Abstract

Background: Essential oils (EO_S) possess a wide range of significant properties including antiphlogistic, spasmolytic and antinociceptive effects. In this study, we use essential oils (EO_S) from Thymus daenensis, Thymus caramanicus and Ziziphora clinopodioides.

Purpose of the Study: This study attempts to determine the growth inhibition level of the essential oils of three plants against Aspergillus flavus and Aspergillus parasiticus for 14 days.

Results: The highest rate of inhibition was observed in Thymus daenensis in concentration above 7 μL in 100 mL of PDA medium in which no growth was observed during 14 days.

The Main Findings: Among the three essential oils, T. daenensis contains the highest level of thymol (77.62%). Ziziphora clinopodioides contains pulegone (31.21%), menth-3-en-8-0l (23.82%), menthol (7.21%), borneol (2.25%), carvacrol (5.38%) and piperitone (5.55%). Only a concentration of 9 μL of essential oils of Z. clinopodioides can prevent mycelium growth of both fungi for 7 days. Thymus caramanicus contains carvacrol (65.52%), p-cymene (13.21%), gamma-terpinene (4.44%), thymol (4.14%) and linalool (2.63).

Conclusion: Although T. caramanicus contains 65.52% carvacrol, its inhibition growth ability does not reach 100% in all concentrations and it is capable of inhibiting fungal growth completely (100%) at 7 and 9 μL concentrations for one day. This indicates that compound thymol is more effective than carvacrol in prevent of growth fungi.

Keywords: Essential Oils; Aspergillus flavus; Aspergillus parasiticus; Natural Antifungal

Copyright: © 2018 Khorasani S., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.