Acta Scientific Pharmaceutical Sciences (ASPS)(ISSN: 2581-5423)

Research Article Volume 7 Issue 3

Marine-derived Producer Bor S17B13 and its Response to Variations in Salt (NaCl) Concentration and pH in the Growth Medium

Priyanka Sawant1, Kartik Vikani2 and Jignasha Thumar1*

1Department of Microbiology and Biotechnology, Gujarat University, Ahmedabad, Gujarat, India
2School of Pharmacy, RK University, Rajkot, Gujarat, India

*Corresponding Author: Jignasha Thumar, Department of Microbiology and Biotechnology, Gujarat University, Ahmedabad, Gujarat, India.

Received: January 17, 2023; Published: February 14, 2023


In the current investigation, our primary focus is on the synthesis of extracellular protease, and we aim to identify the factors that produce the most favorable results. Bor S17B13 is a halo-tolerant, gram +ve, bacillus sp. isolated from soil associated mangrove vegetation in the Indian state of Maharashtra. Based on the sequencing of the 16s rRNA gene, it was discovered that strain Bor S17B13 had a connection to the Priestia aryabhattai strain. It is interesting to note that Bor S17B13 reveals a biphasic growth pattern despite having two different sources of nitrogen and just one source of carbon. During the second log phase, which was also the time in which lavish growth was noted and the maximum level of protease production was recorded at the same time, The growth rate achieved with an inoculum concentration of 9% v/v was optimal. Optimization for the isolate revealed that it can grow on 0-20% NaCl concentrations and even produce the protease enzyme, confirming the Bor S17B13 isolate's halotolerant nature. Maximum production of protease was at 0% NaCl (w/v) (171 U/ml), and optimum growth was also seen at the same concentration. Growth and protease activity were greatest at pH 7 (172 U/ml), but they can grow in the pH range of 7 to 9. When up-scaling a product to an industrial level, the use of a specialised medium for a possible isolate plays a very important and critical function. This helps to avoid economic losses and significantly lowers the manufacturing costs of the enzyme product.

 Keywords: Mangrove Ecosystem; Halotolerant; Protease; NaCl; Growth pH; Optimization


  1. I Younes and M Rinaudo. “Chitin and chitosan preparation from marine sources. Structure, properties and applications.”. Marine Drugs3 (2015): 1133-1174.
  2. P Palsaniya., et al. “Optimization of alkaline protease production from bacteria isolated from soil”. Journal of Microbiology and Biotechnology6 (2012): 858-865.
  3. Q K Beg., et al. “De-repression and subsequent induction of protease synthesis by Bacillus mojavensis under fed-batch operations”. Process Biochemistry10 (2002): 1103-1109.
  4. P Sawant and J Thumar. “Isolation of Marine Microbugs from Mangrove Ecosystem and Screening of Industrially Important Enzymes”. Acta Scientific Microbiology (2022): 35-39.
  5. R K Patel., et al. “Production of Extracellular Halo-alkaline Protease from a Newly Isolated Haloalkaliphilic Bacillus Isolated from Seawater in Western India”. World Journal of Microbiology and Biotechnology 22.4 (2006): 375-382.
  6. “Microbial Enzymes and Biotechnology” (1990).
  7. R C Kasana., et al. “A rapid and easy method for the detection of microbial cellulases on agar plates using Gram’s iodine”. Current Microbiology5 (2008): 503-507.
  8. R Zalak., et al. “Isolation and Screening of Multivariant Enzyme Producing Endophytic Bacteria from Citrus limon”. Acta Scientific Microbiology 5 (2022): 2581-3226.
  9. Hagihara, “The Enzymes”. 2nd edn. 4. Boyer. P. Acad. Press New York, (1958).
  10. J T Thumar and S P Singh. “Secretion of an alkaline protease from a salt- tolerant and alkaliphilic, Streptomyces clavuligerus strain Mit-1”. Brazilian Journal of Microbiology4 (2007): 766-772.
  11. J Mamangkey., et al. “Isolation and enzyme bioprospection of bacteria associated to Bruguiera cylindrica, a mangrove plant of North Sumatra, Indonesia”. Biotechnology Reports 30 (2021): e00617.
  12. C Suganthi., et al. “Screening and optimization of protease production from a halotolerant Bacillus licheniformis isolated from saltern sediments”. Journal of Genetic Engineering and Biotechnology 1 (2013): 47-52.
  13. A S S Ibrahim., et al. “Production of extracellular alkaline protease by new halotolerant alkaliphilic Bacillus NPST-AK15 isolated from hyper saline soda lakes”. Electronic Journal of Biotechnology 18.3 (2015): 236-243.
  14. Ö Ateş., et al. “Isolation and identification of alkaline protease producer halotolerant Bacillus licheniformis strain BA17”. Annals of Microbiology3 (2007): 369.
  15. P Sridhara., et al. “Isolation, Characterization, and Optimization of Protease-Producing Bacterium Bacillus thuringiensis from Paddy Field Soil”. (2021).
  16. M M S Asker., et al. “Purification and characterization of two thermostable protease fractions from Bacillus megaterium”. Journal of Genetic Engineering and Biotechnology 2 (2013): 103-109.
  17. N Abdullah Al-Dhabi., et al. “Characterization and fermentation optimization of novel thermo stable alkaline protease from Streptomyces sp. Al-Dhabi-82 from the Saudi Arabian environment for eco-friendly and industrial applications”. Journal of King Saud University – Science 1 (2020): 1258-1264.


Citation: Jignasha Thumar., et al. “Marine-derived Producer Bor S17B13 and its Response to Variations in Salt (NaCl) Concentration and pH in the Growth Medium". Acta Scientific Pharmaceutical Sciences 7.3 (2023): 04-10.


Copyright: © 2022 Jignasha Thumar., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


Acceptance rate32%
Acceptance to publication20-30 days

Indexed In

News and Events

  • Certification for Review
    Acta Scientific certifies the Editors/reviewers for their review done towards the assigned articles of the respective journals.
  • Submission Timeline for Upcoming Issue
    The last date for submission of articles for regular Issues is July 30, 2024.
  • Publication Certificate
    Authors will be issued a "Publication Certificate" as a mark of appreciation for publishing their work.
  • Best Article of the Issue
    The Editors will elect one Best Article after each issue release. The authors of this article will be provided with a certificate of "Best Article of the Issue"
  • Welcoming Article Submission
    Acta Scientific delightfully welcomes active researchers for submission of articles towards the upcoming issue of respective journals.

Contact US