Acta Scientific Pharmaceutical Sciences (ASPS)(ISSN: 2581-5423)

Research Article Volume 5 Issue 9

Vitamin E Improves Sperm Quality of Chlamydia trachomatis Infected Mice

Nasrin Kheradmand1, Seyed Danial Mohammadi2, Negin Nejati3, Arash Babaei1, Fatemeh Ouroujzadeh4 and Maryam Rahimi1*

1Department of Biology, Faculty of Sciences, Malayer University, Malayer, Iran
2Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
3Department of Nursing and Midwifery, Shahid Beheshti University of Medical Sciences, Tehran, Iran
4Department of Animal Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran

*Corresponding Author: Maryam Rahimi, Department of Biology, Faculty of Sciences, Malayer University, Malayer, Iran.

Received: June 28, 2021; Published: August 04, 2021

Abstract

Chlamydia trachomatis (CT) is the most dominant sexually transmitted infection of the male genital tract which impairs semen quality and male fertility. Regarding to high DNA fragmentation of sperm in CT infection, it has been proposed that using the antioxidant agents such as vitamin E may ameliorate semen quality of CT infected patients.

The aim of this study is to investigate the effects of vitamin E administration on sperm quality improvement in CT infected mice.

Twenty four adult mice were distributed in four groups: control, CT infected, CT infected + vitamin E (100 mg/kgbody weight/day), and vitamin E (100 mg/kgbody weight/day) groups. Five days after infection induction, animals were treated with vitamin E for 3 weeks. Then animals were killed and the testicles were removed for sperm analysis, gene expression and western blot experiments. Furthermore, serum levels of sexual hormones were evaluated.

CT infection caused a significant reduction in testes weight, testosterone level, and sperm parameters including number, motility, and vitality. Moreover, follicle-stimulating hormone (FSH) level and expression of p53 were significantly higher in CT infected mice in compared with the control group (p < 0.05). Our results showed that sperm parameters, p53 expression, and sexual hormones levels in CT infected + vitamin E group had no significant difference compared with the control group.

Vitamin E can be an effective therapeutic agent to improve sperm parameters in CT infected patients possibly via its antioxidant properties.

Keywords: Chlamydia trachomatis; Sperm Parameters; Vitamin E

References

  1. Jodar M., et al. “Semen proteomics and male infertility”. Journal of Proteomics 162 (2017): 125-134.
  2. Roozbahani GA., et al. “Association study of SPATA-16 polymorphism with male infertility in Iranian population”. Meta Gene 13 (2017): 154-158.
  3. Bosilkovski M., et al. “Testicular infection in brucellosis: Report of 34 cases”. Journal of Microbiology, Immunology and Infection 1 (2018): 82-87.
  4. Suarez JP., et al. “Chlamydia trachomatis neither exerts deleterious effects on spermatozoa nor impairs male fertility”. Scientific Reports1 (2017): 1126.
  5. Khoshakhlagh A., et al. “Comparison the diagnostic value of serological and molecular methods for screening and detecting Chlamydia trachomatis in semen of infertile men: A cross-sectional study”. International Journal of Reproductive Biomedicine (Yazd) 15 (12): 763-770.
  6. Wagenlehner FM., et al. “Urinary tract infections and bacterial prostatitis in men”. Current Opinion in Infectious Diseases 1 (2014): 97-101.
  7. Eley A., et al. “Can Chlamydia trachomatis directly damage your sperm?” The Lancet Infectious Diseases1 (2005): 53-57.
  8. Satta A., et al. “Experimental Chlamydia trachomatis infection causes apoptosis in human sperm”. Human Reproduction Update 1 (2006): 134-137.
  9. Hosseinzadeh S., et al. “Co-incubation of human spermatozoa with Chlamydia trachomatis serovar E causes premature sperm death”. Human Reproduction Update 2 (2001): 293-299.
  10. Segnini A., et al. “Chlamydia trachomatis and sperm lipid peroxidation in infertile men”. Asian Journal of Andrology1 (2003): 47-49.
  11. Taylor BD and Haggerty CL. “Management of Chlamydia trachomatis genital tract infection: screening and treatment challenges”. Infection and Drug Resistance 4 (2011): 19-29.
  12. Elder DP., et al. “Antibiotic Resistance: The Need For a Global Strategy”. Journal of Pharmaceutical Sciences 8 (2016): 2278-2287.
  13. Walczak–Jedrzejowska R., et al. “The role of oxidative stress and antioxidants in male fertility”. Central European Journal of Urology1 (2013): 60.
  14. Bernstein-Hanley I., et al. “Genetic analysis of susceptibility to Chlamydia trachomatis in mouse”. Genes Immunology2 (2006): 122-129.
  15. Klawitter J., et al. “Urine metabolites reflect time-dependent effects of cyclosporine and sirolimus on rat kidney function”. Chemical Research in Toxicology1 (2009): 118-128.
  16. Sadeghzadeh F., et al. “Vitamin C ameliorates the adverse effects of dexamethasone on sperm motility, testosterone level, and spermatogenesis indexes in mice”. Human and Experimental Toxicology 4 (2019): 409-418.
  17. Kyjovska ZO., et al. “Daily sperm production: application in studies of prenatal exposure to nanoparticles in mice”. Reproduction and Toxicology 36 (2013): 88-97.
  18. Wolff H., et al. “Chlamydia trachomatis induces an inflammatory response in the male genital tract and is associated with altered semen quality”. Fertility and Sterility5 (1991): 1017-1019.
  19. Keck C., et al. “Seminal tract infections: impact on male fertility and treatment options”. Human Reproduction Update 6 (1998): 891-903.
  20. Beumer TL., et al. “The role of the tumor suppressor p53 in spermatogenesis”. Cell Death and Differentiation 8 (1998): 669-677.
  21. Shaha C. “Modulators of spermatogenic cell survival”. Society for Reproduction and Fertility 63 (2007): 173-186.
  22. Cohen-Bacrie P., et al. “Correlation between DNA damage and sperm parameters: a prospective study of 1,633 patients”. Fertility and Sterility5 (2009): 1801-1805.
  23. Moradi MN., et al. “Evaluation of the p53 and Thioredoxin reductase in sperm from asthenozoospermic males in comparison to normozoospermic males”. Free Radical Biology and Medicine 116 (2018): 123-128.
  24. Gallegos G., et al. “Sperm DNA fragmentation in infertile men with genitourinary infection by Chlamydia trachomatis and Mycoplasma”. Fertility and Sterility2 (2008): 328-334.
  25. Al-Mously N., et al. “Real-time polymerase chain reaction shows that density centrifugation does not always remove Chlamydia trachomatis from human semen”. Fertility and Sterility5 (2009): 1606-1615.
  26. Hosseinzadeh S., et al. “Semen quality of men with asymptomatic chlamydial infection”. Journal of Andrology1 (2004): 104-109.
  27. Keshtgar S., et al. “In vitro effects of alpha-tocopherol on teratozoospermic semen samples”. Andrologia 44 (2012): 721-727.
  28. Yousef MI. “Vitamin E modulates reproductive toxicity of pyrethroid lambda-cyhalothrin in male rabbits”. Food and Chemical Toxicology 5 (2010): 1152-1159.
  29. Khorramabadi KM., et al. “Protective effect of vitamin E on oxidative stress and sperm apoptosis in diabetic Mice”. International Journal of Reproductive BioMedicine2 (2019).
  30. Suleiman SA., et al. “Lipid peroxidation and human sperm motility: protective role of vitamin E”. Journal of Andrology5 (1996): 530-537.
  31. Momeni HR and Eskandari N. “Effect of vitamin E on sperm parameters and DNA integrity in sodium arsenite-treated rats”. Iranian Journal of Reproductive Medicine 3 (2012): 249-256.
  32. Ener K., et al. “The impact of vitamin E supplementation on semen parameters and pregnancy rates after varicocelectomy: a randomised controlled study”. Andrologia 7 (2016): 829-834.
  33. Silver EW., et al. “Effect of antioxidant intake on sperm chromatin stability in healthy nonsmoking men”. Journal of Andrology4 (2005): 550-556.

Citation

Citation: Maryam Rahimi., et al. “Vitamin E Improves Sperm Quality of Chlamydia trachomatis Infected Mice". Acta Scientific Pharmaceutical Sciences 5.9 (2020): 17-24.

Copyright

Copyright: © 2020 Maryam Rahimi., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.




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