Mingyue Sun, Weiqiang Xiao and Qingxia Xu*

Department of Clinical Laboratory, Affiliated Cancer Hospital of Zhengzhou University and Henan Cancer Hospital, Zhengzhou, Henan, People's Republic of China

*Corresponding Author: Qingxia Xu, Department of Clinical Laboratory, Affiliated Cancer Hospital of Zhengzhou University and Henan Cancer Hospital, Zhengzhou, Henan, People's Republic of China.

Received: December 14, 2021; Published: January 31, 2022

Abstract

Background: Nosocomial bacterial infections from carbapenem-resistant Klebsiella pneumoniae (CRKP) are associated with high mortality in neurosurgical patients. This study examined the post-neurosurgical meningitis outbreak caused by CRKP of patients with nervous system tumours, and analysed the molecular characteristics of the causative strain.

Methods: Neurosurgical cancer patients with meningitis caused by CRKP between 2017-2019 were retrospectively analysed. Identification of strains and antimicrobial susceptibilities was conducted using BD Phoenix-100, 16S rRNA gene sequencing and broth microdilution. Multilocus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE) were used to identify the subtypes of K. pneumoniae. The genotype correlation, resistance genes and plasmid of isolates were assessed by whole-genome sequencing (WGS).

Results: Isolates were resistant to almost all of the tested antimicrobial agents except polymyxin and tigecycline. The PFGE and MLST revealed all isolates were the same strain - ST11-while WGS phylogenetic analysis indicated they were closely related. The isolates harboured blaKPC-2 and an IncFII-type plasmid; the blaKPC-2 gene had a similar genetic environment across isolates.

Conclusions: The results of molecular analysis showed that ST11 and IncFII-type plasmid in CRKP have close correlations and indicate a long-term retrospective genomic study throughout the hospital for KPC-producing K. pneumoniae is necessary.

Key words: K. pneumoniae; Meningitis; Whole-Genome Sequencing; Incfii-Type Plasmid

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Citation

Citation: Qingxia Xu., et al. “Whole-Genome Analysis of KPC-Producing Klebsiella Pneumoniae Isolates from Hospital Acquired Post-Neurosurgical Meningitis". Acta Scientific Pharmaceutical Sciences 6.2 (2022): 20-29.

Copyright

Copyright: © 2022 Qingxia Xu., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.