Afaf Mohammed Weli¹*, Khaled Ali Abdin¹, Mustafa Mazen Hamza¹, Jamaluddin Shaikh¹, Sadri Abdullah Said¹, Jamal Al-Sabahi³, Ajmal Khan², Saeed Allah², Abdullah Mohammed Al-Hatmi², Najeeb Ur Rahman², Salem Said Al Touby¹ and Ahmed Al-Harrasi¹

¹College of Health Sciences, University of Nizwa, Oman
²Natural and Medical Sciences Research Center, University of Nizwa, Oman
³Central Instrument Laboratory, College of Agricultural and Marine Sciences, Sultan Qaboos University, Oman

*Corresponding Author: Afaf Mohammed Weli, College of Health Sciences, University of Nizwa, Oman.

Received: May 23, 2024; Published: June 20, 2024

Abstract

The current study investigated the anti-microbial, enzymatic inhibition potential, anti-oxidative, and anti-depressive properties of the essential oil (EO) of peels of Citrus limetta Risso (Clp) plant. GC-MS analysis was performed to screen the Clp-EO active constituents. Limonene was found to be the main constituent of Clp-EO through NMR spectroscopic data. The Clp-EO was found to possess great anti-fungal inhibition of C. albicans (20 mm) as effective as the standard agent (Amphotericin B). On the other hand, its anti-bacterial activity was moderate at inhibiting S. aureus (8 mm) when compared to the standard (Ciprofloxacin). The antioxidant activity test was performed using DPPH radicals and the Clp-EO flourished strong antioxidant (90%) activity. Clp-EO exhibited substantial inhibition (IC50 = 28.13 ± 0.5 μg/mL) against prolyl endopeptidase, while notable inhibition (IC50 value of 62.52 ± 1.49 μg/mL) was observed in case of tyrosinase. Similarly, Clp-EO exhibited a strong inhibition (IC50, 38.58 ± 1.26 μg/mL) against α-glucosidase enzyme, while, in case of CA-II inhibitory assay, Clp-EO exhibited a notable inhibition (39.64%). Moreover, reductions in behavioral patterns in vivo indicated anxiolytic activity. These findings imply that Clp-EO could be a natural preservative in food or as an effective treatment against a variety of ailments.

Keywords: Citrus limetta Peels; Antioxidant; Antidiabetic; GC-MS; Enzyme Inhibition

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Citation

Citation: Afaf Mohammed Weli., et al. “Pharmacological and Phytochemical Screening of the Essential Oil from peels of Citrus limetta Risso".Acta Scientific Pharmaceutical Sciences 8.7 (2024): 72-80.

Copyright

Copyright: © 2024 Afaf Mohammed Weli., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.