Acta Scientific Agriculture

Research ArticleVolume 2 Issue 5

Green Synthesis of Silver Nanoparticle by Acalypha indica and its Antifungal Effect against Phytopathogen Colletotrichum capsici

Rajyasri Ghosh1*, Sushmita Dutta2 and Shampa Bhattacharyya1

1Associate Professor, Post Graduate Department of Botany, Scottish Church College, Kolkata, India
2M.Sc Student, Post Graduate Department of Botany, Scottish Church College, Kolkata, India

*Corresponding Author: Rajyasri Ghosh, Associate Professor, Post Graduate Department of Botany, Scottish Church College, Kolkata, India.

Received: February 19, 2018; Published: April 16, 2018

Citation: Rajyasri Ghosh., et al. “Green Synthesis of Silver Nanoparticle by Acalypha indica and its Antifungal Effect against Phytopathogen Colletotrichum capsici". Acta Scientific Agriculture 2.5 (2018).

Abstract

  In the present investigation green synthesis of silver nanoparticles was carried out using aqueous leaf extract of Acalypha indica. The formation of nanosilver was confirmed by UV-Vis Spectroscopy. The absorbance peak at 450nm indicated the presence of silver nanoparticles in the solution. The antifungal property of silver nanoparticles was evaluated against pathogenic fungus Colletotrichum capsici both in vitro and in field inoculation experiment. Application of silver nanoparticles produced significant inhibition of growth of fungal mycelia as well as conidial germination in vitro . It has been observed that about 54% and 70% reduction in mycelia growth took place at 100 ppm and 200 ppm concentrations of silver nanoparticles respectively. Results of spore germination test revealed about 97% reduction in germination at 10 ppm concentration of silver nanoparticles. In the field trial experiment significant reduction (46%) in anthracnose disease of bean caused by C. capsici was observed when the plants were treated with 200 ppm of nanosilver prior to inoculation. Results of the present study highlighted the eco-friendly approach of plant mediated synthesis of nanosilver and its potential application in the field as an alternative to chemical fungicides for disease management.

Keywords: Acalypha indica; Silver Nanoparticle; Bean Anthracnose; Colletotrichum capsici; Antifungal Property

Copyright: © 2018 Rajyasri Ghosh., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.



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