Acta Scientific Agriculture (ASAG)(ISSN: 2581-365X)

Research Article Volume 4 Issue 7

Effect of Plant Growth Promoting Rhizobacteria on Soil Profile, Growth Physiology and Antioxidant Activity of Stevia rebaudiana Bertoni

Shashi Kant Shukla1*, S Krithika2, Shraddha Shukla3 and Anupam Dikshit4

1Anupam Rural Laboratory, Centre of Science and Society, IIDS, University of Allahabad, Allahabad, India
2Brandenburg University of Technology (BTU) Cottbus-Senftenberg, Germany
3Nanotechnology Application Centre, University of Allahabad, Allahabad, India
4Biological Product laboratory, Botany Department, University of Allahabad, Allahabad, India

*Corresponding Author: Shashi Kant Shukla, Anupam Rural Laboratory, Centre of Science and Society, IIDS, University of Allahabad, Allahabad, India.

Received: April 15, 2020; Published: June 08, 2020



 The present study was performed with the main objective of investigation of enhancement in the growth and antioxidant activity of Stevia rebaudiana, Bertoni, a medicinal plant and chief source of stevioside, natural sweetener. The whole experiment was followed by macro and micronutrients analysis of used soil for pot experiment. In the analysed concentration ratio; potassium was greater than and phosphorous. So, there was a need to dissolve these nutrients into the usable form; Paenibacillus polymyxa was selected due to having these characteristics. The results, observed in 0, 10 and 20 days, showed that in comparison to control, inoculation with experimented soil bacterium, considerably increased root and shoot length as well as no and size of leaves in plant. The positive impact of the performed experiments was obtained after 10 and 20 days respectively. Furthermore, antioxidant efficacy of S. rebaudiana was done using DPPH method which confirms the plant to be free radical scavenger. The conclusion of the experiment showed the efficacy of P. polymyxa on escalation of Stevia.

Keywords: Shashi Kant Shukla, Anupam Rural Laboratory, Centre of Science and Society, IIDS, University of Allahabad, Allahabad, India.



  1. Shukla SK., et al. “Bio-Sweetener (Stevia rebaudiana Bertoni): Boon for rural India”. Organic and Medicinal Chemistry International Journal (2017): 03.
  2. Salerno G and Curatti L. “Origin of sucrose metabolism in higher plants: when, how and why?” Trends in Plant Science 8 (2003): 63-69.
  3. Goyal SK., et al. “Stevia (Stevia rebaudiana) a bio-sweetener: a review”. International Journal of Food Sciences and Nutrition1 (2010): 1-10.
  4. Shukla SK., et al. “Prediction and validation of Gold nanoparticles on plant growth promoting rhizobacteria (PGPRs): a step towards development of nano-biofertilizers”. Nanotechnology Reviews (2015).
  5. Shukla SK., et al. “Impact of eco-friendly soil microbe on Macrotyloma uniflorum (lam.) Verdc: a medicinal pulse”. International Journal of Life science and Pharma Reviews 7 (2017): 2.
  6. Shukla SK., et al. “Impact of Pseudomonas putida on the growth parameters of Fenugreek (Trigonella foenum graecum): a traditional herbal boon for rural population”. Science and Technology 5 (2017): 2.
  7. Shukla SK. “Soil improvement and Pulses: Healthy Rural India”. Global Journal of Bio-Science and Biotechnology 6 (2017): 04.
  8. Dikshit A., et al. “Plant growth promotion by plant growth promoting rhizobacteria (PGPR) with nanomaterials in current agricultural scenario”. Lap Lambert Academic Publishing (2013): 978-3-659-36774-8.
  9. Adesemoye AO., et al. “Comparison of plant growth-promotion with Pseudomonas aeruginosa and Bacillus subtilis in three vegetables”. Brazilian Journal of Microbiology 39 (2008)a: 423-426.
  10. Adesemoye AO and Kloepper JW. “Plant-microbes interactions in enhanced fertilizer use efficiency”. Applied Microbiology and Biotechnology 85 (2009): 1-12.
  11. Compant SW., et al. “Use of plant growth-promoting bacteria for bio-control of plant diseases: principles, mechanisms of action, and future prospects”. Applied and Environmental Microbiology 71 (2005): 4951-4959.
  12. Kloepper JW., et al. “Plant root-bacterial interactions in biological control of soil borne diseases and potential extension to systemic and foliar diseases”. Australasian Plant Pathology 28 (1999): 21-26.
  13. Sundara-Rao WVB and Sinha MK. “Phosphate dissolving micro-organisms in the soil and rhizosphere Indian”. The Journal of Agricultural Science 33 (1963): 272-278.
  14. Sindhu SS., et al. “Antagonistic effect of Pseudomonas spp. on pathogenic fungi and enhancement of plant growth in green gram (Vigna radiata)”. Biology and Fertility of Soils 29 (1999): 62-68.
  15. Clinical and Laboratory Standards Institute, Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically; Approved Standard-Seventh Edition. CLSI Document (2008): M7-A7.
  16. Marsden SB. “Antioxidant Determinations by the Use of a Stable Free Radical”. Nature 181 (1958): 1199-1200.
  17. Hilda R and Reynaldo F. “Phosphate solubilizing bacteria and their role in plant growth promotion”. Biotechnology Advances 17 (1999): 319-339.
  18. Priyanka P and Sindhu SS. “Potassium Solubilization by Rhizosphere Bacteria: Influence of Nutritional and Environmental Conditions”. Journal of Microbiology Research1 (2013): 25-23.
  19. Wu SC., et al. “Effects of biofertilizer containing N-fixer, P and K solubilizers and AM fungi on maize growth: a greenhouse trial”. Geoderma 125 (2005): 155-166.
  20. Moumita D., et al. “Plant growth promoting rhizobacteria enhance growth and yield of chilli (Capsicum annuum L.) under field conditions”. Australian Journal of Crop Science5 (2011): 531-536.
  21. Vani T., et al. “Antioxidant properties of the ayurvedic formulation triphala and its constituents”. Pharmaceutical Biology 35 (1997): 313-317.


Citation: Shashi Kant Shukla., et al. “Effect of Plant Growth Promoting Rhizobacteria on Soil Profile, Growth Physiology and Antioxidant Activity of Stevia rebaudiana Bertoni". Acta Scientific Agriculture 4.6 (2020): 27-32.


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