Acta Scientific Pharmaceutical Sciences (ASPS)(ISSN: 2581-5423)

Research Article Volume 7 Issue 2

Preparation and Evaluation of Phytophospholipid Complex of Phenolic Fractions of G. glabra for Antioxidant and Antimicrobial Activity

Jyotsna Anandrao Saonere*, Madhuri A Channawar, Nitin I Kochar, Deepak Mohale and Anil V Chandewar

Pataldhamal Wadhwani College of Pharmacy, Yavatmal (M.S), India

*Corresponding Author: Jyotsna Anandrao Saonere, Pataldhamal Wadhwani College of Pharmacy, Yavatmal (M.S), India.

Received: December 27, 2022; Published: January 11, 2023

Abstract

Objectives: The objective of this study was to evaluate an antioxidant and antimicrobial effect of microencapsulated phyto-phospholipid complex of polyphenolic fractions of Glycerrhiza glabra.

Methods: Phenolic fractions of Glycerrhiza glabra were incorporated in microencapsulated Phytophospholipid complex. Phytophospholipid complex of phenolic fractions were prepared by rotary evaporation method. Total Phenolic content (TPC) was determined by folin cio-caltechu method. In-vitro antioxidant activity of phenolic fractions and phytophospholipid complex of phenolic fractions were determined by DPPH method. Antimicrobial potential of fractions as well as phytophospholipid complex of phenolic fractions were evaluated by agar well method on different seven species of bacteria and fungi.

Results: Total five formulation P1, P2, P3, P4 and P5 of phytophaspholipid complex loaded with phenolic fraction were prepared by rotary evaporation method Amongst five formulation, P2 formulation showed maximum Entrapment efficiency (EE), particle size, zeta potential, drug content and drug release. Antioxidant study revealed that prepared phytophaspholipid complex showed optimum free radical scavenging effect. Antimicrobial study showed that ethyl acetate fraction of G. glabra showed zone of inhibition against all the selected microorganism except P. vulgaris, Optimized microencapsulated complex P2 formulation showed zone of inhibition against all the species except P. vulgaris.

Conclusion: Study revealed that amongst five formulation P2 phytophospholipid complex showed better skin permeation, drug content, sustain release effect, with optimum free radical scavenging effect, and excellent antimicrobial effect than the phenolic fraction.

Keywords: Polyphenols; Free Radical Scavenging Effect; Phytophaspholipid Complex; Antimicrobial Effect

References

  1. Ayoub Z., et al. “Medicinal plants as potential source of antioxidant agents review”. Asian Journal of Pharmaceutical and Clinical Research 6 (2018): 50-56.
  2. Haworth JE. “Natural Antioxidants Review”. In: Proceedings of the 56th Reciprocal Meats Conference (2003): 95-98.
  3. Saharan VA., et al. “Process optimisation, characterization and evaluation of resveratrol-phospholipid complexes using box-behnken statistical design”. International Current Pharmaceutical Journal 3 (2014;): 301-308.
  4. Tan Q., et al. “Design and evaluation of a novel evodiamine-phospholipid complex for improved oral bioavailability”. AAPS PharmSciTech (2012).
  5. Pande SD., et al. “Preparation and evaluation of phytosomes of Pomegrane peels”. International Journal of PharmTech Research 4 (2014): 416-422.
  6. Sikwarwar MS., et al. “Preparation, characterization and evaluation of marsupsin-phospholipid complex”. AAPS PharmSciTech 9 (2008): 129-137.
  7. Mishra GP., et al. “Preparation and evaluation of phytosomes of Vitex negundolinn”. Novel Science International Journal of Pharmaceutical Science 10 ( 2012): 671-673.
  8. Karimi N., et al. “Phytosome and Liposome: The beneficial encapsulation systems in drug delivery and food application”. Applied Food Biotechnology3 (2015): 17-27.
  9. Jain A., et al. “Sida cardifolia: A review”. Journal of Applied Pharmaceutical Science02 (2011): 23-31.
  10. Sanchez CM., et al. “Review: Methods used to evaluate the free radical scavenging activity in foods and biological systems”. Journal of Food Science and Technology 8 (2002): 121-137.
  11. Song Y., et al. “Preparation and properties of a Silybin-phospholipid complex”. Pharmazie 63 (2008): 35-42.
  12. Tian M., et al. “Extraction of Glycyrrhizic Acid and Glabridin from Licorice”. International Journal of Molecular Sciences4 ( 2008): 571-577.
  13. Momin MA., et al. “Phytopharmacological evaluation of ethanol extract of Sida cordifolia Roots”. Asian Pacific Journal of Tropical Biomedicine 4.1 ( 2014): 18-24.
  14. Lin JY., et al. “Determination of total phenolic and flavonoidcontents in selected fruits and vegetables, as well as their stimulatory effects on mouse splenocyte proliferation”. Food Chemistry 101 (2007): 140-147.
  15. Alhakmani F., et al. “Estimation of total phenoliccontent, in-vitro antioxidant and anti-inflammatory activity of flowers of Moringa oleifera”. Asian Pacific Journal of Tropical Biomedicine 3 (2013): 623-627.
  16. Ahmad A., et al. “Physicochemical and phytochemical standardization with HPTLC fingerprinting of Nigella sativa Seeds”. Pakistan Journal of Pharmaceutical Sciences 27 ( 2014): 1175-1182.
  17. Brand-Williams W., et al. “Use of a free radical method to evaluate antioxidant activity”. Lebenson Wiss Technology 28 (1995): 25-30.
  18. Mensor LL,. et al. “Screening of Brazilian plant extracts for antioxidant activity by the use of DPPH free radical method”. Phytotherapy Research 15 (2001): 127-130.
  19. Mei Lu., et al. “Phyto-phospholipid complexes (phytosomes): A novel strategy to improve the bioavailability of active constituents”. Asian Journal of Pharmaceutical Sciences3 (2019): 265-274.
  20. Ashwini SD., et al. “Preparation and evaluation of phytosomes containing methanolic extract of leaves of Aegle Marmelos (Bael)”. International Journal of PharmTech Research 6 (2015): 231-240.
  21. Rathnam G., et al. “Preparation andevaluation of carbopol based nasal gels for systemic delivery of progesterone”. International Journal of Pharma. Research and Development1 (2005): 1-11.
  22. Bruno and Bombardelli Complexes of flavolignans with Phospholipids preparation there of and associated pharmaceutical compositions United state patent US4764508A, (1985).
  23. , et al. “Boswellic acid compositions and preparation” there of United state patent US 5629351 A (1997).
  24. Kessler M., et al. “Anti- and pro-oxidant activity of rutin and quercetin derivatives”. Journal of Pharmacy and Pharmacology 55 (2003): 131-142.
  25. Soong Y., et al. “Antioxidant activity and phenolic content of selected fruit seeds”. Food Chemistry 88 (1997): 411-418.
  26. Suzilla WY., et al. “Formulation and evaluation of antimicrobial herbosomal gel from Quercus infectoria extract”. IOP Conf. Ser.: Mater. Sci. Eng. 736 (2020): 022030.
  27. Korhalkar A., et al. International Journal of Current Microbiology and Applied Sciences 1 (2014): 329-336.

Citation

Citation: Jyotsna Anandrao Saonere., et al. “Preparation and Evaluation of Phytophospholipid Complex of Phenolic Fractions of G. glabra for Antioxidant and Antimicrobial Activity". Acta Scientific Pharmaceutical Sciences 7.2 (2023): 37-44.

Copyright

Copyright: © 2022 Jyotsna Anandrao Saonere., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.




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