Acta Scientific Pharmaceutical Sciences (ASPS)(ISSN: 2581-5423)

Research Article Volume 6 Issue 3

The Estimation of Acyclovir in Bulk and Tablet Dosage form by Using Specificity and Analytical Method Development

Sudam G Mule*, Narendra G Patre, Ajay D Kshirsagar and Seema B Kharwade

D.K. Patil Institute of Pharmacy, MH, India

*Corresponding Author: Sudam G Mule, D.K. Patil Institute of Pharmacy, MH, India.

Received: January 11, 2022; Published: February 18, 2022

Abstract

A selective, accurate, HPLC method was developed by this study for the determination of Acyclovir in bulk and tablet dosage form. This method was developed by Thermo Fisher Scientific Software Chromquest Version 4.1 using C18 column in solvents 700 ml of Acetonitrile and 300 ml of buffer pH 3.0 ± 0.10 70:30) HPLC grade were set, C8 (4.6 mm x 1.5 cm, 5 μm) column, flow rate 0.50 ml/ min as mobile phase. the mobile phase was pumped, and the sample was detected at 254 nm. For standard Acyclovir the retention time was 5 min. The method was validated for analytical standards such as linearity, accuracy, precision, and robustness. In a wide range of 5-25 (μg/ml) the linearity was observed.

Keywords: Acyclovir; Specificity; Reagent

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Citation

Citation: Sudam G Mule., et al. “The Estimation of Acyclovir in Bulk and Tablet Dosage form by Using Specificity and Analytical Method Development". Acta Scientific Pharmaceutical Sciences 6.3 (2022): 09-12.

Copyright

Copyright: © 2022 Sudam G Mule., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.




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