crop and Weed 6 (2010): 35-39.
- Tikader AK., et al. "Studies on sexual variation in mulberry (Morus)". Euphytica 84 (1995): 115-120.
- Wessler SR. “Plant retrotransposons: turned on by stress". Current Biology 6 (1996): 959-961.
- Grandbastien MA. “Activation of plant transposons under stress condition". Trends in Plant Science 3 (1998): 181-187.
- Vijayan K., et al. "Diversification of mulberry (Morus indica S-36), a vegetatively propagated tree species". Caspian Journal of Environmental Sciences 7 (2009): 23-30.
- Burton GW. "Quantitative inheritance in grasses". Proc. Int. Grassland Congr. 1 (1952): 277-283.
- Mullin R and FI Lauer. “Breeding behavior of F1 and inbred potato clones". Proceedings of the American Society for Science 89 (1966): 449-455.
- Mariotti JA. “The effect of environments on the effectiveness of clonal selection in sugarcane”. ISWT 15 (1974): 89-95.
- Singh RN., et al. “Improvement of ‘Banarasi’, ‘Langra’ through clonal selection". Progressive Horticulture 17 (1985): 273-277.
- Ortiz R. “Musa Genetics. In Gowen, ed. Bananas and Plantains". UK Chapman and Hall. (1995): 10-84.
- Tikader A and CK Kamble. "Mulbrry breeding in India - A Critical Review”. Sericologia 47 (2007): 359-366.
- Vijayan K., et al. "Studies on the leaf moisture on mulberry germplasm varieties". Indian Journal of Sericulture 36 (1997): 155-157.
- Ghosh MK., et al. “Yield stability in mulberry over different regions of Eastern and North-Eastern India". Journal of Crop and Weed 9 (2013): 103-105.
- Reddy MP., et al. “Anantha - a promising mulberry variety suitable for semi-arid conditions". Advances in Plant Sciences 23 (2010): 551-553.
- Saratchandra B., et al. "New mulberry varieties authorized for cultivation - A Report”. (2016).
- Thangavelu K., et al. “Catalogue on mulberry (Morus ) germplasm”. 2 (2000): 1-225.
- Mallikarjunappa RS., et al. "Leaf anatomical variations in Morus laevigata (Moraceae) complex". IJ Series 46 (2007): 92-95.
- Lowry OH., et al. “Protein measurement with folin phenol reagent”. Journal of Biological Chemistry 193 (1951): 265-275.
- Dubios SM., et al. “A colorimetric method of estimation of sugars and related substances". Analytical Chemistry 28 (1956): 350-356.
- Thangavelu K., et al. "Catalogue on mulberry (Morus) germplasm". 1 (1997): 1-225.
- Sharma AK and A Sharma. "Chromosome Techniqus. theory and practice". Butterworths, London, Third edition (1980).
- Ghader Mirzaghaderi and Karim Marzangi. “IdeoKar: an ideogram constructing and karyotype analyzing software”. Caryologia 68 (2015): 31-35.
- Yamanouchi H., et al. “Flow cytometric analysis of various organs and cytochimeras of mulberry (Morus )". Journal Insect Biotechnology and Sericology 77 (2008): 95-108.
- Zohary D. "Modes of evolution under domestication". In: Grant W, ed. Plant Biosystematics. Toronto, Canada: Academic Press. (1984): 579-596.
- Pelsy F. "Molecular and cellular mechanisms of diversity within grapevine varieties". Heredity 104 (2010): 331-340.
- Dandin SB., et al. "Mutant forms in mulberry (Morusspp)". Sericologia 36 (1996): 353-358.
- Jablonka E and G Raz. "Transgenerational epigenetic inheritance: prevalence, mechanisms, and implications for the study of heredity and evolution". The Quarterly Review of Biology 84 (2009): 131-176.
- Shu-Fen Li., et al. "Chromosome evolotion in connection with repetitive sequence and epigenetics in plants - A Review”. Genes 8 (2017).
- Uozu S., et al. "Repetitive sequences: cause for variation in genome size and chromosome morphology in the genus Oryza". Plant Molecular Biology 35 (1997): 791-799.
- Sakamoto K., et al. "Characterization: genome sizes and morphology of sex chromosomes in Hemp (Cannabis sativa)". Cytologia 63 (1998): 459-464.
- Soltis DE., et al. "Polyploidy: Recurrent formation and genome evolution". Trends in Ecology and Evolution 14 (1999): 348-352.
- Nicholas Panchy., et al. "Evolution of gene duplication in plants". Plant Physiology 171 (2016) :2294-2316.
- Nyine M and M Pillay. “The effect of banana breeding on the diversity of East African highland banana". Acta Horticulture (2010).
- Wang RL., et al. "Plant phylogeny and growth form as drivers of the altitudinal variation in woody leaf vein traits". Frontiers in Plant Science10 (2020): 17-35.
- Simcha Lev-Yadun., et al. "Colour patterns in vegetative parts of plants deserve more research attention". Trends in Plant Science7 (2002).
- Marcel E., et al. “Severely reduced sexual reproduction in northern populations of a clonal plant, Decodon verticillatus (Lythraceae)". Journals of Ecology 10 (2001): 1365-2745.
- Krstic LN., et al. "The variability of leaf anatomical characteristics of Solanum nigrum (Solanaceae) from different habitats". Proceedings for Natural Sciences 102 (2002): 59-70.
- Sahu PK., et al. “Evaluation of yield components in mulberry (Morus ) germplasm varieties". Acta Botanica Indica 23 (1995): 191-195.
- Vijayan K., et al. "Correlation studies in mulberry (Morus)". Indian Journal of Genetics and Plant Breeding 57 (1997): 455-460.
- Banerjee R., et al. "Genetic diversity and inter-relationship among mulberry genotypes". Journal of Genetics and Genomics 34 (2007): 691-697.
- Das BC and S Krishnaswamy. “Estimation of compounds of variation of leaf yield and its related traits in mulberry". Journal of Sericultural Science of Japan 38 (1969): 242-248.
- Paul DC., et al. "Impact of dietary moisture on nutritional indices growth of Bombyx mori and concomitant larval duration". Journal of Insect Physiology 38 (1992): 229-235.