Preparation and Evaluation of Phytophospholipid Complex of Phenolic Fractions of G. glabra for Antioxidant and Antimicrobial Activity
Jyotsna Anandrao Saonere*, Madhuri A Channawar, Nitin I Kochar, Deepak Mohale and Anil V Chandewar
Pataldhamal Wadhwani College of Pharmacy, Yavatmal (M.S), India
*Corresponding Author: Jyotsna Anandrao Saonere, Pataldhamal Wadhwani College of Pharmacy, Yavatmal (M.S), India.
December 27, 2022; Published: January 11, 2023
Objectives: The objective of this study was to evaluate an antioxidant and antimicrobial effect of microencapsulated phyto-phospholipid complex of polyphenolic fractions of Glycerrhiza glabra.
Methods: Phenolic fractions of Glycerrhiza glabra were incorporated in microencapsulated Phytophospholipid complex. Phytophospholipid complex of phenolic fractions were prepared by rotary evaporation method. Total Phenolic content (TPC) was determined by folin cio-caltechu method. In-vitro antioxidant activity of phenolic fractions and phytophospholipid complex of phenolic fractions were determined by DPPH method. Antimicrobial potential of fractions as well as phytophospholipid complex of phenolic fractions were evaluated by agar well method on different seven species of bacteria and fungi.
Results: Total five formulation P1, P2, P3, P4 and P5 of phytophaspholipid complex loaded with phenolic fraction were prepared by rotary evaporation method Amongst five formulation, P2 formulation showed maximum Entrapment efficiency (EE), particle size, zeta potential, drug content and drug release. Antioxidant study revealed that prepared phytophaspholipid complex showed optimum free radical scavenging effect. Antimicrobial study showed that ethyl acetate fraction of G. glabra showed zone of inhibition against all the selected microorganism except P. vulgaris, Optimized microencapsulated complex P2 formulation showed zone of inhibition against all the species except P. vulgaris.
Conclusion: Study revealed that amongst five formulation P2 phytophospholipid complex showed better skin permeation, drug content, sustain release effect, with optimum free radical scavenging effect, and excellent antimicrobial effect than the phenolic fraction.
Keywords: Polyphenols; Free Radical Scavenging Effect; Phytophaspholipid Complex; Antimicrobial Effect
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