Acta Scientific Pharmaceutical Sciences (ASPS)(ISSN: 2581-5423)

Research Article Volume 6 Issue 3

Protein Crosslinking Onto Gold Nanoparticles by the Use of Gamma Radiation

Janaína A G Barros1*, Jorge G S Batista1, Vitor de Moraes Zamarion2 and Ademar B Lugão1

1Instituto de Pesquisas Energéticas e Nucleares (IPEN-CNEN/SP) - Avenida Lineu Prestes, São Paulo, SP, Brazil
2Fundamental Chemistry Department, University of São Paulo (IQ-USP) Avenida Lineu Prestes, São Paulo, SP, Brazil

*Corresponding Author: Janaína A G Barros, Instituto de Pesquisas Energéticas e Nucleares (IPEN-CNEN/SP) - Avenida Lineu Prestes, São Paulo, SP, Brazil.

Received: November 30, 2021; Published: February 21, 2022

Abstract

Gold nanoparticles (AuNP) are being investigated for diagnostic and therapeutic nanomedicines considering their low toxicity and stability against oxidation, among other features. AuNP may be administered site-specifically or intravenously for diagnostic imaging by computed tomography or for therapy. Albumin functionalization of AuNP whether linked by chemical or physical processes may provide unique features to the system regarding biopharmaceutical aspects. Recently albumin crosslinking has been studied by radiation induced methods. This work aimed the development of radiation crosslinked bovine serum albumin (BSA) capped AuNP for improved administration of the nanoparticles. For such purpose AuNP were synthesized using 2.5 mM sodium tetrachloroaurate (III) dehydrate and 1.67 mM resveratrol in water. The mixture was allowed to stand for 12 hours. AuNP capping was performed by BSA addition followed by slow addition of ice-cold ethanol to reach 5 mg mL-1 BSA in a 30% (v/v) ethanol solution. The mixture was allowed to rest overnight at ± 4 oC. BSA crosslinking onto AuNP was performed by gamma irradiation at doses of 2.5, 5, 7.5, 10 and 15 kGy. AuNP were synthesized with approximately 38 nm, whereas BSA Capped AuNP presented size ranging from 70 nm up to 200 nm, as revealed by dynamic light scattering. AuNP and BSA capped AuNP morphology was evaluated by transmission electron microscopy using negative staining and revealed protein aggregation onto AuNPs and its surroundings. Irradiation led to no shifts in maximum absorbance for both nanoparticles. Protein crosslinking was confirmed by bityrosine and increased with dose.

Keywords: Gold Nanoparticles; Bovine Serum Albumin; Resveratrol; Green Synthesis; BSA Capping; Functionalized Nanoparticles; Protein Crosslinking; Gamma Radiation

References

  1. Reddy GAK., et al. "Nano silver - a review”. International Journal of Advances in Pharmaceutical Sciences 1 (2012): 09.
  2. Makarov VV., et al. ""Green” Nanotechnologies: Synthesis of Metal Nanoparticles Using Plants”. Acta Naturae1 (2014): 35-44.
  3. Shukla r., et al. "Soybeans as a Phytochemical Reservoir for the Production and Stabilization of Biocompatible Gold Nanoparticles”. Small9 (2008): 1425-1436.
  4. Viator JA., et al. "Gold Nanoparticle Mediated Detection of Prostate Cancer Cells Using Photoacoustic Flowmetry with Optical Reflectance”. Journal of Biomedical Nanotechnology 6 (2010): 187-191.
  5. Kattumuri V., et al. "Gum arabic as a phytochemical construct for the stabilization of gold nanoparticles: In vivo pharmacokinetics and X-ray-contrast-imaging Studies”. Small 3 (2007): 333-341.
  6. Kim YS., et al. "Suppressing effect of resveratrol on the migration and invasion of human metastatic lung and cervical cancer cells”. Molecular Biology Reports 39 (2012): 8709-8716.
  7. Chakraborty A., et al. "In vitro evaluation of the cytotoxic, anti-proliferative and anti-oxidant properties of pterostilbene isolated from Pterocarpus marsupium”. Toxicology In Vitro 24 (2010): 1215-1228.
  8. Sanna V., et al. "Single-step green synthesis and characterization of gold-conjugated polyphenol nanoparticles with antioxidant and biological activities”. International Journal of Nanomedicine 9 (2014): 4935-4951.
  9. Thipe VC., et al. "Development of resveratrol-conjugated gold nanoparticles: interrelationship of increased resveratrol corona on anti-tumor efficacy against breast, pancreatic and prostate cancers”. International Journal of Nanomedicine 14 (2019): 4413-4428.
  10. Cañaveras F., et al. "Role of the Functionalization of the gold nanoparticle surface on the formation of bioconjugates with human serum albumin”. The Journal of Physical Chemistry C 116 (2012): 10430-10437.
  11. Karen Bolaños., et al. "Capping gold nanoparticles with albumin to improve their biomedical properties”. International Journal of Nanomedicine14 (2019): 6387-6406.
  12. Queiroz RG., et al. "Radiation-synthesized protein-based drug carriers: Size-controlled BSA nanoparticles”. International Journal of Biological Macromolecules 85 (2016): 82-91.
  13. Achilli E., et al. "Preparation of protein nanoparticle by dynamic aggregation and ionizing-induced crosslinking”. Colloids and Surfaces A: Physicochemical and Engineering Aspects 486 (2015): 161-171.
  14. ISO/ASTM516-13 Standard Practice for Use of the Alanine-EPR Dosimetry System.
  15. Varca GHC., et al. "Irradiation as an alternative route for protein crosslinking: Cosolvent free BSA nanoparticles”. Radiation Physics and Chemistry 24 (2016): 111-115.
  16. Mohanty R K., et al. "Resveratrol stabilized gold nanoparticles enable surface loading ofdoxorubicin and anticancer activity”. Colloids and Surfaces B: Biointerfaces 114 (2014): 138-143.
  17. Zhao P., et al. "State of the art in gold nanoparticle synthesis”. Coordination Chemistry Reviews 3-4 (2013): 638-665.
  18. Perron NR., et al. "A review of the antioxidant mechanisms of polyphenol compounds related to iron binding”. Cell Biochemistry and Biophysics 53 (2009): 75-100.
  19. Cardoso-Avila P E., et al. "Gold nanoparticles surface modification using BSA and Cysteine”. Proceedings of SPIE 8011 (2011): 801192-801197.
  20. Mariam J., et al. "Albumin corona on nanoparticles - a strategic approach in drug delivery”. Drug Delivery (2015): 1-9.
  21. Asnag G M., et al. "Effect of gamma-irradiation on the structural, optical and electrical properties of PEO/starch blend containing different concentrations of gold nanoparticles”. Radiation Effects and Defects in Solids7-8 (2019): 579-595.
  22. DiMarco T and iulivi C. "Current analytical method for the detection of dityrosine, a biomarker of oxidative stress in biological systems”. Mass Spectrometry Reviews 26 (2006): 108-120.

Citation

Citation: Janaína A G Barros., et al. “Protein Crosslinking Onto Gold Nanoparticles by the Use of Gamma Radiation". Acta Scientific Pharmaceutical Sciences 6.3 (2022): 13-19.

Copyright

Copyright: © 2022 Janaína A G Barros., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.




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