Effect of Chemical and Biological Treatment for the Control Seed-Borne Mycoflora of Barley (Hordeum vulgare L.)

Lukman Ahmad* and Razia K Zaidi

Section of Plant Pathology, Department of Botany, Aligarh Muslim University, Aligarh, India

*Corresponding Author:Lukman Ahmad, Section of Plant Pathology, Department of Botany, Aligarh Muslim University, Aligarh, India.

Received: April 10, 2018; Published: May 09, 2018

Citation: Lukman Ahmad and Razia K Zaidi. “Effect of Chemical and Biological Treatment for the Control Seed-Borne Mycoflora of Barley (Hordeum vulgare L.)". Acta Scientific Agriculture 2.6 (2018).

Abstract

  Dry seed inspection showed that seed abnormalities and conidial fructifications were observed under naked eye and stereobinocular microscope. Seed health techniques confirmed the most frequently isolated fungus was Alternaria alternata followed by Rhizopus stolonifer, Mucor spp., Fusarium moniliforme and Aspergillus flavus determined by seed plating on both standard blotter method and agar plate method respectively. For the control of seed-borne fungi fungicides viz. Mancozeb, Carbendazim and Mancozeb plus Carbendazim mixture as well as bioagents Trichoderma viride, Trichoderma harzianum and Aspergillus niger were tested through poisoned food and dual culture technique respectively. The results indicated that different concentrations of fungicides and bioagents were found significantly effective in percent growth inhibition of A. alternata, R. stolonifer, Mucor spp., F. moniliforme and A. flavus. Among fungicides, Mancozeb plus Carbendazim mixture was found highest growth inhibition at 300 ppm concentration followed by Mancozeb and Carbendazim alone over control. However, in biocontrol agent’s highest percent growth inhibition was observed in T. viride followed by T. harzianum and A. niger as compared to control. Further seed priming with Mancozeb plus Carbendazim and T. viride was also found to be ffective in eliminating seed-borne fungi and results percent germination increase in both agar plate method and blotter method over control. During pot experiment treated seeds were found significantly effective in increase plant growth and chlorophyll content over control plants.

Keywords: Biocontrol Agents; Fungicides; Seed Inspection; Seed-Borne Fungi; Seed Treatment

Copyright: © 2018 Lukman Ahmad and Razia K Zaidi. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.