Kritima Kapoor*, Opinder Singh and Devender Pathak

Department of Veterinary Anatomy, College of Veterinary Sciences, Ludhiana, GADVASU Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, India

*Corresponding Author: Kritima Kapoor, Department of Veterinary Anatomy, College of Veterinary Sciences, Ludhiana, GADVASU Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, India.

Received: June 24, 2022; Published: July 13, 2022

Abstract

The current study was conducted on corpus luteum (CL) from healthy buffalo ovaries (n = 40) collected from local slaughterhouses. The CL was cleaned, observed grossly, and categorized into early luteal (stage I, 1 to 5 days), mid-luteal (stage II, 6 to 11 days), late luteal (stage III, 12 to 16 days), and regressing phase (stage IV, 17 to 20 days) based on their gross morphology, having ten ovaries in each group. The cryosections of CL obtained were incubated with substrates to study the distribution pattern of NADPH-diaphorase, NADH-diaphorase, and Non-specific esterase (NSE). The NADH-diaphorase activity was moderate within the developing luteal cells and weak in connective tissue in corpus haemorrhagicum, with strong to intense reaction in the luteal cells of mid-luteal phase CL and negligible activity in luteal cells of regressing phase. However, luteal cells of mid-luteal phase CL exhibited mixed activity for NADPH-d. In the corpus albicans phase, the NADPH-d activity was almost nil. NSE activity was moderate in the developing luteal cells in the corpus haemorrhagicum phase, strong in the luteal cells in mid-luteal phase, and moderate to weak activity for NSE within the cytoplasm of the regressing luteal cells. Therefore, it can be concluded that increased NADPH during the mid-luteal phase might be correlated to the increased secretory activity of cells as the enzyme is responsible for the conversion of cholesterol to progesterone and fatty acid synthesis. Histoenzymic localization of diaphorases and esterases in cyclic CL of buffalo, therefore, indicated their subsequent role in steroidogenesis.

Keywords: Buffalo; Corpus Luteum; Diaphorases; Esterases; Histoenzyme; Reproduction

References

1. Smith GW and Meidan R. “Ever-changing cell interactions during the life span of the corpus luteum: Relevance to luteal regression”. Reproductive Biology 14 (2014): 75-82.
2. Kapoor K., et al. “Lipid Distribution Variations in Different Stages of Cyclic Corpus Luteum of Indian Buffalo”. Journal of Animal Research3 (2018): 379-385.
3. Kapoor K., et al. “Immunoexpression of cytokine tumour necrosis factor-α suggesting its role in formation and regression of corpus luteum in Indian buffalo”. Reproduction in Domestic Animals (2020): 1-11.
4. Petculescu-Ciochina L., et al. “Cytohistological Aspects of Apoptosis in Luteal Regression in Mice”. Scientific Papers: Animal Science and Biotechnologies2 (2013): 215-220.
5. Anderson LL and Melampy RM. "Mechanisms Controlling the Formation and Persistence of the Corpus Luteum". Publications from USDA-ARS/UNL Faculty 738 (1965): 68.
6. Trivedi S and Lall SB. “Ovarian dehydrogenases of the non-pregnant, pregnant and lactating Rhinopoma microphyllum kinneari (Chiroptera: Rhinopomatidae)”. Vespertilio 8 (2004): 105-112.
7. Pathak D and Bansal N. “Gross morphological studies on hypothalamo-hypophyseal-ovarian axis of Indian buffalo”. Ruminant Science2 (2018): 137-143.
8. Kapoor K and Singh O. “Histoenzymic Distribution in Ileal Peyer’s Patches of Buffalo during Prenatal Development”. Journal of Animal Research4 (2016): 677-683.
9. Pearse AGE. “Histochemistry: Theoritical and Applied. 3^rd edition”. Vol II, Churchill Livingstone, London (1972).
10. Barka T and Anderson PJ. “Histochemistry: Theory, Practice and Bibliography”. Harper and Row Publishers, Inc, New York (1963): 237-316.
11. Saidapur SK and Greenwald GS. “Sites of Steroid Synthesis in the Ovary of the Cyclic Hamster: A Histochemical Study”. American Journal of Anatomy 151 (1978): 71-86.
12. Miyamoto H and Ishibashi T. “Histochemical activities of hydroxysteroid dehydrogenase and lipids in the goat corpus luteum during late pregnancy”. Japanese journal Zootechnical Science2 (1982): 93-98.
13. Singh O and Roy KS. “Histoenzymic studies on oxidoreductases in cyclic corpus luteum of the Indian buffalo”. Buffalo Journal 3 (1996): 399-403.
14. Sasaki Y and Hanson GC. “Glucose oxidation in corpus luteum and the concentration of sex steroids in systemic plasma during the reproductive cycle of the guinea pig”. Endocrinology5 (1974): 1213-1218.
15. Sorensen VW and Singh UB. “On mitochondrial inclusions in granulose lutein cells of pregnant cows”. Experientia 29 (1973): 592.
16. Hoyer PE. “Hitoenzymology of the human ovary: dehydrogenases directly involved in steroidogenesis”. In: The Ovary (Eds), P M Motta and Hafez ESE. Martinus Nijhoff publishers, The Hauge (1980): 52-67.
17. Boos A. “Enzyme histochemistry of bovine luteinized follicular cysts and corpora lutea of estrus cycle”. Zuchthygiene 23 (1988): 65-77.
18. Roy KS and Saigal RP. “Histoenzymological study of oxidoreductases and acetylcholinesterase in the pregnant sheep ovary”. Indian Journal of Animal Science 55 (1985): 730-733.
19. Hunter RL and Kneiske KM. “Quantitative study of nonspecific esterases in the rat ovary”. Journal of Histochemistry and Cytochemistry 5 (1957): 154-158.
20. Chahal K., et al. “Histoenzymic localization of phosphatases, oxidoreductases and nonspecific esterase in the uterus of canines collected after ovariohysterectomy”. Acta Scientific Veterinary Sciences 6 (2022): 75-83.
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Citation

Citation: Kritima Kapoor., et al. “Histoenzymic Studies on Localization of Diaphorases and Esterases in Cyclic Corpus Luteum of Indian Buffalo".Acta Scientific Veterinary Sciences 4.8 (2022): 79-85.

Copyright

Copyright: © 2022 Kritima Kapoor., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.