Marine-derived Producer Bor S17B13 and its Response to Variations in Salt (NaCl) Concentration and pH in the Growth Medium
Priyanka Sawant1, Kartik Vikani2 and Jignasha Thumar1*
1Department of Microbiology and Biotechnology, Gujarat University, Ahmedabad, Gujarat, India
2School of Pharmacy, RK University, Rajkot, Gujarat, India
*Corresponding Author: Jignasha Thumar, Department of Microbiology and Biotechnology, Gujarat University, Ahmedabad, Gujarat, India.
January 17, 2023; Published: February 14, 2023
In the current investigation, our primary focus is on the synthesis of extracellular protease, and we aim to identify the factors that produce the most favorable results. Bor S17B13 is a halo-tolerant, gram +ve, bacillus sp. isolated from soil associated mangrove vegetation in the Indian state of Maharashtra. Based on the sequencing of the 16s rRNA gene, it was discovered that strain Bor S17B13 had a connection to the Priestia aryabhattai strain. It is interesting to note that Bor S17B13 reveals a biphasic growth pattern despite having two different sources of nitrogen and just one source of carbon. During the second log phase, which was also the time in which lavish growth was noted and the maximum level of protease production was recorded at the same time, The growth rate achieved with an inoculum concentration of 9% v/v was optimal. Optimization for the isolate revealed that it can grow on 0-20% NaCl concentrations and even produce the protease enzyme, confirming the Bor S17B13 isolate's halotolerant nature. Maximum production of protease was at 0% NaCl (w/v) (171 U/ml), and optimum growth was also seen at the same concentration. Growth and protease activity were greatest at pH 7 (172 U/ml), but they can grow in the pH range of 7 to 9. When up-scaling a product to an industrial level, the use of a specialised medium for a possible isolate plays a very important and critical function. This helps to avoid economic losses and significantly lowers the manufacturing costs of the enzyme product.
Keywords: Mangrove Ecosystem; Halotolerant; Protease; NaCl; Growth pH; Optimization
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