The Estimation of Acyclovir in Bulk and Tablet Dosage form by Using
Specificity and Analytical Method Development
Sudam G Mule*, Narendra G Patre, Ajay D Kshirsagar and Seema B
D.K. Patil Institute of Pharmacy, MH, India
*Corresponding Author: Sudam G Mule, D.K. Patil Institute of Pharmacy, MH, India.
January 11, 2022; Published: February 18, 2022
A selective, accurate, HPLC method was developed by this study for the determination of Acyclovir in bulk and tablet dosage form.
This method was developed by Thermo Fisher Scientific Software Chromquest Version 4.1 using C18 column in solvents 700 ml of
Acetonitrile and 300 ml of buffer pH 3.0 ± 0.10 70:30) HPLC grade were set, C8 (4.6 mm x 1.5 cm, 5 μm) column, flow rate 0.50 ml/
min as mobile phase. the mobile phase was pumped, and the sample was detected at 254 nm. For standard Acyclovir the retention
time was 5 min. The method was validated for analytical standards such as linearity, accuracy, precision, and robustness. In a wide
range of 5-25 (μg/ml) the linearity was observed.
Keywords: Acyclovir; Specificity; Reagent
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