Biocompatible Antidermatophytic Scaffolds (TfG-Nf) for Controlled and Impressive Management of Topical Tinea Diseases
Shashi Kiran Misra1*, Himanshu Pandey2, Kamla Pathak3 and Sandip Patil4
1University Institute of Pharmacy, CSJMU, Kanpur, India
2Central University of Higher Tibetan Studies, Varanasi, India
3Faculty of Pharmacy, Uttar Pradesh University of Medical Sciences, Saifai, Uttar Pradesh, India
4E-Spin NanoTech Private Ltd., SIDBI Innovation and Incubation Center, Indian Institute of Technology, Kanpur, India
*Corresponding Author: Shashi Kiran Misra, University Institute of Pharmacy, CSJMU, Kanpur, India.
May 24, 2021; Published: June 03, 2021
Purpose: The rationale behind this study was to develop graphene based tolnaftate conjugate (Tf G) and embed it within scaffolds composed of biocompatible Eudragit polymers for controlled and impressive antitinea activity.
Methods: Developed Tf G conjugate was entrenched in 20% w/v polymeric solution (ERL100/ERS100) and nonwoven scaffolds through E-spin (electrospinning) technology were fabricated. Assorted analytical techniques i.e. FESEM, FTIR, XRD and DSC were employed to characterize and assessment of scaffolds. Sessile drop and Dialysis bag methods were utilized for the determination of their hydrophilicity and drug release behavior. In vitro fungal study was performed on enormously virulent strains of tinea infections i.e. zoophilic Microsporum fulvum and anthropophilic Trichophyton rubrum. Animal study was performed on Trichophyton rubrum diseased Swiss albino mice for seven days.
Results: High payload of Tf on G was perceived by the virtue of extensive surface area of G and process of physisorption. Surface morphology analyzed that TfG-Nf were nano-dimensional (90-120 nm), uniform, defect-free and possessing numerous pores. Tremendous swelling capacity and pronounced hydrophilicity were obtained that gratified crucial benchmarks for scaffolds to alleviate tinea infections. In vitro drug release data interpreted controlled diffusion of drug for 8 hrs. Microdilution assay exhibited remarked eradication (99.356%) of viable cells of T. rubrum after 96 hrs. Animal study on infected Swiss albino mice exposed better antitinea activity of TfG-Nf amid new growth of hair at infected site.
Conclusion: Fabricated TfG-Nf exhibited superior in vitro and in vivo action than Tf- Nf scaffolds and offered promising topical drug delivery approach to mitigate superficial bugs and heal up at infected regions. Findings also endorse newer biomedical application of graphene in designing wound dressings (bandages/scaffolds) materials.
Keywords: Tolnaftate; Graphene; Nano-dimensional; Scaffolds; Tinea Disease; Eudragit; Trichophyton rubrum
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