C Kipgen and VK Khanna*
School of Crop Improvement, College of Post-Graduate Studies, Central Agricultural University, Umiam, Meghalaya, India
*Corresponding Author: VK Khanna, School of Crop Improvement, College of Post-Graduate Studies, Central Agricultural University, Umiam, Meghalaya, India.
Received: August 03, 2018; Published: August 09, 2018
Citation: C Kipgen and VK Khanna. “Studies on Hybridization and Genetic Diversity in Cucumber (Cucumis sativus L.)". Acta Scientific Agriculture 2.9 (2018).
Crossability of various accessions of cucumber on the basis of pollen development and fruit set was studied along with genetic diversity by SSR markers. There was a non-significant correlation between fruit set and percent viable pollen. In all selfings and the crosses, pollen germination increased from 1 hour to 4 hours after pollination. Pollen germination had a highly significant correlation with fruit set. On selfing, the fruit set was positively correlated with faster pollen tube growth whereas it was not so in the case of crosses. There was a positive correlation between pollen tube growth and fruit set. Nineteen SSR primers were selected to assess genetic diversity of 35 accessions of cucumber. The polymorphic SSR primers generated a total of 33 alleles which ranged from 2 to 4 with an average of 2.53 per locus. Cluster analysis performed based on Bray Curtis similarity matrix showed that highest dissimilarity was found in cluster I which comprised of only one accession, IIHR-76 which is distinct from the rest of the accessions. The value of Bray-Curtis similarity coefficient ranged from 33 to 100 with the average value of 65. In the principal component axes, the first component PCA plot explained 22% variation, whereas the second and the third component explained 15.1% and 13.4% variation, respectively. The grouping obtained through PCA was comparable to Bray Curtis similarity matrix cluster analysis.
Keywords: Crossability; Cucumis sativus; Pollen Tube; Fruit Set; SSR
Copyright: © 2018 C Kipgen and VK Khanna. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.