Shreyash P Gardi*, Sapna A Sharma, Kunal S Gaiakwad, Trushita S Bharaswadkar, Jayant T Funde and Piyush A Shirsathe

Student of Modern College of Arts Science and Commerce Ganeshkhind Pune

*Corresponding Author: Shreyash P Gardi, Student of Modern College of Arts Science and Commerce Ganeshkhind Pune.

Received: January 30, 2025; Published: February 05, 2025

Abstract

The present study explores an efficient in vitro regeneration protocol for Citrus aurantiifolia (acid lime) using axillary buds as explants. Acid lime is a commercially significant fruit crop, but its conventional propagation faces challenges due to its slow growth and susceptibility to diseases. In this context, tissue culture techniques offer a promising alternative for rapid and disease-free plant propagation. Axillary buds from mature, healthy acid lime plants were excised and cultured on Murashige and Skoog (MS) medium supplemented with various concentrations of plant growth regulators, including cytokinins and auxins, to optimize shoot proliferation and root induction. The highest regeneration rate was observed with MS medium containing 2.0 mg/L BAP (6-benzylaminopurine) and 0.5 mg/L NAA (naphthaleneacetic acid), resulting in efficient shoot multiplication. Rooting was successfully achieved on a medium with 1.0 mg/L IBA (indole-3-butyric acid). The regenerated plants were acclimatized and successfully transferred to the field with a high survival rate. This study demonstrates a reliable and reproducible method for acid lime micropropagation, which can be applied for large-scale commercial production and genetic improvement programs.

Keywords: Regeneration; Acid Lime; Axillary Bud

References

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Citation

Citation: Shreyash P Gardi., et al. “In vitro Regeneration of Acid Lime using Axillary Bud". Acta Scientific Pharmaceutical Sciences 9.3 (2025): 03-07.

Copyright

Copyright: © 2025 Shreyash P Gardi., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.