Pollen Grain Mediated Gene Transfer for Efficient Plant Transformation

Subramanian Sankaranarayanan*

Institute of Transformative Bio-Molecules (WPI-ITbM), Nagoya University, Japan

*Corresponding Author: Subramanian Sankaranarayanan, Institute of Transformative Bio-molecules (WPI-ITbM), Nagoya University, Nagoya, Japan.

Received: January 11, 2018; Published: February 05, 2018

Citation: Subramanian Sankaranarayanan. “Pollen Grain Mediated Gene Transfer for Efficient Plant Transformation". Acta Scientific Agriculture 2.3 (2018).

  Plant transformation has become an integral part of crop improvement programs, for breeding new varieties of crops with higher yields and resilience to different stress conditions [1]. However, there are several challenges that have to be overcome to create genetically modified (GM) crops. The most challenging part is the recovery of a transgenic event, which depends on our ability to regenerate a new plant from a single transformed cell and killing off all the non‐transformed cells surrounding it [2]. Most of existing transformation methods rely on a sophisticated tissue culture based protocol (organogenesis or somatic embryogenesis) for regeneration of the transgenic plant, that can be long, complicated and labor intensive [3,4]. Plant cells from different species and even cultivars of the same species respond very differently in the standard tissue culture media, making the regeneration procedure challenging [3]. Development of alternate, and simple transformation protocols can eliminate the need for tissue culture and regeneration. As of now, the model weed plant, Arabidopsis is the only plant species for which a rapid transformation method called ‘floral dip’ exists [5]. Most plant species have a closed gynoecium, while Arabidopsis flowers have an open gynoecium that allows, the soil bacterium Agrobacterium tumefaciens, to infect the ovules deliver the recombinant DNA into the egg [6].

Copyright: © 2018 Subramanian Sankaranarayanan. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.