Acta Scientific Pharmaceutical Sciences (ASPS)(ISSN: 2581-5423)

Review Article Volume 6 Issue 9

Thin Layer Chromatography (TLC) VS. Paper Chromatography: A Review

Shashank Tiwari1* and Shreya Talreja2

1Professor, Director (Academic and Research), Lucknow Model College of Pharmacy, Lucknow, India
2M.Pharm, Goel Institute of Pharmacy and Sciences, Lucknow, India

*Corresponding Author: Shashank Tiwari, Professor, Director (Academic and Research), Lucknow Model College of Pharmacy, Lucknow, India.

Received: July 08, 2022; Published: August 17, 2022


In the field of analytical chemistry and various pharmaceutical fields the separation techniques are widely used to study specific compounds from a mixture or a complex material. One such separation technique is called Chromatography. It takes place on the basis of the relative volume of each solute present in the moving fluid stream. The discovery of chromatography was done by Mikhail S. Tsvet, who was a Russian botanist in the year 1901. The separation is done through stationary phase and a mobile phase. Chromatography is used for separation, analysis, and purification of various components which can include food, pesticides, pharmaceuticals, tissue extracts etc. In this paper 2 major types of chromatography are discussed; these include Paper Chromatography and Thin Layer Chromatography. The principles of both the types will be discussed and the contrast will be established between the two in a tabular format.

Keywords: Analytic; Pharmaceutical; Chromatography; Mobile Phase; Stationary Phase


  1. Bele AA and Khale A. “An overview on thin layer chromatography”. International Journal of Pharmaceutical Sciences and Research2 (2011): 256.
  2. Dr D. “What are the advantages of thin layer chromatography over paper chromatography?” Auriga Research Private limited. (20115): 1-10.
  3. Gupta M., et al. “Paper Chromatography: A Review”. JETIR 10 (2018): 2349-5162
  4. G Zweig and J Sherma. “Paper Chromatography—Past, Present and Future”. Journal of Chromatographic Science 6 (1973): 279-283.
  5. MG Rasul. “Extraction, Isolation and Characterization of Natural Products from Medicinal Plants”. International Journal of Basic Sciences and Applied Computing6 (2011): 1-6.
  6. PW Smit., et al. “An overview of the clinical use of filter paper in the diagnosis of tropical diseases”. The American Journal of Tropical Medicine and Hygiene2 (2014): 195-210.
  7. Coskun, “Separation techniques: chromatography”. Northern Clinics of Istanbul 3.2 (2016): 156.
  8. Singhal S., et al. “Pharmaceutical Analysis II”. Thin layer chromatography, Pragati prakashan, First edition (2009): 98-111.
  9. Vidya Sagar. “Instrumental methods of drug analysis”. Pharma Med Press, First edition (2009):
  10. W Wolfson., et al. “An improved apparatus and procedure for ascending paper chromatography on large size filter paper sheets”. Science2839 (1949): 541-543.


Citation: Shashank Tiwari and Shreya Talreja. “Thin Layer Chromatography (TLC) VS. Paper Chromatography: A Review". Acta Scientific Pharmaceutical Sciences 6.9 (2022): 05-09.


Copyright: © 2022 Shashank Tiwari and Shreya Talreja. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


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