The Identification and Quantitation of Components in Essential Oils of Citrus kinokuni Tanaka Peel

Zheng Pan^1,2, Ting-Li Han^3,4 and Jian Wang^1,2*

¹Department of Chinese Medicine and Chinese Material Medica, Chongqing Medical University, Chongqing China
²Chongqing Key Laboratory of Traditional Chinese Medicine for Prevention and Cure of Metabolic Diseases, Chongqing, China
³First Affiliated Hospital of Chongqing Medical University, Chongqing, China
⁴Liggins Institute, University of Auckland, Auckland, New Zealand

*Corresponding Author: Jian Wang, Department of Chinese medicine and Chinese Materia Medica (CMM), Chongqing Medical University, Chongqing, China.

Received: June 14, 2018; Published: July 31, 2018

Citation: : Jian Wang., et al. “The Identification and Quantitation of Components in Essential Oils of Citrus kinokuni Tanaka Peel". Acta Scientific Agriculture 2.8 (2018).

Abstract

  The extracted essential oils from the peels of Citrus kinokuni Tanaka collected from the same plant grown in Nancheng town, Fuzhou city, Jiangxi province of China in different time, respectively, were separated by column chromatography through different eluents. The chemical compositions in the essential oils and its separated parts were investigated by gas chromatography-mass spectrometry (GC–MS). The Automated Mass Spectral Deconvolution and Identification System (AMDIS) combined in the GC-MS chemstation was used to deconvolve the overlapped or even embedded peaks in the total ion chromatogram (TIC) obtained by GCMS, and to extract the characteristic ion peaks of corresponding compound. A total of 183 compounds were quantified in which 109 components were identified and 74 components with unknown identities were detected. The essential oils from C. kinokuni Tanaka peel were high in β-pinene, p-cymene, d-limonene, γ-terpinene, α-terpineol, linalool, thymol, (E,E)-α-farnesene, spathulenol, and (-)-spathulenol. At the same time, the chemotype of the essential oils was analyzed, which showed (E,E)-α-farnesene played an important role in differentiating the essential oils from the peel of C. kinokuni Tanaka from that of other varieties of C. reticulata Blanco. The study demonstrated the separation of essential oils by column chromatography through suitable eluents can differentiate the components effectively. In sequence, they are the hydrocarbon terpenes, the other oxygenated terpenes but not the alcohol of terpenes, the alcohol of terpenes, and acids, respectively.

Keywords: Citrus kinokuni Tanaka; Citri Pericarpium Reticulatae; Citri Pericarpium Reticulatae Viride; Essential Oil; Automated Mass Spectral Deconvolution and Identification System (AMDIS); Chemotype

Copyright: © 2018 Jian Wang., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.