To Standardize the Optimum Concentration of Auxins (NAA and IBA) for Better Rooting of Shoots Under In Vitro Conditions

T Navya swetha¹*, A Girwani², A Manohar Rao³ andP Saidaiah⁴

¹Floriculture and Landscaping Architecture, Sri Konda Laxman Telangana State Horticultural University, Budwel, Hyderabad, Telangana
²Floriculture and Research Station, Sri Konda Laxman Telangana State Horticultural University, Budwel, Hyderabad, Telangana
³Department of Horticulture, College of Agriculture, Professor Jayashankar Telangana State Agricultural University, Rajendranagar, Hyderabad, Telangana
⁴Genetics and Plant Breeding, College of Horticulture, Sri Konda Laxman Telangana State Horticultural University, Budwel, Hyderabad,Telangana

*Corresponding Author: T Navya swetha, Floriculture and Landscaping Architecture, Sri Konda Laxman Telangana State Horticultural University, Budwel, Hyderabad, Telangana.

Received: April 17, 2018; Published: June 11, 2018

Citation: T Navya swetha., et al. “To Standardize the Optimum Concentration of Auxins (NAA and IBA) for Better Rooting of Shoots Under In Vitro Conditions". Acta Scientific Agriculture 2.7 (2018).

Abstract

  The present investigation was carried out at “AGRI-BIOTECH FOUNDATION” in PJTSAU campus and College of Horticulture, Rajendranagar, Hyderabad during the year 2015 - 2016. The present study was conducted under four experiments for regeneration of capitulum explants in Murashige and Skoog medium supplemented with different hormonal concentrations with an objective to determine the best concentration of growth regulators for culture establishment, shoots multiplication, rooting of in vitro shoots and further to standardize suitable hardening media for establishment of gerbera plants. In the third experiment for standardization of basal media for in vitro rooting, seven treatments were formulated with MS basal medium (control), MS medium + IBA, MS medium + NAA at different concentrations. Highest per cent rooting (83.33%), earlier rooting (19.00) with maximum root length (54.66 mm) was obtained in MS medium supplemented with 2 mg/L IBA.

Keywords: NAA; IBA; Rooting; Shoots

Copyright: © 2018 T Navya swetha., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.