Baidyanath Mishra¹*, Dharmendra Kumar², Srinivasa Reddy Yathapu³, Varun Kumar Singh², Aleena Gauri³ and Apratim Sai Rajesh³

¹Head, Department of Health Care R&D, Dabur Research and Development Centre, Dabur India Limited, Ghaziabad, Uttar Pradesh, India
²Department of Ayurveda Formulation, Dabur Research and Development Centre (Healthcare Divn.), Dabur India Limited, Ghaziabad, Uttar Pradesh, India
³Department of Medical Affairs and Clinical Research, Dabur Research and Development Centre (Healthcare Divn.), Dabur India Limited, Ghaziabad, Uttar Pradesh, India

*Corresponding Author: Baidyanath Mishra, Head, Department of Health Care R&D, Dabur Research and Development Centre, Dabur India Limited, Ghaziabad, Uttar Pradesh, India./p>

Received: April 16, 2026 Published: May 11, 2026

Abstract

The traditional preparations were developed over a period with combination of strong theoretical knowledge of natural ingredients and unique preparation processes. The Indian Ayurvedic text has plethora of such preparations as therapeutics, health and food supplement to support the ‘quality of life’ of human being. Khajurprash, an extention of Chyawanprash under proprietary category for achieving Rasayana(rejuvenation) has been meticulously prepared, possessing the Date palm fruit (Phoenix dactylifera L.), Amalaki (Emblica officinalis) and a combination of nearly 40 variety of herbs/spices. Dabur Khajurprash (D-KP) has been evaluated for its antioxidant, immunomodulatory and innate immune response induction property using the in vitro and ex vivo system. The antioxidant potential of D-KP was assessed in HepG2 cell for GSH levels, immunomodulatory activity by splenocyte TNF-α secretion, innate immunity response by NK cell function and phagocytosis by RAW 264.7 cultured cells. The results suggest that D-KP has antioxidant activity, assessed by reduced form of GSH levels, and was at par with Quercetin. A concentration dependent increase of TNF-α secretion by splenocytes up to 100 μg/mL pf D-KP and reduction of TNF-α levels with further higher concentrations of D-KP suggest the immunomodulatory potential. The D-KP has significantly increased the NK cell mediated YAC-1 cell lysis and phagocytosis assessed using the RAW 264.7 cells. Results of current study conclude that D-KP has antioxidant, immunomodulatory and innate immune response stimulation potential.

Keywords: Khajurprash; Chyawanprash; Antioxidant; Immunomodulatory; Innate Immune Response

Citation

Citation: Baidyanath Mishra., et al. “In vitro Evaluation of Antioxidant and Immunomodulatory Activity of Khajurprash: A Traditional Health Supplement Preparation". Acta Scientific Pharmaceutical Sciences 10.5 (2026): 16-23.

Copyright

Copyright: © 2026 Baidyanath Mishra., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.