Sudam G Mule*, Narendra G Patre, Ajay D Kshirsagar and Seema B Kharwade

D.K. Patil Institute of Pharmacy, MH, India

*Corresponding Author: Sudam G Mule, D.K. Patil Institute of Pharmacy, MH, India.

Received: January 11, 2022; Published: February 18, 2022

Abstract

A selective, accurate, HPLC method was developed by this study for the determination of Acyclovir in bulk and tablet dosage form. This method was developed by Thermo Fisher Scientific Software Chromquest Version 4.1 using C18 column in solvents 700 ml of Acetonitrile and 300 ml of buffer pH 3.0 ± 0.10 70:30) HPLC grade were set, C8 (4.6 mm x 1.5 cm, 5 μm) column, flow rate 0.50 ml/ min as mobile phase. the mobile phase was pumped, and the sample was detected at 254 nm. For standard Acyclovir the retention time was 5 min. The method was validated for analytical standards such as linearity, accuracy, precision, and robustness. In a wide range of 5-25 (μg/ml) the linearity was observed.

Keywords: Acyclovir; Specificity; Reagent

References

1. H J Schaeffer., et al. “9- (2- Hydroxyethoxymethyl) guanine activity against viruses of the herpes group”. Nature5654 (1978): 583-585.
2. Swart KJ., et al. “Automated high-performance liquid chromatographic method for the determination of acyclovir in plasma”. Journal of Chromatography A1 (1994): 65-69.
3. S Ashok Reddy., et al. “Spectrophotometric determination and validation of Acyclovir”. Archives of Applied Science Research 1 (2011): 328-332.
4. G Holkar., et al. “Method Validation and Quantitative determination of antiviral drug Acyclovir in human plasma by a LCMS/MS”. Biological Forum- An International Journal1 (2012): 11-17.
5. H Mascher., et al. “New, high-sensitivity high-performance liquid chromatographic method for the determination of Acyclovir in human plasma, using fluorometric detection”. Journal of Chromatography 583 (1992): 122-127.
6. P Nebinger and M Koel. “Determination of acyclovir by ultra filtration and high performance liquid chromatography”. Journal of Chromatography B: Biomedical Sciences and Applications2 (1993): 342-344.
7. Gurdeep R Chatwal and Sham K Anand. “Instrumental Methods of Chemical Analysis (Analytical Chemistry)”. pg: 2.566-2.567.
8. Douglas A Skoog., et al. “Principles of instrumental analysis, Saunders Golden Sun burst Series”. Philadelphia, 2^nd edition (1980): 725-760.
9. Stulzer HK., et al. “Development and validation of an RP-HPLC method to quantitate acyclovir in cross-linked chitosan microspheres produced by spray drying”. Journal of Chromatography Science6 (2008): 496-500.
10. Basavaiah K., et al. “Simple high-performance liquid chromatographic method for the determination of acyclovir in pharmaceuticals”. Il Farmaco12 (2003): 1301-1306.
11. Arnal J., et al. “Biowaiver Monographs for Immediate Release Solid Oral Dosage Forms: Aciclovir”. Journal of Pharmaceutical Sciences 97 (2008): 5061-5073.
12. https://en.wikipedia.org/wiki/Aciclovir#/media/File:Aciclovir_2D_structure.svg
13. Tzanavaras PD and Themelis DG. “High-throughput HPLC assay of acyclovir and its major impurity guanine using a monolithic column and a flow gradient approach”. Journal of Pharmaceutical and Biomedical Analysis4 (2007): 1526-1530.
14. Manoharan G. “High-pressure liquid chromatographic method development and validation for estimation of Acyclovir in raw and tablet formulation”. Scholars International Journal of Chemistry and Material Sciences1 (2018): 1-5.
15. International Conference on Harmonization of technical Requirements for Registration of Pharmaceuticals for Human use, ICH Harmonized Tripartite guideline-Validation of Analytical procedures: Text and methodology Q2 (R1), Current step 4 version., London (2005).
16. Seema B Kharwade and Narendra G Patre. “Method Development, Validation and Stability Indicating Studies of Rifabutin Using HPLC-DAD”. Acta Scientific Pharmaceutical Sciences11 (2021).
17. Gunasekar Manohara., et al. “Development and Validation of a Stability-Indicating RP-HPLC Method for the Estimation of Acyclovir, in Bulk and Ointment Dosage Form”. International Journal of Pharmaceutical and Phytopharmacological Research (eIJPPR)1 (2019): 11-18.

Citation

Citation: Sudam G Mule., et al. “The Estimation of Acyclovir in Bulk and Tablet Dosage form by Using Specificity and Analytical Method Development". Acta Scientific Pharmaceutical Sciences 6.3 (2022): 09-12.

Copyright

Copyright: © 2022 Sudam G Mule., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.