Paras Kumar Prajapati¹*, Pankaj Mishra² and Pushpendra Kannojia³

¹Ph.D Research Scholar, BIU College of Pharmacy, Bareilly International University, Bareilly, UP, India
²Principal, Rohilkhand College of Pharmacy, Bareilly International University, Bareilly, UP, India
³Principal, BIU College of Pharmacy, Bareilly International University, Bareilly, UP, India

*Corresponding Author: Paras Kumar Prajapati, Ph.D Research Scholar, BIU College of Pharmacy, Bareilly International University, Bareilly, UP, India.

Received: May 09, 2024; Published: May 23, 2024

Abstract

The present study was based on the phytochemical investigation and isolation of active constituent from Hydro-alcoholic extract of Triticum aestivum (Wheat Grass). T. aestivum was grown under indoor conditions in Bareilly. The plant was authenticated by Dr. Alok Srivastava (Associate Professor) Plant Science. The authentication letter reference no. RU/PS/19/03 as issued from department of plant science MJP Rohilkhand University, Bareilly. Dried powdered leaves (50gm) and root (50gm) were extracted separately with Soxhlet apparatus using 500 ml of hydro-alcoholic solution (1:1) ethanol: water for 24 hrs. Using rotatory evaporator (≤40°C) the extract was dried. The dried extract of leaves and root was subjected to phytochemical investigation. The compound was isolated and characterized for the physicochemical and spectroscopy analysis. In results, upon calculation, the % yield for Triticum aestivum leaves and root extract was obtained as 54% and 18%, respectively. Upon column chromatography, NMR, FTIR, UV and Mass spectroscopy analysis the isolated compound in leaves extract was reported as 2,27-diamino-3,26-dihydroxyoctacosan-12-one. The melting point, molecular weight, molecular and Rf value were obtained as 82.6ºC, 470.7872, C28H58N2O3 and 1.46, respectively. In conclusion, it was found as amorphous, yellow-whiteand crystalline in nature with fish like odor. In future prospective, it suggests to isolate diverse phytochemicals for the treatment of various human illnesses. The active constituents could be utilized in suitable dosage form for better bioavailability and pharmacological effect.

Keywords: Triticum aestivum; Active Constituents; Isolation; Column Chromatography; Mass Spectroscopy

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Citation

Citation: Paras Kumar Prajapati., et al. “Phytochemical Investigation and Isolation of Active Constituent from Hydro-Alcoholic Extract of Triticum aestivum (Wheat Grass)"Acta Scientific Pharmaceutical Sciences 8.6 (2024): 89-96.

Copyright

Copyright: © 2024 Paras Kumar Prajapati., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.