Acta Scientific Pharmaceutical Sciences (ASPS)(ISSN: 2581-5423)

Research Article Volume 7 Issue 10

A Developed and Validated Method for the Determination of Zolmitriptan in Human Plasma Through Liquid Chromatography Mass Spectrometric (LC-MS/MS)

Poulami Sen1, Indrani Bhattacharyya2, Subhas Chandra Maity2, Souvik Chattopadhyay2, Debangshu Mishra2, Mrinmoy Nag3, Souvik Biswas4*, Biplab Debnath4 and Arijit Das4

1H.G.E.A. College of Pharmacy, Chas, Jharkhand, India
2Pandaveswar School of Pharmacy, Pandaveswar, West Bengal, India
3NEF College of Pharmaceutical Education and Research, Haibargaon, Assam, India
4Bharat Technology, Uluberia, West Bengal, India

*Corresponding Author: Souvik Biswas, Assistant Professor at Bharat Technology, Department of Pharmacology Uluberia, West Bengal, India.

Received: August 22, 2023; Published: September 13, 2023


A selective, rapid and sensitive liquid chromatography-mass spectrometry (LC-MS/MS) method was developed and validated for the quantitative estimation of zolmitriptan in plasma where Rizatriptan as internal standard and pretreatment of sample was involved a one-step extraction with ethyl acetate from plasma. Method: The sample was analyzed using Methanol: Buffer Solution in a ratio of 60:40, v/v as mobile phase at ambient condition and the volume of the sample was injected at 5 μl with run time of 2.5 mins. The chromatographic separation was achieved on a Chromolith® Speed ROD RP-18e 50 -4.6 mm column which was followed by detection with mass spectrometry. Results: The Retention time of the Zolmitriptan and Rizatriptan both have approximately 1.5 minutes with the Flow rate at 0.500 ml/minute. Linear calibration curves were obtained in the concentration range of 0.201 ng/ml to 14.873 ng/ml. The inter- and intra-day accuracy values were below 15% in the least internal control levels. Lipemic plasma ranged from 104.09% (HQC) to 104.97% (LQC) with a precision ranging from 3.79% (HQC) to 5.53% (LQC). The percent nominal of Zolmitriptan in Haemolysed plasma ranged from 105.03% (HQC) to 109.35% (LQC) with a precision ranging from 1.99% (LQC) to 3.06% (HQC). The mean% recovery of Drug A was 53.620% with a precision of 14.44%. The mean% recovery of internal standard Rizatriptan was 43.620% with a precision of 9.46%. Ruggedness was ranged from 91.38% (LLOQ QC) to 105.92% (LQC) with a precision ranging from 3.40% (MQC) to 7.24% (LLOQ QC). Therefore, all the validated parameters showed that the the method is specific, stable, accurate and precise. Conclusions: The results demonstrated that the above developed and validated LCMS method is simple, rapid, precise and accurate, which is useful for the estimation of Zolmitriptan in for the support to the pharmacokinetic study as well as pharmaceutical applications in the industry.

 Keywords: Liquid Chromatography Mass Spectroscopy; Antimigraine; Plasma; Zolmitriptan


  1. Goadsby PJ. “Emerging therapies for Migrains”. Nature Clinical Practice Neurology 3 (2007): 610-619.
  2. Kelman L. “The triggers or precipitants of the acute migraine attack”. Cephalalgia 5 (2007): 394-402.
  3. Turner L C., et al. “Migraine trigger factors in non-clinical Mexican-American population in San Diego County: Implications for etiology”. Cephalalgia6 (1995): 523-530.
  4. Schulte L H., et al. Photo-, osmo- and phonophobia in the premonitory phase of migraine: Mistaking symptoms for triggers?” The Journal of Headache and Pain 16 (2015).
  5. Andrea Negro., et al. “Serotonin receptor agonists in the acute treatment of migraine: a review on their therapeutic potential”. Journal of Pain Research 11 (2018): 515-526.
  6. Abram JA and Patel P. “Zolmitriptan”. In: StatPearls. Treasure Island (FL): StatPearls Publishing (2022).
  7. EJ Seaber., et al. British Journal of Clinical Pharmacology 46 (1998): 433.
  8. K Vishwanathan., et al. Rapid Communications in Mass Spectrometry 14 (2000): 168.
  9. ZJ Zhang., et al. Journal of Chromatography B 813 (2004): 227.
  10. DW Boulton., et al. Biomedical Chromatography 17 (2003): 48.
  11. JF Guo., et al. Biomedical Chromatography 20 (2006): 61.
  12. Watson JT and Sparkman OD. “Introduction to mass spectrometry: instrumentation, applications, and strategies for data interpretation”. The Atrium, Southern Gate, Chichester, West Sussex PO19 8SQ, England.: John Wiley and Sons (2007).


Citation: Souvik Biswas., et al. “A Developed and Validated Method for the Determination of Zolmitriptan in Human Plasma Through Liquid Chromatography Mass Spectrometric (LC-MS/MS)". Acta Scientific Pharmaceutical Sciences 7.10 (2023): 23-33.


Copyright: © 2023 Souvik Biswas., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


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