Narges Atabaki1,2, Rosimah Nulit3*, Nahid Kalhori3, Nazerita Lasumin3, Mahbod Sahebi4 and Rambod Abiri2
1slamic Azad University, Science and Research Branch, Tehran, Iran
2Department of Biochemistry, Faculty of Biotechnology and Molecular Science, Universiti Putra Malaysia, Selangor, Malaysia
3Department of Biology, Faculty of Science, Universiti Putra Malaysia, Selangor, Malaysia
4Laboratory of Food Crops, Institute of Tropical Agriculture, Universiti Putra Malaysia, Selangor, Malaysia
*Corresponding Author: Rosimah Nulit, Department of Biology, Faculty of Science, Universiti Putra Malaysia, Selangor, Malaysia.
Received: May 12, 2018; Published: July 02, 2018
Citation: Rosimah Nulit., et al. “In vitro Selection and Development of Malaysian Salt-Tolerant Rice (Oryza sativa L. cv. MR263) Under Salinity". Acta Scientific Agriculture 2.8 (2018).
An efficient plant regeneration protocol via somatic embryogenesis from Oryza sativa L. cv. MR263 was established in this study. MR263 seeds were cultured on MS media supplemented with ideal concentration of 2, 4-D, 1 mg/L for 30 days to produce callus. Then, callus was subjected to different concentration of NaCl (0, 100, 150, 200, and 300 mM) on the same MS media to produce salttolerant callus. Four months old NaCl-treated and NaCl-free callus have been screened based on the characteristics such as physiological, morphological and biochemical markers to select salt-tolerant line. As a result, callus treated with low concentration of NaCl showed yellow color and friable proliferating, however callus treated with 200 and 300 mM NaCl shows more watery, discoloration with brown patch. To induce shoot and root, selected salt-tolerant callus was sub-cultured on MS media containing auxin and kinetin. The proline content, total soluble sugar and total protein were enhanced significantly in NaCl-treated callus. At cellular level, histological analysis of salt-tolerant MR263 callus revealed that salinity negatively affected on development somatic embryos. Therefore of cellular responses and growth performance, the plantlets that regenerated from 100 mM NaCl-treated callus were selected as salttolerant lines. This research suggests a novel protocol for regeneration and development salt-tolerant rice cultivar and other crops.
Keywords: Salinity; Indica Rice MR263; Regeneration; Proline Content; Salt- Tolerant Callus; Salt- Tolerant MR263
Copyright: © 2018 Rosimah Nulit., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.