Abstract

  Virus species belonging to the genus Potyvirus are the most common viruses infecting sweet potato crop. Among these viruses, Sweet potato Feathery Mottle Virus (SPFMV) is the most damaging and widespread in the world. To assess the potyvirus disease on sweet potato in Burkina Faso, a total of 300 samples were collected from the nine largest sweet potato producing regions. Samples were analyzed using RT-PCR and products were Sequenced. Bioinformatic analyzes were performed to know the strains of the viruses. The results revealed that SPFMV is the main Potyvirus infecting sweet potato in Burkina Faso with a prevalence of about 28.33%. A total of seven isolates of SPFMV were successfully sequenced and used for phylogenetic analyzes. These isolates have shown 99% nucleotide identity with the phylogroup A-II (SPFMV-O), and some of them had 95% nucleotide identity with phylogroup B (SPFMV-RC). This study showed that SPFMV is the main Potyvirus-infecting sweet potato in Burkina Faso.

Keywords: Sweet potato; Potyvirus; Sweet Potato Feathery Mottle Virus; Burkina Faso

References

1. Kwak H-R., et al. "Molecular characterization of five potyviruses infecting Korean sweet potatoes based on analyses of complete genome sequences”. Plant Pathology Journal 31 (2015): 388-401.
2. Mukasa SB., et al. "Incidence of viruses and virus-like diseases of sweetpotato in Uganda”. Plant Disease 87 (2003): 329-335.
3. Clark CA., et al. "Sweetpotato viruses: 15 years of progress on understanding and managing complex diseases”. Plant Disease 96 (2012): 168-185.
4. Gadhave KR., et al. "Aphid Transmission of Potyvirus: The Largest Plant-Infecting RNA Virus Genus”. Viruses 12 (2020): 1-22.
5. Moury B and Desbiez C. “Host range evolution of potyviruses: A global phylogenetic analysis”. Viruses 12 (2020).
6. Oana D., et al. "Potyviridae Family - short review”. Journal of Horticulture, Forestry and Biotechnology 13 (2009): 410-421.
7. Tibiri BE., et al. "Effects of sweet potato feathery mottle virus, sweet potato chlorotic stunt virus and their co-infection on sweet potato yield in Western Burkina Faso”. Open Agriculture 4 (2019): 758-766.
8. Gibson RW., et al. "Symptoms, aetiology and serological analysis of sweet potato virus disease in Uganda”. Plant Pathology 47 (1998): 95-102.
9. Karyeija RF., et al. "Synergistic interactions of a Potyvirus and a phloem-limited Crinivirus in sweet potato plants”. Virology 36 (2000): 26-36.
10. Mukasa SB., et al. "Interactions between a Crinivirus, an Ipomovirus and a Potyvirus in co-infected sweetpotato plants”. Plant Pathology 55 (2006): 458-467.
11. Untiveros M., et al. "Molecular variability of sweet potato feathery mottle virus and other potyviruses infecting sweet potato in Peru”. Archives of Virology 153 (2008): 473-483.
12. Marie-Jeanne V., et al. "Differentiation of Poaceae potyviruses by reverse transcription-polymerase chain reaction and restriction analysis”. Journal of Phytopathology 148 (2000): 141-151.
13. Bao Y., et al. "Improvements to pairwise sequence comparison (PASC): a genome-based web tool for virus classification”. Archives of Virology 159 (2014): 3293-3304.
14. Kumar S., et al. "MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms”. Molecular Biology and Evolution 35 (2018): 1547-1549.
15. Tibiri EB., et al. "Characterisation of virus species associated with sweetpotato virus diseases in Burkina Faso”. Plant Pathology 69 (2020): 1003-1017.
16. Karyeija RF., et al. "The significance of sweet potato feathery mottle virus in subsistence sweet potato production in Africa”. Plant Disease 82 (1998): 1-15.
17. Loebenstein G. “Control of sweet potato virus diseases”. Advances in Virus Research 91 (2015): 33-45.
18. Prasanth G and Hegde V. “Occurrence of sweet potato feathery mottle virus and sweet potato leaf curl Georgia virus on sweet potato in India”. Plant Disease 92 (2008): 311.

Citation

Citation: Fidèle Tiendrébéogo., et al. "Characterization and Distribution of Potyvirus Species Infecting Sweet Potato (Ipomoea batatas) in Burkina Faso". Acta Scientific Agriculture 4.11 (2020): 53-57.

Copyright

Copyright: © 2020 Fidèle Tiendrébéogo., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.