Acta Scientific Pharmaceutical Sciences (ASPS)(ISSN: 2581-5423)

Research Article Volume 5 Issue 7

Biocompatible Antidermatophytic Scaffolds (TfG-Nf) for Controlled and Impressive Management of Topical Tinea Diseases

Shashi Kiran Misra1*, Himanshu Pandey2, Kamla Pathak3 and Sandip Patil4

1University Institute of Pharmacy, CSJMU, Kanpur, India
2Central University of Higher Tibetan Studies, Varanasi, India
3Faculty of Pharmacy, Uttar Pradesh University of Medical Sciences, Saifai, Uttar Pradesh, India
4E-Spin NanoTech Private Ltd., SIDBI Innovation and Incubation Center, Indian Institute of Technology, Kanpur, India

*Corresponding Author: Shashi Kiran Misra, University Institute of Pharmacy, CSJMU, Kanpur, India.

Received: May 24, 2021; Published: June 03, 2021


Purpose: The rationale behind this study was to develop graphene based tolnaftate conjugate (Tf G) and embed it within scaffolds composed of biocompatible Eudragit polymers for controlled and impressive antitinea activity.

Methods: Developed Tf G conjugate was entrenched in 20% w/v polymeric solution (ERL100/ERS100) and nonwoven scaffolds through E-spin (electrospinning) technology were fabricated. Assorted analytical techniques i.e. FESEM, FTIR, XRD and DSC were employed to characterize and assessment of scaffolds. Sessile drop and Dialysis bag methods were utilized for the determination of their hydrophilicity and drug release behavior. In vitro fungal study was performed on enormously virulent strains of tinea infections i.e. zoophilic Microsporum fulvum and anthropophilic Trichophyton rubrum. Animal study was performed on Trichophyton rubrum diseased Swiss albino mice for seven days.

Results: High payload of Tf on G was perceived by the virtue of extensive surface area of G and process of physisorption. Surface morphology analyzed that TfG-Nf were nano-dimensional (90-120 nm), uniform, defect-free and possessing numerous pores. Tremendous swelling capacity and pronounced hydrophilicity were obtained that gratified crucial benchmarks for scaffolds to alleviate tinea infections. In vitro drug release data interpreted controlled diffusion of drug for 8 hrs. Microdilution assay exhibited remarked eradication (99.356%) of viable cells of T. rubrum after 96 hrs. Animal study on infected Swiss albino mice exposed better antitinea activity of TfG-Nf amid new growth of hair at infected site.

Conclusion: Fabricated TfG-Nf exhibited superior in vitro and in vivo action than Tf- Nf scaffolds and offered promising topical drug delivery approach to mitigate superficial bugs and heal up at infected regions. Findings also endorse newer biomedical application of graphene in designing wound dressings (bandages/scaffolds) materials.

Keywords: Tolnaftate; Graphene; Nano-dimensional; Scaffolds; Tinea Disease; Eudragit; Trichophyton rubrum


  1. Peres NT., et al. “Dermatophytes: host pathogen interaction and antifungal resistance”. Anais Brasileiros de Dermatologia 5 (2010): 657-667.
  2. Araujo CR., et al. “In Vitro susceptibility testing of dermatophytes isolated in Goiania, Brazil, against five antifungal agents by broth microdilution method”. Revista do Instituto de Medicina Tropical de São Paulo 1 (2009): 9-12.
  3. Bergus GR., et al. “Superficial tinea infections”. American Family Physician 48 (1993): 259-268.
  4. Lakshmipathy DK., et al. “Review on dermatomycosis: pathogenesis and treatment”. Natural Science 7 (2010): 726-731.
  5. Kaplan W., et al. “Ringworm in the dog caused by Trichophyton rubrum”. Veterinary Medicine 53 (1958): 139-142.
  6. Grappel SF., et al. “Immunology of dermatophytes and dermatophytosis”. Bacteriology Reviews 3 (1974): 222-250.
  7. Perea S., et al. “Antifungal resistance in pathogenic fungi”. Clinical Infectious Diseases 9 (2002): 1073-1080.
  8. Berdy J., et al. “Bioactive Metabolites from Microorganisms”. Elsevier, Amsterdam (2005): 3-25.
  9. Dhivya S., et al. “Wound dressings- a review”. Biomedicine4 (2015): 22-29.
  10. Camposeo A., et al. “Local mechanical properties of electrospun fibres correlate to their internal nanostructure”. Nano Letter11 (2013): 5056-5062.
  11. Lin J., et al. “Co-electrospun nanofibrous membranes of collagen and zein for wound healing”. ACS Applied Materials and Interfaces 2 (2012): 1050-1057.
  12. Iwata K., et al. “In vitro thiocarbamate resistance of trichophyton mentagrophytes”. Chemotherapy 5 (1990): 435-443.
  13. Yamaguchi H., et al. “Therapeutic efficacy of a topical tolnaftate preparation in guinea pig model of tinea pedis”. Japanese Journal of Antibiotics 6 (2001): 323-330.
  14. Akhavan O., et al. “Toxicity of graphene and graphene oxide nanowalls against bacteria”. ACS Nano 10 (2010): 5731-5736.
  15. Kalbacova M., et al. “Graphene substrates promote adherence of human osteoblasts and mesenchymal stromal cells”. Carbon 15 (2010): 4323-4329.
  16. Nayak TRS., et al. “Graphene for controlled and accelerated osteogenic differentiation of human mesenchymal stem cells”. ACS Nano 6 (2011): 4670-4678.
  17. , et al. “Behaviours of NIH-3T3 fibro-blasts on graphene /caron nanotubes: proliferation, focal adhesion, and gene transfection studies”. ACS Nano 4.11 (2010): 6587-6598.
  18. Novoselov KS., et al. “Electric field effect in atomically thin carbon films”. Science 5696 (2004): 666-669.
  19. Bolotin KI., et al. “Ultrahigh electro mobility in suspended graphene”. Solid State Communication 14 (2008)6: 351-355.
  20. Sundaray B., et al. “Preparation and characterization of electrospun fibres of poly (methyl methacrylate) single walled carbon nanotube nanocomposites”. Journal of Engineered Fibers and Fabrics 4 (2008): 39-45.
  21. Kam NWS., et al. “Nanotube molecular transporters internalization of carbon nanotube- protein conjugates into mammalian cells”. Journal of the American Chemical Society 22 (2004): 6850-6851.
  22. Li N., et al. “Flexible graphene- based lithium ion batteries with ultrafast charge and discharge rates”. Proceedings of the National Academy of Sciences of the United States of America 109 (2012): 17360-17365.
  23. Pandey H. “Controlled drug release characteristics and enhanced antibacterial effect of graphene nanosheets containing gentamicin sulfate”. Nanoscale 3 (2011): 4104-1408.
  24. Kumari P., et al. “Formulation and evaluation of tolnaftate microsponges loaded gels for treatment of dermatophytosis”. European Journal of Pharmaceutical and Medical Research 6 (2017): 326-335
  25. Dillen K., et al. “Evaluation of Ciprofloxacin Loaded Eudragit RS 100 or RL 100/plga Nanoparticles”. International Journal of Pharmaceutics 314 (2006): 72-82.
  26. Jiang X., et al. “Plasmid templated shape control of condensed DNA- block copolymer nanoparticles”. Advances in Materials 25 (2013): 227-232.
  27. Vardharajula S., et al. “Functionalized carbon nanotubes: biomedical applications”. International Journal of Nanomedicine 7 (2012): 5361-5374.
  28. Liu Z., et al. “Pegylated nano- graphene oxide for delivery of water insoluble cancer drugs”. Journal of the American Chemical Society 33 (2008): 10876-10877.
  29. Ravelli D., et al. “PEGylated carbon nanotubes: preparation, properties and applications”. RSC Advances 3 (2013): 13569-13582.
  30. Misra SK., et al. “Carbon Allotrope Graphene: Superstar In Nano- World”. International Journal of Advances in Pharmaceutical Sciences 1 (2013): 1-4.
  31. Karthikeyana K., et al. “Electrospun zein/eudragit nanofibres based dual drug delivery system for the simultaneous delivery of aceclofenac and pantoprazole”. International Journal of Pharmaceutics 438 (2012): 117-122.
  32. Lin J., et al. “Co-electrospun nanofibrous membranes of collagen and zein for wound healing”. ACS Applied Materials and Interfaces 4 (2012): 1050-1057.
  33. Huang FL., et al. “Dynamic wettability and contact angles of poly (vinylidene fluoride) nanofiber membranes grafted with acrylic acid”. eXPRESS Polymer Letters9 (2010): 551-558.
  34. Castelli F., et al. “Flurbiprofen release from Eudragit RS and RL aqueous nanosuspensions: a kinetic study by DSC and dialysis experiments”. AAPS Pharm Sci Tech2 (2002): 1-8.
  35. Martinez –Rossi NM., et al. “Role of pH in the pathogenesis of dermatophytoses”. Mycoses 55 (2012): 381-387.
  36. Ahmed A., et al. “Nanofiber composites containing N-heterocyclic carbene complexes with antimicrobial activity”. International Journal of Nanomedicine 7 (2012): 2829-2832.
  37. Pandey S., et al. “Synthesis and characterization of graphene-usnic acid conjugate microspheres and its antibacterial activity against Staphylococcus aureus”. IJPSR 2 (2019): 1000-1008.
  38. Chen J., et al. “Graphene oxide exhibits broad-spectrum antimicrobial activity against bacterial phytopathogens and fungal conidia by interwining and membrane perturbation”. Nanoscale 3 (2014): 1879-1889.
  39. Misra SK., et al. “Tolnaftate loaded polyacrylate electrospun nanofibers for impressive regimen on dermatophytosis”. Fibers 5 (2017): 41-52.
  40. Sharma N., et al. "Smart Graphene-Keto Nano hybrids Loaded Therapeutics for Effective Management Dermatophytosis". Drug Delivery Letter1 (2019): 21-28.
  41. Padhan DK., et al. “In Vivo antifungal activity of Accmella essential oil on a dermatomycotic strain Trichophyton mentagrophytes (MTCC-7687)”. Pelagia Research Library, Der Pharmacia Sinica 1 (2014): 40-44.
  42. Huang C., et al. “Fiber, shape structure and surface texture”. Journal of Nanomedicine (2012): 1-7.
  43. Misra SK., et al. “Tolnaftate- graphene composite loaded nanoengineered electrospun scaffods as efficient therapeutic dressing material for regimen of dermatomycosis”. Applied Nanoscience 7 (2018): 1629-1640.


Citation: Shashi Kiran Misra., et al. “Biocompatible Antidermatophytic Scaffolds (TfG-Nf) for Controlled and Impressive Management of Topical Tinea Diseases". Acta Scientific Pharmaceutical Sciences 5.7 (2020): 08-19..


Copyright: © 2020 Shashi Kiran Misra., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


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