Dasari Vasavi Devi* and Shaik Umme Salma
Pharmaceutical Analysis, P. Ramireddy Memorial College of Pharmacy, India
*Corresponding Author: Dasari Vasavi Devi, Pharmaceutical Analysis, P. Ramireddy Memorial College of Pharmacy, India.
Received: January 06, 2022; Published: March 02, 2022
High performance liquid chromatography is at present one of the most sophisticated tool of the analysis. The assessment of Ceritinib and Lenvatinib was run by RP-HPLC. The Methanol: Phosphate buffer in the ratio of 70: 30% v/v which consists of buffer pH 3.0 was used as a mobile phase. The stationary phase used in the present analysis was Inertsil ODS column (4.6 x 150 mm, 5 µm). The PDA detector with detecting wavelength of 260 nm was used for identification of the separated drugs. The constant flow rate with 1ml/min was employed for efficient separation of the components. The linearity range of Ceritinib and Lenvatinib was found to be from 100 - 500 ppm of Ceritinib and 1 - 5 ppm of Lenvatinib. Linear regression coefficient was not more than 0.999. The values of% RSD are less than 1% indicating precision of the method. The percentage recovery differs from 98 - 102% of Ceritinib and lenvatinib. It surmised that the method was viewed as simple, linear, precise and accurate as per ICH requirements. The method was viewed as having reasonable application in routine lab analysis with high level of accuracy and precision.
Keywords: Inertsil ODS; Ceritinib and Lenvatinib; RP-HPLC
Citation: Dasari Vasavi Devi and Shaik Umme Salma. “Simultaneous Estimation of Ceritinib and Lenvatinib Using RP-HPLC Method". Acta Scientific Pharmaceutical Sciences 6.3 (2022): 02-07.
Copyright: © 2022 Dasari Vasavi Devi and Shaik Umme Salma. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.