Effect of Active Fraction Isolated from the Leaf Extract of Allmania nodiflora on Plasma Glucose Concentration and Lipid Profile in Streptozotocin-Induced Diabetic Rats
Anandteerth Deshpande*, Venkatesan Natarajan and Boguda Anantharamaiah Vishwanath
Aditya Bangalore Institute of Pharmacy Education and Research, Bangalore, Karnataka, India
*Corresponding Author: Anandteerth Deshpande, Aditya Bangalore Institute of Pharmacy Education and Research, Bangalore, Karnataka, India.
September 21, 2022; Published: December 14, 2022
The current study's objective was to determine how active fractions from Allmania nodiflora leaves affected the lipid and serum glucose profiles in normal and diabetic rats. Streptozotocin (45 mg/kg) activated diabetes in wistar rats. Ethanolic extract of Allmania nodiflora (EEAN) leaf was given orally at doses of 200 and 400 mg/kg.p.o. Metformin (50 mg/kg p.o.) was administered as the usual anti-diabetic medication. When compared to normal control rats, EEAN considerably (p < 0.05) decreased the fasting blood glucose (FBG) level. In addition, column chromatography was used to isolate an active compound, which was given the innocuous name AN-1. A study of the hypoglycemic and hypolipidemic effects of AN-1 (100 mg/kg) was conducted. In diabetic rats, AN-1 significantly (p < 0.05) decreased FBG levels while simultaneously lowering cholesterol and triglycerides and raising HDL levels. This finding suggests that AN-1 has demonstrated anti-diabetic and anti-hyperlipidemic activity, and it offers a rational scientific justification for the use of anti-diabetic medications.
Keywords: Blood Glucose; Allmania nodiflora; Lipid Profile; GC-MS
- Kahn CR and Shechter Y. “Insulin oral hypoglycemic agents and the pharmacology of the endocrine pancreas in the pharmacological basis of therapeutics”. Edited by Gillman AG, Rail TW, Nies AS, Taylor, Pergamon press, New York, (1991): 1463-1495.
- Aikinson MA and Maclaren NK. “The pathogenesis of insulin dependent diabetes”. The New England Journal of Medicine 331 (1994): 1428-1436.
- Takeshi K., et al. “Report of the committee on the classification and diagnostic criteria of diabetes mellitus”. Diabetes Research and Clinical Practice 55 (2002): 65-85.
- King H., et al. “Global burden of diabetes 1995-2025: prevalence, numerical estimates, and projection”. Diabetes Care 21 (1998): 1414-1431.
- De-Fronzo RA., et al. “Pathogenesis of NIDDM”. International Text book of Diabetes mellitus, 2nd edition, Chichester, John Wiley, England (1997): 635-712.
- Donath MY and Ehses JA. “Type I and type II diabetes: NOD the diabetes we thought it was”. Proceedings of the National Academy of Sciences 103 (2006): 12217-12218.
- Hansotia T and Drucker DJ. “GIP and GLP-1 as incretin hormones: lessons from single and double incretin receptor knockout mice”. Regulatory Peptides 128 (2005): 125-134.
- Pepato MT., et al. “Fruit of the jambolan tree (Eugenia jambolana) and experimental diabetes”. Journal of Ethnopharmacology 96 (2005): 43-48.
- Grover JK and Yadav SP. “Pharmacological actions and potential uses of Momordicacharantia: A review”. Journal of Ethnopharmacology 93 (2004): 123-132.
- Jain AK., et al. “Folklore claims on some medicinal plants used by Bheel tribe”. Indian Journal of Traditional Knowledge1 (2010): 105-107.
- Kavishankar N., et al. “Diabetes and medicinal plants-A review”. International Journal Of Pharmaceutical And Bio-Medical Science 3 (2011): 65-80.
- Ogbeide M Parvez. “Identification of the flavonoids in papilionaceae flowers using paper chromatography”. Journal of Liquid Chromatography 15 (1992): 2989-2996.
- Sreenivasan KK and S Sankarasubramanian. “Chemical investigation of Allmania nodiflora”. Journal of Health Sciences (1984): 156-158.
- Chio LC and KF Huang. “Studies on the constituents of Allmania nodiflora (L.)”. DC. Master thesis, Providence University, Taiwan (1995): 1-14.
- Sriplang K., et al. “Effects of Orthosiphon stamineus aqueous extract on plasma glucose concentration and lipid profile in normal and streptozotocin-induced diabetic rats”. Journal of Ethnopharmacology 109 (2007): 510-514.
- Venkatesan N and Anton Smith A. “Effect of active fraction isolated from the leaf extract of Dregea volubilis [Linn.] on plasma glucose concentration and lipid profile in streptozotocin-induced diabetic rats”. Springer Plus 2 (2013): 394.
- Masana L., et al. “Substituting non-HDL cholesterolwith LDL as a guide for lipid-lowering therapy increases the number of patients with indication for therapy”. Atherosclerosis 2 (2012): 471-475.
- Heber MF., et al. “Prenatal hyperandrogenism and lipid profile during different age stages: an experimental study”. Fertility and Sterility2 (2013): 551-557.
- Tripathi J., et al. “Anti-diabetic activity of Diplocyclo spalmatus in Streptozotocin-Induced Diabetic Mice”. Indian Journal of Pharmaceutical Education and Research 46.4 (2012): 352-359.
- Cao J., et al. “Antidiabetic effect of burdock (Arctium lappa) root ethanolic extract on streptozotocin-induced diabetic rats”. African Journal of Biotechnology 11.37 (2012): 9079-9085.
- Henry N Ginsberg., et al. “Regulation of Plasma Triglycerides in Insulin Resistance and Diabetes”. Archives of Medical Research 36 (2005): 232-240.
- Venkatesan N and Anton Smith A. “Effect of active fraction isolated from the leaf extract of Leptadenia reticulata on plasma glucose concentration and lipid profile in streptozotocin-induced diabetic rats”. Chinese Journal of Natural Medicines5 (2014): 463-468.