Protein Crosslinking Onto Gold Nanoparticles by the Use of Gamma Radiation
Janaína A G Barros1*, Jorge G S Batista1, Vitor de Moraes Zamarion2 and Ademar B Lugão1
1Instituto de Pesquisas Energéticas e Nucleares (IPEN-CNEN/SP) - Avenida Lineu Prestes, São Paulo, SP, Brazil
2Fundamental Chemistry Department, University of São Paulo (IQ-USP) Avenida Lineu Prestes, São Paulo, SP, Brazil
*Corresponding Author: Janaína A G Barros, Instituto de Pesquisas Energéticas e Nucleares (IPEN-CNEN/SP) - Avenida Lineu Prestes, São Paulo, SP, Brazil.
November 30, 2021; Published: February 21, 2022
Gold nanoparticles (AuNP) are being investigated for diagnostic and therapeutic nanomedicines considering their low toxicity and stability against oxidation, among other features. AuNP may be administered site-specifically or intravenously for diagnostic imaging by computed tomography or for therapy. Albumin functionalization of AuNP whether linked by chemical or physical processes may provide unique features to the system regarding biopharmaceutical aspects. Recently albumin crosslinking has been studied by radiation induced methods. This work aimed the development of radiation crosslinked bovine serum albumin (BSA) capped AuNP for improved administration of the nanoparticles. For such purpose AuNP were synthesized using 2.5 mM sodium tetrachloroaurate (III) dehydrate and 1.67 mM resveratrol in water. The mixture was allowed to stand for 12 hours. AuNP capping was performed by BSA addition followed by slow addition of ice-cold ethanol to reach 5 mg mL-1 BSA in a 30% (v/v) ethanol solution. The mixture was allowed to rest overnight at ± 4 oC. BSA crosslinking onto AuNP was performed by gamma irradiation at doses of 2.5, 5, 7.5, 10 and 15 kGy. AuNP were synthesized with approximately 38 nm, whereas BSA Capped AuNP presented size ranging from 70 nm up to 200 nm, as revealed by dynamic light scattering. AuNP and BSA capped AuNP morphology was evaluated by transmission electron microscopy using negative staining and revealed protein aggregation onto AuNPs and its surroundings. Irradiation led to no shifts in maximum absorbance for both nanoparticles. Protein crosslinking was confirmed by bityrosine and increased with dose.
Keywords: Gold Nanoparticles; Bovine Serum Albumin; Resveratrol; Green Synthesis; BSA Capping; Functionalized Nanoparticles; Protein Crosslinking; Gamma Radiation
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