Acta Scientific Pharmaceutical Sciences (ASPS)(ISSN: 2581-5423)

Research Article Volume 7 Issue 1

Evaluation of the Antimicrobial Activity of the Punica granatum Peel Extract Against Isolated MDR salmonella typhimurium

Rahul Prabhu Gaikwad*, Madhuri A Channawar, Deepak Mohale, Nitin I Kochar and Anil V Chandewar

Pataldhamal Wadhwani College of Pharmacy, Yavatmal (M.S), India

*Corresponding Author: Rahul Prabhu Gaikwad, Pataldhamal Wadhwani College of Pharmacy, Yavatmal (M.S), India.

Received: October 27, 2022 ; Published: December 07, 2022

Abstract

Objectives: To produce broad-spectrum antimicrobial compounds, it is still important to assess the antibacterial potential of medicinal plants. Traditional medicine is one of the most generally available types of treatment in disadvantaged countries. The current study's goal was to assess the antibacterial activity of Punica granatum peel extract against MDR salmonella typhimurium.

Methods: This study tested Punica granatum's antibacterial efficacy using the disk diffusion method. Amoxicillin and Punica granatum peel extract are effective against MDR Salmonella typhimurium.

Results: It was discovered that Punica granatum crude extract worked well against MDR salmonella typhimurium. The minimum inhibitory concentration (MIC) and zone of inhibition values for the crude Punica granatum Peel Extract were found to be 1 mg/ml and 09 ± 0.50 mm to 19.50 ± 0.00 mm, respectively.

Conclusions: According to the study's findings, Punica granatum crude Peel Extract has in-vitro antibacterial activity, providing early evidence that the plant may be used to treat MDR infections.

Keywords: Salmonella Typhimurium; Multidrug Resistance; Punica granatum; Zones of Inhibition; Minimum Inhibitory Concentration (MIC)

References

  1. Anchez-Lamar AS., et al. “Assessment of the genotoxic risk of Punica granatum (Punicaceae) whole fruit extracts”. Journal of Ethnopharmacology 115.3 (2007): 416-422.
  2. Aqil F., et al. “Effect of certain bioactive plant extracts on clinical isolates of beta-lactamase producing methicillin resistant Staphylococcus aureus”. Journal of Basic Microbiology 45 (2005): 106-114.
  3. Aviram M., et al. “Pomegranatem juice consumption for 3 years by patients with carotid artery stenosis reduces common carotid intima-media thickness, blood pressure and LDL oxidation”. Clinical Nutrition 23 (2004): 423-433.
  4. Aviram M., et al. “Pomegranate phenolics from the peels, arils, and flowers are antiathero- genic: studies in vivo in atherosclerotic apolipoprotein e- deficient (E0) mice and in vitro in cultured macrophages and lipoproteins”. Journal of Agricultural and Food Chemistry 56 (2008): 1148-1157.
  5. Barmet H. “The natural pharmacy: An encyclopedic illustrated guide to medicine from nature”. Mirriampolunin and Christopher Robins, Great Britain, (1992).
  6. Boakye Y., et al. “In vitro and in vivo antioxidant properties of Phyllanthus muellerianus and its major constituent, geraniin”. Oxidants and Antioxidants in Medical Science 5 (2016): 70.
  7. Braga L C., et al. “Pomegranate extract inhibits Staphylococcus aureus growth and subsequent enterotoxin production”. Journal of Ethnopharmacology1-2 (2005): 335-339.
  8. Choi Jang-gi., et al. “In Vitro and In Vivo Antibacterial Activity of Punica granatum Peel Ethanol Extract against Salmonella”. Evidence-Based Complementary and Alternative Medicine: eCAM (2011). 690518. 10.1093/ecam/nep105.
  9. Clinical and Laboratory Standards Institute. “Performance Standards for Antimicrobial Disk Susceptibility Tests. Approved Standards”. CLSI document M2-A7. Wayne, Pa, USA, (2001).
  10. GBD 2017 Causes of Death Collaborators. “Global, regional, and national age-sex-specific mortality for 282 causes of death in 195 countries and territories, 1980-2017: a systematic analysis for the Global Burden of Disease Study 2017”. Lancet 392 (2018): 1736-1788.
  11. Gebreyes W A., et al. “Trends in antimicrobial resistance, phage types and integrons among Salmonella serotypes from pigs, 1997-2000”. Journal of Antimicrobial Chemotherapy 53 (2004): 997-1003.
  12. Kwak HM., et al. “Quantitative analysis of antioxdants in Korea pomegranate Husk (Granati pericarpium) cultivated in different site”. Journal of the Korean Society for Applied Biological Chemistry 48 (2005): 431-434.
  13. Lansky E P and R A Newman. “Punica granatum (pomegranate) and its potential for prevention and treatment of inflammation and cancer”. Journal of Ethnopharmacology 2 (2007): 177-206.
  14. Machado TDB., et al. “Antimicrobial ellagitannin of Punica granatum fruits”. Journal of the Brazilian Chemical Society5 (2002): 606-610.
  15. National Center for Biotechnology Information. PubChem Compound Summary for CID 16129869, Punicalagin (2022).
  16. National Center for Biotechnology Information. PubChem Compound Summary for CID 370, Gallic acid (2022).
  17. National Center for Biotechnology Information (2022). PubChem Compound Summary for CID 5281855, Ellagic acid (2022).
  18. Naz S., et al. “Antibacterial activity directed isolation of compounds from Punica granatum”. Journal of Food Science9 (2007): M341-M345.
  19. Nostro A., et al. “Effects of combining extracts (from propolis or Zingiber officinale) with clarithromycin on Helicobacter pylori”. Phytotherapy Research3 (2006): 187-190.
  20. Ochwang'I D O., et al. “Cytotoxic activity of medicinal plants of the Kakamega County (Kenya) against drug-sensitive and multidrug-resistant cancer cells”. Journal of Ethnopharmacology 215 (2018): 233-240.
  21. Okunji C O., et al. “An antifungal spirostanol saponin from fruit pulp of Dracaena mannii”. International Journal of Crude Drug Research3 (1990): 193-199.
  22. Parmar H S and A Kar. “Protective role of Citrus sinensis, Musa paradisiaca, and Punica granatum peels against diet- induced atherosclerosis and thyroid dysfunctions in rats”. Nutrition Research11 (2007): 710-718.
  23. Qian H., et al. “Discovery of seven novel mutations of gyrB, parC and parE in Salmonella Typhi and Paratyphi strains from Jiangsu Province of China”. Scientific Report 10 (2020):
  24. Ricci D., et al. “Antioxidant activity of Punica granatum fruits”. Fitoterapia4 (2006): 310-312.
  25. Singh R P., et al. “Studies on the antioxidant activity of pomegranate (Punica granatum) peel and seed extracts using in vitro models”. Journal of Agricultural and Food Chemistry1 (2002): 81-86.
  26. Taguri T., et al. “Antimicrobial activity of 10 different plant polyphenols against bacteria causing food- borne disease”. Biological and Pharmaceutical Bulletin12 (2004): 1965-1969.
  27. Threlfall EJ., et al. “Spread of resistance from food animals to man-the UK experience”. Acta Veterinaria Scandinavica. Supplementum 93 (2000): 63-68.
  28. Tshibangu JN., et al. “Screening of African medicinal plants for antimicrobial and enzyme inhibitory activity”. Journal of Ethnopharmacology 80 (2002): 25-35.
  29. Uddin MS., et al. “Ashraf Nootropic and anti-Alzheimer's actions of medicinal plants: molecular insight into therapeutic potential to alleviate Alzheimer's neuropathology”. Molecular Neurobiology 56 (2019): 4925-4944.
  30. Ugboko H and De N. “Mechanisms of Antibiotic resistance in Salmonella Typhi”. International Journal of Current Microbiology and Applied Sciences 3 (2014): 461-476.
  31. Voravuthikunchai S P., et al. “Inhibitory effects of active compounds from Punica granatum pericarp on verocytotoxin production by enterohemorrhagic Escherichia coli O157:H7”. Journal of Health Science5 (2005): 590-596.
  32. Zhang J., et al. “Antiviral activity of tannin from the pericarp of Punica granatum against genital Herpes virus in vitro”. Zhongguo Zhong yao za zhi 20.9 (1995): 556-576.

Citation

Citation: ARahul Prabhu Gaikwad., et al. “Evaluation of the Antimicrobial Activity of the Punica granatum Peel Extract Against Isolated MDR salmonella typhimurium". Acta Scientific Pharmaceutical Sciences 7.1 (2023): 02-07.

Copyright

Copyright: © 2022 Rahul Prabhu Gaikwad., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.




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