Arcady Yu Shpachuk*, Nikita D Ponomarev, Elizaveta N Khryachkova and Vera A Kolodyaznaya

Scientific and Educational Center of Biotechnology and Bioengineering, Saint-Petersburg State Chemical-Pharmaceutical University, Russian Federation

*Corresponding Author: Arcady Yu Shpachuk, Scientific and Educational Center of Biotechnology and Bioengineering, Saint-Petersburg State Chemical-Pharmaceutical University, Russian Federation.

Received: July 22, 2025; Published: July 30, 2025

Abstract

This work presents a comparative analysis of various sources of organic nitrogen in terms of protein and amino nitrogen content in them for the cultivation of Streptomyces lavendulae in order to produce cholesterol oxidase. The possibility of replacing the traditional component of nutrient media, baking yeast, with alternative sources of organic nitrogen, such as yeast hydrolysate, autolysate, and extract, is being studied. An experiment was conducted to determine the influence of various components of the medium and cultivation conditions on the synthesis efficiency. The negative effect of some yeast substitutes on the enzyme activity has been established. The main parameters for optimizing the nutrient medium and obtaining the maximum amount of cholesterol oxidase have been determined. Among the factors studied, the concentration of the organic nitrogen source, glucose content, cultivation time, and vitamin B supplementation are the most important.

Keywords: Cholesterol Oxidase; Nutrient Medium; Organic Nitrogen Sources; Amino Nitrogen; Total Protein Content; Optimization; Plackett-Burman Design

References

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Citation

Citation: Arcady Yu Shpachuk., et al. “Selection of an Organic Nitrogen Source and Determination of the Parameters for Optimizing the Nutrient Medium for Culturing Streptomyces lavendulae with the Aim of Obtaining Cholesterol Oxidase". Acta Scientific Pharmaceutical Sciences 9.8 (2025): 29-32.

Copyright

Copyright: © 2025 Arcady Yu Shpachuk., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.