Comparative Analysis in CMA Banding Patterns Between Wild and Cultivated Citrus Species of North-East India

Marlykynti Hynniewta¹, Banraplang Kharshandi¹, Sayoowan Pala¹, Surendra Kumar Malik² and Satyawada Rama Rao¹*

¹Plant Biotechnology Laboratory, Department of Biotechnology and Bioinformatics, North-Eastern Hill University, Shillong, Meghalaya, India
²Tissue Culture and Cryopreservation Unit, National Bureau of Plant Genetic Resources, New Delhi, India

*Corresponding Author: Satyawada Rama Rao, Plant Biotechnology Laboratory, Department of Biotechnology and Bioinformatics, North-Eastern Hill University, Shillong, Meghalaya, India.

Received: February 14, 2018; Published: March 23, 2018

Citation: Satyawada Rama Rao., et al. “Comparative Analysis in CMA Banding Patterns Between Wild and Cultivated Citrus Species of North-East India". Acta Scientific Agriculture 2.4 (2018).

Abstract

  Fluorochrome staining with chromomycin A3 (CMA) was used to characterize the CMA banding pattern of chromosomes of 5 wild and 5 cultivated species of Citrus collected from North-east India. All species used in this study had 2n = 18 chromosomes. These chromosomes were classified into five types based on the number and position of CMA-positive bands; B: one telomeric and one proximal band, C: two telomeric bands, D: one telomeric band, E: without bands and Dst: type D with a satellite chromosome. Each species possessed three to five types of chromosomes and unique CMA banding pattern. It was exciting to note the presence of type D chromosomes of C. maxima and C. ichangensis with satellite body. The taxonomical position of the true basal species viz. C. medica, C. reticulata and C. maxima is further confirmed by their identical configurations in the CMA banding pattern. The CMA banding pattern for C. ichangensis (Papeda) shows close relations to the sub-genera Citrus. On the other hand the banding patterns in the wild species C. indica and C. assamensis has indicated their genomic hybridity in relation to the true basal species.

Keywords: Chromomycin A3; Karyotype; Citrus; Wild; North-East; Heterochromatin; Wild

Copyright: © 2018 Satyawada Rama Rao., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.